功能菌剂在制革废水不同处理单元的代谢差异
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摘要
为了解外源菌剂在处理系统中的微生物活性和群落结构变化对于制革废水处理效果的影响。采用Biolog技术追踪了不同处理单元活性污泥中菌群的代谢差异性变化,获得了菌群代谢指纹图谱和功能群落多样性信息,并结合COD、氨氮及总氮去除率进行综合分析。研究结果表明,各单元功能菌群均对醇类碳源的代谢衰退最显著,而综合代谢活性衰退在二级好氧池最明显;各单元功能群落丰富度高而代谢活性较低是由菌剂稀释造成的,投加量是影响废水处理效果的关键因素,在生物选择池增大菌剂投加量可最大限度地减少菌剂总的使用量,并使COD、氨氮及总氮去除率分别提高23.1%、58.8%及43.5%。
The effect of microbial activity and community structure of exogenous microbial agents in treatment system was investigated. The metabolic activity of activated sludge in different treatment units were analyzed by Biolog method and ob-tained their bacterial metabolic fingerprints and functional community diversity. The COD, ammonia nitrogen and total nitro-gen removal rate were investigated as well. The results showed that the metabolic decline of alcoholic carbon source was the most pronounced in each unit, and the decline of comprehensive metabolic activity was most obvious in secondary aerobic tank. The dilution of microbial agents caused high richness but low metabolic activity of the functional communities. The dosage of microbial agents was the key factor influencing the wastewater treatment effect. Increasing the amount of microbial agents in the biological selection pool can minimize its consumption, and the removal rate of COD, ammonia nitrogen and to-tal nitrogen increased by 23.1%, 58.8% and 43.5% respectively.
The effect of microbial activity and community structure of exogenous microbial agents in treatment system was investigated. The metabolic activity of activated sludge in different treatment units were analyzed by Biolog method and ob-tained their bacterial metabolic fingerprints and functional community diversity. The COD, ammonia nitrogen and total nitro-gen removal rate were investigated as well. The results showed that the metabolic decline of alcoholic carbon source was the most pronounced in each unit, and the decline of comprehensive metabolic activity was most obvious in secondary aerobic tank. The dilution of microbial agents caused high richness but low metabolic activity of the functional communities. The dosage of microbial agents was the key factor influencing the wastewater treatment effect. Increasing the amount of microbial agents in the biological selection pool can minimize its consumption, and the removal rate of COD, ammonia nitrogen and to-tal nitrogen increased by 23.1%, 58.8% and 43.5% respectively.
引文
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[19]孔艳,江洪,张秀英,等.基于Holdridge和CCA分析的中国生态地理分区的比较[J].生态学报,2013,33(12):3827-3836.Kong Yan,Jiang Hong,Zhang Xiuying,et al.The comparision of ecological geographica regionlization in China based on Holdridge and CCA analysis[J].Acta Ecologica Sinica,2013,33(12):3827-3836.
[2]宋景华,李谷,张世羊,等.循环水养殖池塘微生物群落的碳源代谢特性和功能多样性[J].江苏农业科学,2013,41(9):305-309.Song Jinghua,Li Gu,Zhang Shiyang,et al.Carbon source metabolic characteristics and functional diversity of microbial communities in circulating aquaculture ponds[J].Jiangsu Agricultural Science,2013,41(9):305-309.
[3]赵艳,李锋民,王昊云,等.不同结构好氧/厌氧潜流人工湿地微生物群落代谢特性[J].环境科学学报,2012,32(2):299-307.Zhao Yan,Li Fengmin,Wang Haoyun,et al.Characteristics of microbial community metabolism in aerobic/anaerobic subsurface flow constructed wetland[J].Acta Scientiae Circumstantiae,2012,32(2):299-307.
[4]邓欢欢,葛利云,顾国泉,等.垂直流人工湿地基质微生物群落的代谢特性和功能多样性研究[J].水处理技术,2007,33(6):18-21.Deng Huanhuan,Ge Liyun,Gu Guoquan,et al.Metabolic characteristics and functional diversity of microbial community in vertical flow constructed wetland[J].Technology of Water Treatment,2007,33(6):18-21.
[5]邓欢欢,葛利云,蒋愉林,等.水平潜流人工湿地微生物群落的碳源代谢特性和功能多样性研究[J].农业环境科学学报,2007,26(6):2144-2149.Deng Huanhuan,Ge Liyun,Jiang Yulin,et al.Analysis of the carbon metabolic properties and functional diversity of the microbial community in horizontal sub-surface flow constructed wetlands[J].Journal of Agro-environment Science,2007,26(6):2144-2149.
[6]文娅,赵国柱,周传斌,等.一种新型微生物菌剂处理生活污水[J].环境生物学报,2013,7(5):1730-1734.Wen Ya,Zhao Guozhu,Zhou Chuanbin,et al.Treatment of domestic sewage by a new microbial agent[J].Chinese Journal of Environmental Engineering,2013,7(5):1730-1734.
[7]Kong X,Wang C,Ji M.Analysis of microbial metabolic characteristics in mesophilic and thermophilic biofilters using Biolog plate technique[J].Chemical Engineering Journal,2013,230(10):415-421.
[8]Yang C,Zhang W,Liu R H,et al.Phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-wastewater treatment plants[J].Environ Sci Technol,2011,45(10):7408-7415.
[9]王伟,徐艳,侯昭牧,等.Ni2+对活性污泥活性及群落多样性的影响[J].环境工程学报,2014,8(1):138-143.Wang Wei,Xu Yan,Hou Zhaomu,et al.Effects of Ni(Ⅱ)on activity and community diversity of activated sludge[J].Journal of Environmental Engineering,2014,8(1):138-143.
[10]Gryta A,Frac M,Oszust K.The application of the Biolog Eco Plate approach in ecotoxicological evaluation of dairy sewage sludge[J].Applied Biochemistry and Biotechnology,2014,174(4):1434-1443.
[11]刘峰,张兰英,刘鹏,等.采用Biolog法分析制药废水处理工艺中微生物多样性[J].中国给水排水,2007,23(21):28-32.Liu Feng,Zhang Lanying,Liu Peng,et al.Biolog method for analysis of microbial diversity during treatment of pharmaceutical wastewater[J].China Water&Wastewater,2007,23(21):28-32.
[12]Garland J L,M ills A L.Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J].Appl Environ Microbial,1991,57(8):2351-2359.
[13]Gavrilescu M.Environmental biotechnology:achievements,opportunities and challenges[J].Dynamic Biochemistry,Process Biotechnology and Molecular Biology,2010,4(1):1-36.
[14]张燕燕,曲来叶,陈利顶.Biolog Eco Plate TM实验信息提取方法改进[J].微生物学通报,2009,36(7):1083-1091.Zhang Yanyan,Qu Laiye,Chen Liding.An amendment on information extraction of Biolog Eco PlateTM[J].Microbiology China,2009,36(7):1083-1091.
[15]Garland J L.Analysis and interpretation of community-level physiological profiles in microbial ecology[J].FEMS Microbiology Ecology,1997,24(4):289-300.
[16]Zak J C,Willing M R,Moorhead D L,et al.Functional diversity of microbial communities:a quantitative approach[J].Soil Biology and Biochemistry,1994,26(9):1101-1108.
[17]贾夏,董岁明,周春娟.微生物生态研究中Biolog Eco微平板培养时间对分析结果的影响[J].应用基础与工程科学学报,2013,21(1):10-19.Jia Xia,Dong Suiming,Zhou Chunjuan.Effects of Biolog Eco microplate culture time on analysis results in microbial ecology study[J].Journal of Basic Science and Engineering,2013,21(1):10-19.
[18]张海涵,唐明,陈辉,等.不同生态条件下油松(Pinus tabuliformis)菌根根际土壤微生物群落[J].生态学报,2007,27(12):5463-5470.
[19]孔艳,江洪,张秀英,等.基于Holdridge和CCA分析的中国生态地理分区的比较[J].生态学报,2013,33(12):3827-3836.Kong Yan,Jiang Hong,Zhang Xiuying,et al.The comparision of ecological geographica regionlization in China based on Holdridge and CCA analysis[J].Acta Ecologica Sinica,2013,33(12):3827-3836.