一测多评测定玉叶金花中4种有机酸的含量
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摘要
建立玉叶金花提取物中有机酸类成分含量测定的一测多评方法,探讨一测多评法在中药材质量控制中的应用.以绿原酸(3-咖啡酰奎宁酸)为内标物,分别建立异绿原酸A(3,5-二咖啡酰奎宁酸)、异绿原酸B(3,4-二咖啡酰奎宁酸)、异绿原酸C(4,5-二咖啡酰奎宁酸)的相对校正因子,通过外标法和一测多评法2种不同的方法测定计算4种有机酸的含量,并比较不同方法结果的相对误差.利用一测多评法和外标法测定广西6个不同产地的4种有机酸类成分结果无显著差异.一测多评可用于玉叶金花中4种有机酸类成分的测定,且具良好的可行性,同时具有较高的经济性.
A method of quantitative analysis of multi-components by single-marker for the determination of organic acid content in the extract of Mussaenda pubescens is established,the application of the method for the quality control of the traditional Chinese medicine is discussed.Chlorogenic acid(3-caffeoylquinic acid),was used as the internal standard to establish the relative correction factors of isochlorogenic acid A(3,5-O-di-caffeoylquinic acid),isochlorogenic acid B(3,4-O-di-caffeoylquinic acid),and isochlorogenic acid C(4,5-O-di-caffeoylquinic acid),respectively.The external standard method and QAMS method were used to calculate four kinds of relative correction factors.The content of organic acids were determined and the relative error of the results of the two methods was compared.There is no significant difference between the two methods for the determination of four organic acid components in six different producing areas in Guangxi.The method of QAMS can be used to determine the content of four organic acids in Mussaenda Pubescens,and it shows good feasibility and high economic efficiency.
A method of quantitative analysis of multi-components by single-marker for the determination of organic acid content in the extract of Mussaenda pubescens is established,the application of the method for the quality control of the traditional Chinese medicine is discussed.Chlorogenic acid(3-caffeoylquinic acid),was used as the internal standard to establish the relative correction factors of isochlorogenic acid A(3,5-O-di-caffeoylquinic acid),isochlorogenic acid B(3,4-O-di-caffeoylquinic acid),and isochlorogenic acid C(4,5-O-di-caffeoylquinic acid),respectively.The external standard method and QAMS method were used to calculate four kinds of relative correction factors.The content of organic acids were determined and the relative error of the results of the two methods was compared.There is no significant difference between the two methods for the determination of four organic acid components in six different producing areas in Guangxi.The method of QAMS can be used to determine the content of four organic acids in Mussaenda Pubescens,and it shows good feasibility and high economic efficiency.
引文
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[20]于晓,戴衍朋,周倩,等.一测多评法测定艾叶中6个有机酸类成分的含量[J].药物分析杂志,2016,36(12):2131-2138.
[2]邹节明主编.广西特色中草药资源选编[M].北京:科学出版社,2011:665-667.
[3]韦奇志,周智,陈邦树,等.玉叶清火片药效学试验[J].广东药学院学报,2003,19(1):32-34.
[4]刘丽娟,刘妮,史晨希,等.玉叶解毒颗粒对多种呼吸道病毒作用体外实验研究[J].中国热带医学,2010,10(7):806-807.
[5]王霞,杨健,宋菲,等.苗药黑骨藤中咖啡酰基奎宁酸类部位对人类风湿性关节炎成纤维样滑膜细胞MH7A增殖及炎症因子分泌的影响[J].中国药房,2017,28(28):3949-3952.
[6]MOTAAL A A,EZZAT S M,TADROS M G,et al.anti-inflammatory activity of caffeoylquinic acid derivatives from in rats[J].Pharmaceutical Biology,2016:1-7.
[7]朱乃亮,彭平,赵丽敏,等.植物中常见咖啡酰奎宁酸类化合物研究进展[C].中华中医药学会中药化学分会学术年会.2013.
[8]BAJKO E,KALINOWSKA M,BOROWSKI P,et al.5-O-caffeoylquinic acid:A spectroscopic study and biological screening for antimicrobial activity[J].LWT-Food Science and Technology,2016,65(1):471-479.
[9]李佳银,于欢,石伯阳,等.甘薯茎叶中异槲皮苷及咖啡酰基奎宁酸类衍生物的抗氧化活性[J].食品科学,2013,34(7):111-114.
[10]LI X,LI K,XIE H,et al.Antioxidant and cytoprotective effects of the Di-O-caffeoylquinic acid family:the mechanism,structure-activity relationship,and conformational Effect[J].Molecules,2018,23(1):222.
[11]LIU L,LI Y.Chondroprotective and anti-nociceptive effects of caffeoylquinic acid in osteoarthritis by downregulating catabolic activity and oxidative damage in chondrocytes[J].Biomedicine&Pharmacotherapy,2017,93:985-994.
[12]夏超,李满妹,李药兰,等.天然咖啡酰基奎宁酸类化合物抗RSV活性及其构效关系研究[J].中药材,2016,39(2):383-388.
[13]陈建维,刘圆,刘晟楠,等.一测多评法测定枳实中4种黄酮类成分[J].中草药,2015,46(09):1374-1377.
[14]李丹,李会军,高雯,等.一测多评法测定山银花提取物中6种绿原酸类成分[J].中国中药杂志,2014,39(12):2305-2310.
[15]FENG W H,LI C,JI L N,et al.Quantitative analysis of nine ginsenosides in red ginseng using QAMS method based on high-resolution and reference chromatography of red ginseng[J].China Journal of Chinese Materia Medica,2016,41(18):3389.
[16]张初瑜,陈素红,吴素香.一测多评法测定复方人参片中的8种苷类成分[J].中国现代应用药学,2018,35(05):708-714.
[17]何兵,刘艳,杨世艳,等.HPLC一测多评法同时测定双青咽喉片中10种成分[J].中草药,2013,44(08):974-981.
[18]CUI L,ZHANG Y,SHAO W,et al.Analysis of the HPLC fingerprint and QAMS from Pyrrosia species[J].Industrial Crops&Products,2016,85:29-37.
[19]ANDREY S,ELENA S,ANNA P,et al.Combination of HPLC-MS and QAMS as a new analytical approach for determination of saponins in ginseng containing products[J].Journal of Pharmaceutical&Biomedical Analysis,2017,132:87-92.
[20]于晓,戴衍朋,周倩,等.一测多评法测定艾叶中6个有机酸类成分的含量[J].药物分析杂志,2016,36(12):2131-2138.