仔猪红黄痢疫苗的最小免疫剂量、免疫持续期和保存期试验
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摘要
用大肠杆菌K88、K99、987P菌株和C型产气荚膜梭菌制备成仔猪大肠杆菌病、C型产气荚膜梭菌病二联灭活疫苗(仔猪红黄痢疫苗),以不同剂量的疫苗免疫怀孕母猪,然后对其所产仔猪用发病剂量的相应菌株进行攻毒,同时将连续3批疫苗置2～8℃保存,分别于保存后的不同时间取样进行保存期试验。结果发现,用1.6mL以上剂量疫苗免疫组仔猪的攻毒保护率均在80%以上。通过对免疫母猪所产的1、4、7和10日龄仔猪进行攻毒,仔猪的攻毒保护率均不低于80%,说明疫苗免疫产生期为仔猪吸食初乳后24h内,仔猪被动免疫持续期为7d以上。疫苗保存期检测结果显示,疫苗保存27个月后,疫苗的性状检验合格,对怀孕母猪安全,疫苗免疫母猪所产仔猪攻毒保护率均不低于80%。因此,确定疫苗的最小免疫剂量为1.6mL,仔猪被动免疫持续期为7d以上,疫苗保存期为2～8℃保存24个月。
The Escherichia coli K88,K99,987 Pstrains and C type Clostridium perfringens were used to prepare piglets colibacillosis.Pregnant sows were immunized with different doses of vaccine,and then their piglets were challenged with the corresponding pathogenic strains and doses.The results showed that the protection rate of the piglets was above 80% when the vaccine doses was 1.6 mL or more.The protection rate of piglets against bacteria was not less than 80% when piglets aged 1,4,7 and 10 dwere challenged,which indicated that the immunization period was within 24 hafter colostrum feeding and the duration of passive immunization was more than 7 d.Three batches of vaccines were kept at 2-8℃and tested at different times during preservation.The results showed that the vaccine was safe for pregnant sows after 27 months of preservation and the protective rate of piglets produced by the vaccine immunized sows was no less than 80%.Therefore,the minimum immune dose of the vaccine was 1.6 mL,the passive immunization duration of piglets was more than 7 d,and the vaccine storage period was 24 months at 2-8℃.
The Escherichia coli K88,K99,987 Pstrains and C type Clostridium perfringens were used to prepare piglets colibacillosis.Pregnant sows were immunized with different doses of vaccine,and then their piglets were challenged with the corresponding pathogenic strains and doses.The results showed that the protection rate of the piglets was above 80% when the vaccine doses was 1.6 mL or more.The protection rate of piglets against bacteria was not less than 80% when piglets aged 1,4,7 and 10 dwere challenged,which indicated that the immunization period was within 24 hafter colostrum feeding and the duration of passive immunization was more than 7 d.Three batches of vaccines were kept at 2-8℃and tested at different times during preservation.The results showed that the vaccine was safe for pregnant sows after 27 months of preservation and the protective rate of piglets produced by the vaccine immunized sows was no less than 80%.Therefore,the minimum immune dose of the vaccine was 1.6 mL,the passive immunization duration of piglets was more than 7 d,and the vaccine storage period was 24 months at 2-8℃.
引文
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[5]房海.大肠埃希氏菌[M].石家庄:河北科学技术出版社,1997.
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