饲粮中添加超氧化物歧化酶模拟物对肉仔鸡肌纤维特性及肌肉超氧化物歧化酶活性的影响
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摘要
本试验旨在研究饲粮中添加超氧化物歧化酶模拟物(SODm)对肉仔鸡肌纤维特性及肌肉超氧化物歧化酶(SOD)活性的影响。试验选取1日龄爱拔益加(AA)肉仔鸡360只,随机分为6组,每组6个重复,每个重复10只鸡。各组分别饲喂SODm水平为0(对照)、1.0‰、1.5‰、2.0‰、2.5‰、3.0‰的试验饲粮,试验期为42 d。结果表明,各组肉仔鸡生长性能指标无显著差异(P>0.05)。1.0‰SODm组肉仔鸡胸肌纤维密度显著高于其他各组(P<0.05),1.5‰SODm组肉仔鸡胸肌纤维直径显著高于除2.0‰SODm组外的其他各组(P<0.05)。1.5‰SODm组肉仔鸡腿肌肌纤维密度显著高于其他各组(P<0.05),1.5‰SODm组肉仔鸡腿肌肌纤维直径显著低于其他各组(P<0.05)。2.0‰SODm组肉仔鸡胸肌剪切力显著低于对照组(P<0.05)。3.0‰SODm组肉仔鸡胸肌SOD活性显著高于其他各组(P<0.05),1.5‰SODm组肉仔鸡腿肌SOD活性显著高于除3.0‰SODm组外的其他各组(P<0.05)。由此可知,饲粮中添加SODm可以改善肉仔鸡肌纤维特性,提高肌肉中SOD的活性,在一定程度上改善肉品质,同时增强肌肉的抗氧化能力。
The aim of this study was to investigate the effects of dietary superoxide dismutase simulant( SODm) on myofiber characteristics and muscle superoxide dismutase( SOD) activity of broilers. A total of360 one-day-old Arbor Acres( AA) broilers were randomly divided into 6 groups with 6 replicates in each group and 10 chickens in each replicate. The broilers in 6 groups were fed the experimental diets with 0( control),1.0‰,1. 5‰,2. 0‰,2. 5‰ and 3. 0‰ SODm,respectively. The experimental period was 42 days.The results showed that there were no significant differences in growth performance indexes of broilers among all groups( P>0.05). The breast muscle fiber density of broilers in 1.0‰ SODm group was significantly higher than that in other groups( P<0.05),the breast muscle fiber diameter of broilers in 1.5‰ SODm group was significantly higher than that in other groups except 2.0‰ SODm group( P<0.05). The leg muscle fiber density of broilers in 1.5‰ SODm group was significantly higher than that in other groups( P<0.05),the leg muscle fiber diameter of broilers in 1. 5‰ SODm group was significantly lower than that in other groups( P <0.05). The leg muscle shear force of broilers in 2.0‰ SODm group was significantly lower than that in control group( P<0.05). The breast muscle SOD activity of broilers in 3.0‰ SODm group was significantly higher than that in other groups( P<0.05),the leg muscle SOD activity of broilers in 1.5‰ SODm group was significanly higher than that in other groups except 3.0‰ SODm group( P<0.05). It is concluded that the addition of SODm in the diet can improve the myofiber characteristics of broilers,improve the muscle SOD activity,improve the meat quality to a certain extent,while enhance the muscle antioxidant ability.
The aim of this study was to investigate the effects of dietary superoxide dismutase simulant( SODm) on myofiber characteristics and muscle superoxide dismutase( SOD) activity of broilers. A total of360 one-day-old Arbor Acres( AA) broilers were randomly divided into 6 groups with 6 replicates in each group and 10 chickens in each replicate. The broilers in 6 groups were fed the experimental diets with 0( control),1.0‰,1. 5‰,2. 0‰,2. 5‰ and 3. 0‰ SODm,respectively. The experimental period was 42 days.The results showed that there were no significant differences in growth performance indexes of broilers among all groups( P>0.05). The breast muscle fiber density of broilers in 1.0‰ SODm group was significantly higher than that in other groups( P<0.05),the breast muscle fiber diameter of broilers in 1.5‰ SODm group was significantly higher than that in other groups except 2.0‰ SODm group( P<0.05). The leg muscle fiber density of broilers in 1.5‰ SODm group was significantly higher than that in other groups( P<0.05),the leg muscle fiber diameter of broilers in 1. 5‰ SODm group was significantly lower than that in other groups( P <0.05). The leg muscle shear force of broilers in 2.0‰ SODm group was significantly lower than that in control group( P<0.05). The breast muscle SOD activity of broilers in 3.0‰ SODm group was significantly higher than that in other groups( P<0.05),the leg muscle SOD activity of broilers in 1.5‰ SODm group was significanly higher than that in other groups except 3.0‰ SODm group( P<0.05). It is concluded that the addition of SODm in the diet can improve the myofiber characteristics of broilers,improve the muscle SOD activity,improve the meat quality to a certain extent,while enhance the muscle antioxidant ability.
引文
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[2]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技报,2013,15(5):53-58.
[3]郝奎,王世喜,张淑兰等.SODm叶面肥在甜菜上的应用效果[J].中国糖料,2011,4:54-55.
[4]章法源.SODm尿素在东乡县玉米上的肥效试验示范总结[J].农业科技与信息,2015,6:42-44.
[5]叶锋,苏桂华.SODm尿素在玉米上的肥效试验初报[J].新疆农垦科技,2016,3:55-56.
[6]曹健,张珊珊.SODm尿素在棉花上的示范[J].农村科技,2014,4:22-23
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[9]陈洁波,陶林,吴薇薇,等.不同品种优质鸡肌纤维特性与肉品质关系的研究[J].中国家禽,2013,35(16):12-15.
[10]MA X Y,JIANG Z Y,LIN Y C,et al.Dietary supplementation with carnosine improves antioxidant capacity and meat quality of finishing pigs[J].Journal of Animal Physiology and Animal Nutrition,2010,94:286-295.
[11]ZHONG R Z,TAN C Y,HAN X F,et al.Effect of dietary tea catechins supplementation in goats on the quality of meat kept under refrigeration[J].Small Ruminant Research,2009,87(1/2 3):122-125.
[12]ZHONG R Z,ZHOU D W,TAN C Y,et al.Effect of tea catechins on regulation of antioxidant enzyme expression in H2O2-induced skeletal muscle cells of goat in vitro[J].Journal of Agricultural and Food Chemistry,2011,59(20):11338-11343.
[13]HAMANO Y,SUGAWARA S,KAMOTA Y.et al.Involvement of lipoic acid in plasma metabolites,hepatic oxygen consumption,and metabolic response to a betaagonist in broiler chickens[J].British Journal of Nutrition,1999.82:497-503.
[14]张勇.硫辛酸、乙酰肉碱对肉仔鸡肉品质及肌纤维类型分布的营养调控[D].博士学位论文.北京:中国农业大学.2010.
[15]邹杨,杨在宾,杨维仁,等.不同抗氧化剂和添加水平对肉仔鸡生产性能和抗氧化性影响的研究[J].家禽科学,2011,8:7-10.
[16]翁茁先,李威娜,钟鸣,等.五华三黄鸡不同生态养殖模式效果评价[J].饲料研究,2016,12:1-5,45.
[17]苏琳,辛雪,刘树军,等.苏尼特羊肉肌纤维特性与肉质相关性研究[J].食品科学,2014,35(7):7-11.
[18]张崇志,高爱武,侯先志,等.不同营养水平对羔羊肌肉组织学性状的影响[J].动物营养学报,2011,23(2):336-342.
[19]SCHIAFFINO S,GORZA L,SARTORE S,et al.Three myosin heavy chain isoforms in type 2 skeletal muscle fibres[J].Journal of Muscle Research and Cell Motility,1989,10:197-205.
[20]LAFRAMBOISE W A,DAOOD M J,GUTHRIE R D,et al.Electrophoretic separation and immunological identification of type 2X myosin heavy chain in rat skeletal muscle[J].Biochimica et Biophysica Acta,1990,1035:109-112.
[21]李伯江,李平华,吴望军,等.骨骼肌肌纤维形成机制的研究进展[J].中国农业科学,2014,47(6):1200-1207.
[22]WU Z,PUIGSERVERP P,ANDERSSON U,et al.Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1[J].Cell,1999,98:115-124.
[23]LIN J,WU H,TARRY B,et al.Transcriptional co-activator PGC-1αdrives the formation of slow-twitch muscle fibers[J].Nature,2002,418:797-801.
[24]SCHULER M,ALI F,CHAMBON C,et al.PGC1 alpha expression is controlled in skeletal muscles by PPAR beta,whose ablation results in fiber-type switching,o besity,and type 2 diabetes[J].Cell Metabolism,2006,4(5):407-414.
[25]方海琴,张勇,许佑君,等.PPARδ激动剂GW501516调节小鼠骨骼肌线粒体生物合成和纤维类型转换的作用[J].中国药理学通报,2010,26(10):1290-1295.
[26]刘冰,杨君,杨宁.不同品种鸡肌纤维的发育规律及杂种优势研究[J].畜牧兽医学报,2006,37(8):829-833.
[27]韩海霞,曹顶国,雷秋霞,等.日粮营养水平对鲁禽3号麻鸡肌纤维直径和密度的影响[J].饲料研究,2008(1):47-49.
[28]杨敏馨,寇涛,李悦,等.解淀粉芽孢杆菌ES-2对肉鸡屠宰性能和肉品质及肌肉抗氧化能力的影响[J].南京农业大学学报,2016,39(2):255-261.
[29]蒋雪樱,张相伦,陆鹏,等.蛋氨酸对肉鸡屠宰性能、肉品质及肌肉抗氧化的影响[J].食品科学,2016,37(21):114-118.
[30]CHAN K M,DECKER E A,FEUSTMAN C.Endogenous skeletal muscle antioxidants[J].Critical Reviews in Food Science and Nutrition,1994,34(4):403-426.
[31]SATOSHI S,KIYOJII T,HIROVO K,et al.Exerciseinduced lipid peroxidation and leakage of enzymes before and after vitamin E supplementation[J].International Journal of Biochemistry,1989,21(8):835-838.
[2]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技报,2013,15(5):53-58.
[3]郝奎,王世喜,张淑兰等.SODm叶面肥在甜菜上的应用效果[J].中国糖料,2011,4:54-55.
[4]章法源.SODm尿素在东乡县玉米上的肥效试验示范总结[J].农业科技与信息,2015,6:42-44.
[5]叶锋,苏桂华.SODm尿素在玉米上的肥效试验初报[J].新疆农垦科技,2016,3:55-56.
[6]曹健,张珊珊.SODm尿素在棉花上的示范[J].农村科技,2014,4:22-23
[7]MALTIN C,BALEERZKA D,TILLEY R,et al.Detemrinants of meat quality:tenderness[J].The Proceedings of the Nutrition Society,2003,62(2):337-347.
[8]陈宽维,李慧芳,张学余,等.肉鸡肌纤维与肉质关系研究[J].中国畜牧杂志,2002,38(6):6-7.
[9]陈洁波,陶林,吴薇薇,等.不同品种优质鸡肌纤维特性与肉品质关系的研究[J].中国家禽,2013,35(16):12-15.
[10]MA X Y,JIANG Z Y,LIN Y C,et al.Dietary supplementation with carnosine improves antioxidant capacity and meat quality of finishing pigs[J].Journal of Animal Physiology and Animal Nutrition,2010,94:286-295.
[11]ZHONG R Z,TAN C Y,HAN X F,et al.Effect of dietary tea catechins supplementation in goats on the quality of meat kept under refrigeration[J].Small Ruminant Research,2009,87(1/2 3):122-125.
[12]ZHONG R Z,ZHOU D W,TAN C Y,et al.Effect of tea catechins on regulation of antioxidant enzyme expression in H2O2-induced skeletal muscle cells of goat in vitro[J].Journal of Agricultural and Food Chemistry,2011,59(20):11338-11343.
[13]HAMANO Y,SUGAWARA S,KAMOTA Y.et al.Involvement of lipoic acid in plasma metabolites,hepatic oxygen consumption,and metabolic response to a betaagonist in broiler chickens[J].British Journal of Nutrition,1999.82:497-503.
[14]张勇.硫辛酸、乙酰肉碱对肉仔鸡肉品质及肌纤维类型分布的营养调控[D].博士学位论文.北京:中国农业大学.2010.
[15]邹杨,杨在宾,杨维仁,等.不同抗氧化剂和添加水平对肉仔鸡生产性能和抗氧化性影响的研究[J].家禽科学,2011,8:7-10.
[16]翁茁先,李威娜,钟鸣,等.五华三黄鸡不同生态养殖模式效果评价[J].饲料研究,2016,12:1-5,45.
[17]苏琳,辛雪,刘树军,等.苏尼特羊肉肌纤维特性与肉质相关性研究[J].食品科学,2014,35(7):7-11.
[18]张崇志,高爱武,侯先志,等.不同营养水平对羔羊肌肉组织学性状的影响[J].动物营养学报,2011,23(2):336-342.
[19]SCHIAFFINO S,GORZA L,SARTORE S,et al.Three myosin heavy chain isoforms in type 2 skeletal muscle fibres[J].Journal of Muscle Research and Cell Motility,1989,10:197-205.
[20]LAFRAMBOISE W A,DAOOD M J,GUTHRIE R D,et al.Electrophoretic separation and immunological identification of type 2X myosin heavy chain in rat skeletal muscle[J].Biochimica et Biophysica Acta,1990,1035:109-112.
[21]李伯江,李平华,吴望军,等.骨骼肌肌纤维形成机制的研究进展[J].中国农业科学,2014,47(6):1200-1207.
[22]WU Z,PUIGSERVERP P,ANDERSSON U,et al.Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1[J].Cell,1999,98:115-124.
[23]LIN J,WU H,TARRY B,et al.Transcriptional co-activator PGC-1αdrives the formation of slow-twitch muscle fibers[J].Nature,2002,418:797-801.
[24]SCHULER M,ALI F,CHAMBON C,et al.PGC1 alpha expression is controlled in skeletal muscles by PPAR beta,whose ablation results in fiber-type switching,o besity,and type 2 diabetes[J].Cell Metabolism,2006,4(5):407-414.
[25]方海琴,张勇,许佑君,等.PPARδ激动剂GW501516调节小鼠骨骼肌线粒体生物合成和纤维类型转换的作用[J].中国药理学通报,2010,26(10):1290-1295.
[26]刘冰,杨君,杨宁.不同品种鸡肌纤维的发育规律及杂种优势研究[J].畜牧兽医学报,2006,37(8):829-833.
[27]韩海霞,曹顶国,雷秋霞,等.日粮营养水平对鲁禽3号麻鸡肌纤维直径和密度的影响[J].饲料研究,2008(1):47-49.
[28]杨敏馨,寇涛,李悦,等.解淀粉芽孢杆菌ES-2对肉鸡屠宰性能和肉品质及肌肉抗氧化能力的影响[J].南京农业大学学报,2016,39(2):255-261.
[29]蒋雪樱,张相伦,陆鹏,等.蛋氨酸对肉鸡屠宰性能、肉品质及肌肉抗氧化的影响[J].食品科学,2016,37(21):114-118.
[30]CHAN K M,DECKER E A,FEUSTMAN C.Endogenous skeletal muscle antioxidants[J].Critical Reviews in Food Science and Nutrition,1994,34(4):403-426.
[31]SATOSHI S,KIYOJII T,HIROVO K,et al.Exerciseinduced lipid peroxidation and leakage of enzymes before and after vitamin E supplementation[J].International Journal of Biochemistry,1989,21(8):835-838.