苗药阿锐布对四氯化碳致小鼠急性肾损伤的保护作用
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摘要
目的:初步探讨苗药阿锐布对四氯化碳致肾损伤的保护作用。方法:50只小鼠被随机分为空白组、模型组及阿锐布低、中、高剂量组,2周后处死小鼠,肾石蜡切片,HE染色,检测肾组织匀浆中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,计算肾脏指数,光镜下观察肾组织形态结构变化。结果:一定剂量的阿锐布水提物能提高肾组织匀浆中SOD水平(P<0.01或P<0.05),降低肾组织匀浆中MDA水平(P<0.01)。结论:苗药阿锐布对四氯化碳所致小鼠肾损伤有较好的防治作用,作用机制可能与其所含活性物质的抗氧化作用有关。
Objective To investigate the protective effect of the Veronicastrum axillare on carbon tetrachloride-induced renal injury in mice. Method 50 mice were randomly divided into normal group,model group and the low、middle and high dose group,mice were sacrificed at the 2 th week,After the kidney paraffin were sected,and stained with HE,detection SOD activities and MDA level in renal tissue homogenate,calculation kidney index,the morphological changes in renal tissue were observed under microscope. Result A certain dose of the Veronicastrum axillare water extract can increase SOD level in renal tissue homogenate(P<0.01 or P<0.05)and decrease MDA level in renal tissue homogenate(P<0.01). Conclusion Miao medicine the Veronicastrum axillare have better protection effect on carbon tetrachloride-induced mice renal injury.The mechanism may be related to the antioxidant effect of the active substance.
Objective To investigate the protective effect of the Veronicastrum axillare on carbon tetrachloride-induced renal injury in mice. Method 50 mice were randomly divided into normal group,model group and the low、middle and high dose group,mice were sacrificed at the 2 th week,After the kidney paraffin were sected,and stained with HE,detection SOD activities and MDA level in renal tissue homogenate,calculation kidney index,the morphological changes in renal tissue were observed under microscope. Result A certain dose of the Veronicastrum axillare water extract can increase SOD level in renal tissue homogenate(P<0.01 or P<0.05)and decrease MDA level in renal tissue homogenate(P<0.01). Conclusion Miao medicine the Veronicastrum axillare have better protection effect on carbon tetrachloride-induced mice renal injury.The mechanism may be related to the antioxidant effect of the active substance.
引文
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[10] 周伯旭,孟宪纾.轮叶婆婆纳的药理研究[J].中国中药杂志,1992,17(8):493-496.
[11] 党璇,赵兵,高昂,等.腹水草属药学研究[J].安徽农业科学,2011,39(32):19801-19802.
[2] 吴金辉.腹水草治疗急慢性肾炎[J].浙江中医杂志,1997(11):516.
[3] 唐海华.苗药药物学[M].贵阳:贵州民族出版社,2006:202.
[4]Hermenean A,Ardelean A,Stan M,et al.Protective effects of naringenin on carbon tetrachloride-induced acute nephrotoxicity in mouse kidney[J].Chemistry Biology Interaction,2013,205(2):138-147.
[5] SUZUKI K,NAKAGAWA K,YAMAMOTO T,et al.Carbon tetrachloride-induced hepatic and renal damages in rat:inhibitory effects of cacao polyphenol[J].Bioscience Biotechnology and Biochemistry,2015,79(10):1669-1675.
[6]WANG Xiaohong,ZHANG Weiguang,Tian Long.Study on the apoptosis of renal xells of carbon tetrachloride induced rats[J].Acta anatomicasinica,2006,37(6):673-676.
[7] RUPRAH M,MANT T G,FLANAGAN R J.Acute carbon tetrachloride poisoning in 19 patients:implications for diagnosis and treatment[J].L.ancet,1985,1(8436):1027-1029.
[8] Koruk M,Taysi S,Savas MC,et al.Oxidative stress and enzymatic antioxidant status in patients with nonalcoholic steatohepatitis[J].Acta Clin Lab Sci,2004,34(2):57-62.
[9] Alkushi AG.Protective effect of sorrel extract on adult rats treated by carbon tetrachloride[J].Pharmacognosy Res,2017,9(2):200-207.
[10] 周伯旭,孟宪纾.轮叶婆婆纳的药理研究[J].中国中药杂志,1992,17(8):493-496.
[11] 党璇,赵兵,高昂,等.腹水草属药学研究[J].安徽农业科学,2011,39(32):19801-19802.