臭氧水对土壤微生物群落的影响
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摘要
对设施蔬菜喷洒臭氧水来探究臭氧水对土壤微生物群落的影响.以喷洒清水和百菌清农药为对照,分别以低质量浓度(3 mg·L~(-1))、中浓度(6 mg·L~(-1))和高浓度(9 mg·L~(-1))的臭氧水对土壤进行喷洒.通过Mis Seq 2×300 bp高通量测序来分析了喷洒臭氧水对土壤微生物多样性的影响.结果表明:臭氧水不会改变微生物群落结构,对组成土壤微生物中的重要的微生物群落无伤害.
Research on soil microbial biomass and diversity by spraying ozonated water of different concentrations contrast with water and chlorothalonil. We used the microbial diversity of high-throughput sequencing platform MisSeq 2 bp × 300 bp and analyzed the effects of ozonated water on soil microbial community. The results indicated that ozonated water did not change the composition of soil microbial community,and did not damage the important microbial community soil microbial which composed the soil bacteria compared with the controls.
Research on soil microbial biomass and diversity by spraying ozonated water of different concentrations contrast with water and chlorothalonil. We used the microbial diversity of high-throughput sequencing platform MisSeq 2 bp × 300 bp and analyzed the effects of ozonated water on soil microbial community. The results indicated that ozonated water did not change the composition of soil microbial community,and did not damage the important microbial community soil microbial which composed the soil bacteria compared with the controls.
引文
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[24] DIACONO M,MONTEMURRO F. Long-term effects of organic amendments on soil fertility[M]. Dordrecht:Springer,2011:761-786.
[25] NAGARAJKUMAR M,JAYARAJ J,MUTHUKRISHNAN S,et al. Detoxification of oxalic acid by Peudomonas fluorescens strain Pf MDU2:Implications for the biological control of rice sheath blight caused by Rhizoctonia solani[J]. Microbiological Research,2005,160(3):291-298.
[26]陆启环,张弢,易晓华,等.臭氧水浇灌对土壤营养成分及草莓幼苗生理特性的影响[J].北方园艺,2016(23):50-53.LU Q H,ZHANG T,YI X H,et al. Effects of ozone water irrigation on soil nutrients andphysiological characteristics of strawberry seedling[J]. Northern Horticulture,2016(23):50-53.
[2] ZHANG L,LU Z,YU Z,et al. Preservation of fresh-cut celery by treatment of ozonated water[J]. Food Control,2005,16(3):279-283.
[3] WARDLE D A,BARDGETT R D,KLIRONOMOS J N,et al. Ecological linkages between aboveground and belowground biota[J]. Science,2004,304(5677):1629-1633.
[4] ARTURSSON V,FINLAY R D,JANSSON J K. Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth[J]. Environmental Microbiology,2006,8(1):1-10.
[5] POWELL S N,SINGLETON D R,AITKEN M D. Effects of enrichment with salicylate on bacterial selection and PAH mineralization in a microbial community from a bioreactor treating contaminated soil[J]. Environmental Science and Technology,2008,42(11):4099-4105.
[6]谢红薇.熏蒸剂对土壤微生物群落的影响[D].北京:中国农业科学院,2012.XIE H W. Impact of fumigants on all soil microbial communities[D]. Beijing:Chinese Academy of Agricultural Sciences,2012.
[7]郑飞翔,王效科,侯培强,等.臭氧胁迫对水稻生长以及C、N、S元素分配的影响[J].生态学报,2011,31(6):1479-1486.ZHENGF X,WANG X K,HOU P Q,et al. Influences of elevated ozone on growth and C,N,S allocations of rice[J]. Acta Ecologica Sinica,2011,31(6):1479-1486.
[8]徐冬颖,史君彦,郑秋丽,等.臭氧处理对菠菜采后保鲜效果的影响[J].北方园艺,2018,12:125-130.XU D Y,SHI J Y,ZHENG Q L,et al. Effect of ozone on postharvest storage quality of spinach[J]. Northern Horticulture,2018,12:125-130.
[9]张瑞华,陈优明,王承香,等.不同浓度臭氧水防治根结线虫效果的研究[J].中国农学通报,2012,28(25):243-247.ZHANG R H,CHEN Y M,WANG C X,et al. Researches of ozone water controlling root-knot nematodes[J]. Chinese Agricultural Science Bulletin,2012,28(25):243-247.
[10]龙瑶.臭氧水对设施黄瓜病害防治的应用研究[D].南京:南京农业大学,2016.LONG Y. The study on application of aqueous ozone on controlling greenhouse cucumber disease[D]. Nanjing:Nanjing Agricultural University,2016.
[11]齐素敏,张思聪,庄乾营,等.臭氧和臭氧水在农业害虫防治领域的应用[J].河北农业科学,2015,19(5):36-40.QI S M,ZHANG S C,ZHUANG Q Y,et al. Applications of ozone and ozone water in controlling agricultural pests[J].Journal of Hebei Agricultural Sciences,2015,19(5):36-40.
[12] KASURINEN A,GONZALES P K,RIIKONEN J,et al. Soil CO2efflux of two silver birch clones exposed to elevated CO2and O3levels during three growing seasons[J]. Plant and Soil,1999,214(1/2):125-132.
[13] YU Y C,LIN X G,FENG Y Z,et al. Effects of elevated surface O3concentration on functional diversity of soil microbial community in rice field[J]. Acta Pedologica Sinica,2011,48(6):1227-1234.
[14] BAKER G C,SMITH J J,COWAN D A. Review and re-analysis of domain-specific 16S primers[J]. Journal of Microbiological Methods,2003,55(3):541-555.
[15] LIU Z,DESANTIS T Z,ANDERSEN G L,et al. Accurate taxonomy assignments from 16S rRNA sequences produced by highly parallel pyrosequencers[J]. Nucleic Acids Research,2008,36:e120.
[16]夏文旭.酿酒葡萄根际土壤肥力与微生物多样性及生理活性研究[D].兰州:兰州理工大学,2016.XIA W X. Research on soil fertility and microbial diversity and physiological activities of the Rhizosphere of Vitis vinifera[D]. Lanzhou:Lanzhou University of Technology,2016.
[17] WANG Y,QIAN P Y. Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies[J]. PLo S One,2009,4:e7401.
[18]陈展,王效科,段晓男,等.臭氧浓度升高对盆栽小麦根系和土壤微生物功能的影响[J].生态学报,2007,27(5):1803-1808.CHEN Z,WANG X K,DUAN X N,et al. Ozone effects on wheat root and soil microbial biomass and diversity[J]. Acta Ecologica Sinica,2007,27(5):1803-1808.
[19]赵燕,薛林贵,李琳,等.丛毛单胞菌在环境污染物降解方面的研究进展[J].微生物学通报,2012,39(10):1471-1478.ZHAO Y,XUE L G,LI L,et al. Advance in environmental pollutants degradation of comamonas[J]. Microbiology China,2012,39(10):1471-1478.
[20] ANDERSON C R,CONDRON L M,CLOUGH T J,et al. Biochar induced soil microbial community change:Implications for biogeochemical cycling of carbon,nitrogen and phosphorus[J]. Pedobiologia,2011,54(5/6):309-320.
[21] MALIK Z A,AHMED S. Degradation of petroleum hydrocarbons by oil field isolated consortium bacterial[J]. African Journal of Biotechnology,2012,11(3):650-658.
[22] PRUESSE E,QUAST C,KNITTEL K,et al. SILVA:A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB[J]. Nucleic Acids Research,2007,35:7188-7196.
[23]徐瑞富,王小龙.花生连作田土壤微生物群落动态与土壤养分关系研究[J].花生学报,2003,32(3):19-24.XU R F,WANG X L. Relation of microbial population dynamics and nutrient in soil of continuous cropping with peanut[J]. Journal of Peanut Science,2003,32(3):19-24.
[24] DIACONO M,MONTEMURRO F. Long-term effects of organic amendments on soil fertility[M]. Dordrecht:Springer,2011:761-786.
[25] NAGARAJKUMAR M,JAYARAJ J,MUTHUKRISHNAN S,et al. Detoxification of oxalic acid by Peudomonas fluorescens strain Pf MDU2:Implications for the biological control of rice sheath blight caused by Rhizoctonia solani[J]. Microbiological Research,2005,160(3):291-298.
[26]陆启环,张弢,易晓华,等.臭氧水浇灌对土壤营养成分及草莓幼苗生理特性的影响[J].北方园艺,2016(23):50-53.LU Q H,ZHANG T,YI X H,et al. Effects of ozone water irrigation on soil nutrients andphysiological characteristics of strawberry seedling[J]. Northern Horticulture,2016(23):50-53.