盐度对虹鳟和硬头鳟幼鱼消化酶和抗氧化酶活性的比较研究
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摘要
为比较研究不同盐度下虹鳟(Oncorhynchus mykiss)和硬头鳟(O.mykiss)幼鱼的消化和抗氧化能力的差异,在7个盐度(0、5、10、15、20、25和30,分别以S0、S5、S10、S15、S20、S25和S30表示)下饲养虹鳟(3.55g)和硬头鳟(3.57g)幼鱼各40d,分析其肠、胃消化酶和肝脏抗氧化酶活性。每个处理组3个重复,每个重复放养8尾鱼。实验用水由自然海水和淡水调配而成,以升盐率1～2d-1升至设置盐度后稳定7d进行实验。研究显示:虹鳟和硬头鳟的肠淀粉酶、肠脂肪酶、肠胰蛋白酶、胃淀粉酶和胃蛋白酶活性分别在S5和S10组达到峰值,且显著高于其它组。在S5组,虹鳟的肠、胃消化酶活性均显著高于硬头鳟,在S10组,硬头鳟的肠、胃消化酶活性均显著高于虹鳟。虹鳟和硬头鳟的肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量在S5或S10组达到峰值;在S25和S30组,硬头鳟的肝脏SOD、CAT、谷胱甘肽过氧化物酶(GSH-PX)活性和MDA含量显著高于虹鳟。研究结果表明,虹鳟和硬头鳟幼鱼分别在盐度5和10时消化能力最好,在高盐度(25～30)下硬头鳟幼鱼的消化和抗氧化能力优于虹鳟。
In order to compare the digestive and antioxidant abilities of juvenile rainbow and steelhead trout at different salinities,juvenile rainbow(average body weight 3.55 g)and steelhead trout(average body weight 3.57 g)were reared at 7 different salinities(0,5,10,15,20,25 and 30,abbreviated as S0,S5,S10,S15,S20,S25 and S30)for 40 d,respectively,three tanks each salinity and 8 individuals each tank.We analyzed the intestinal and gastric digestive and liver antioxidant enzyme activities of the fish individuals.The salinity was gradually adjusted with natural seawater and fresh water at a rate of1～2 d-1 and stabilized for 7 days.The results showed that the activities of intestinal amylase,lipase and trypsin and gastric amylase and pepsin in rainbow and steelhead trout peaked at S5 and S10,which were significantly higher than those at other salinities.At S5,the digestive enzyme activities in intestine and stomach of rainbow trout were significantly higher than those of steelhead trout.At S10,the digestive enzyme activities in intestine and stomach of steelhead trout were significantly higher than those of rainbow trout.The activities of superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)in the liver of rainbow and steelhead trout maximized at S5 and S10 while the activities of SOD,CAT,glutathione peroxidase and MDA in the liver of steelhead trout were significantly higher than those of rainbow trout at S25 and S30.These findings indicated that juvenile rainbow and steelhead trout have the best digestion ability at S5 and S10,and the digestion and antioxidant capacities of steelhead trout were stronger than those of rainbow trout at higher salinities(25～30).
In order to compare the digestive and antioxidant abilities of juvenile rainbow and steelhead trout at different salinities,juvenile rainbow(average body weight 3.55 g)and steelhead trout(average body weight 3.57 g)were reared at 7 different salinities(0,5,10,15,20,25 and 30,abbreviated as S0,S5,S10,S15,S20,S25 and S30)for 40 d,respectively,three tanks each salinity and 8 individuals each tank.We analyzed the intestinal and gastric digestive and liver antioxidant enzyme activities of the fish individuals.The salinity was gradually adjusted with natural seawater and fresh water at a rate of1～2 d-1 and stabilized for 7 days.The results showed that the activities of intestinal amylase,lipase and trypsin and gastric amylase and pepsin in rainbow and steelhead trout peaked at S5 and S10,which were significantly higher than those at other salinities.At S5,the digestive enzyme activities in intestine and stomach of rainbow trout were significantly higher than those of steelhead trout.At S10,the digestive enzyme activities in intestine and stomach of steelhead trout were significantly higher than those of rainbow trout.The activities of superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)in the liver of rainbow and steelhead trout maximized at S5 and S10 while the activities of SOD,CAT,glutathione peroxidase and MDA in the liver of steelhead trout were significantly higher than those of rainbow trout at S25 and S30.These findings indicated that juvenile rainbow and steelhead trout have the best digestion ability at S5 and S10,and the digestion and antioxidant capacities of steelhead trout were stronger than those of rainbow trout at higher salinities(25～30).
引文
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