丙泊酚通过下调BNIPL-2抑制结肠癌增殖的研究
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摘要
目的观察丙泊酚在结肠癌细胞中对BNIPL-2的作用,并探讨其机制。方法应用TCGA数据库对结直肠癌患者进行数据分析,通过CCK8实验,qRT-PCR阐明了丙泊酚对细胞活力和致肿瘤性的影响。结果 BNIPL-2在结肠癌组织中大量表达,并与预后不良、恶性阶段有关。BNIPL-2促进CRC细胞系SW480的增殖。丙泊酚抑制结肠癌细胞的增殖作用,并且下调SW480结肠癌细胞中BNIPL-2的表达。结论丙泊酚通过下调BNIPL-2抑制结肠癌增殖,这可以促进更好地理解丙泊酚在临床环境中的使用。
Objective To observe the effect of propofol on BNIPL-2 in colon cancer cells and to investigate the mechanism. Methods The TCGA database was utilized to analyze the colorectal cancer patients. The effect of propofol on cell viability and tumorigenicity was demonstrated by CRT8 assay and qRT-PCR. Results BNIPL-2 was highly expressed in colon cancer tissues and was associated with a poor prognosis and malignant stage. BNIPL-2 promoted the proliferation of CRC. Phenol inhibits the proliferation of colon cancer cells and down-regulates the expression of BNIPL-2 in SW480 colon cancer cells.Conclusion Propofol inhibits colon cancer proliferation by down-regulating BNIPL-2, which promotes better understanding of the use of propofol in clinical.
Objective To observe the effect of propofol on BNIPL-2 in colon cancer cells and to investigate the mechanism. Methods The TCGA database was utilized to analyze the colorectal cancer patients. The effect of propofol on cell viability and tumorigenicity was demonstrated by CRT8 assay and qRT-PCR. Results BNIPL-2 was highly expressed in colon cancer tissues and was associated with a poor prognosis and malignant stage. BNIPL-2 promoted the proliferation of CRC. Phenol inhibits the proliferation of colon cancer cells and down-regulates the expression of BNIPL-2 in SW480 colon cancer cells.Conclusion Propofol inhibits colon cancer proliferation by down-regulating BNIPL-2, which promotes better understanding of the use of propofol in clinical.
引文
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[15]Siddiqui RA,Zerouga M,Wu M,et al.Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells[J].Breast Cancer Research,2005,7(5):R645-R654.
[16]Wang ZT,Gong HY,Zheng F,et al.Propofol suppresses proliferation and invasion of pancreatic cancer cells by upregulating microRNA-133a expression[J].Genetics&Molecular Research Gmr,2015,14(3):7529-7537.
[17]Xu YJ,Li SY,Cheng Q,et al.Effects of anaesthesia on proliferation,invasion and apoptosis of LoVo colon cancer cells in vitro[J].Anaesthesia,2015,71(2):147-154.
[18]Peng Z,Zhang Y.Propofol inhibits proliferation and accelerates apoptosis of human gastric cancer cells by regulation of microRNA-451 and MMP-2 expression[J].Genetics and Molecular Research,2016,15(2).doi:10.4238/gmr.15027078.
[19]Chen X,Wu Q,You L,et al.Propofol attenuates pancreatic cancer malignant potential via inhibition of NMDA receptor[J].European Journal of Pharmacology,2017,795:150-159.
[20]Chen X,Lu P,Chen L,et al.Perioperative propofol-paravertebral anesthesia decreases the metastasis and progression of breast cancer[J].Tumor Biology,2015,36(11):8259-8266.
[21]Zhang D,Zhou XH,Zhang J,et al.Propofol promotes cell apoptosis via inhibiting HOTAIR mediated mTOR pathway in cervical cancer[J].Biochemical and Biophysical Research Communications,2015,468(4):561-567.
[2]Arnold M,Sierra MS,Laversanne M,et al.Global patterns and trends in colorectal cancer incidence and mortality[J].Gut,2016,66(4):683-691.
[3]Zhai Z,Yu X,Yang B,et al.Colorectal Cancer Heterogeneity and Targeted Therapy:Clinical Implications,Challenges and Solutions for Treatment Resistance[J].Seminars in Cell&Developmental Biology,2017,64:107-115.
[4]Qin W,Hu J,Guo M,et al.BNIPL-2,a novel homologue of BNIP-2,interacts with Bcl-2 and Cdc42GAP in apoptosis[J].Biochemical&Biophysical Research Communications,2003,308(2):379-385.
[5]Etienne-Manneville S,Hall A.Rho GTPases in cell biology[J].Nature,2002,420(6916):629-635.
[6]Shang X,Zhou YT,Low BC.Concerted Regulation of Cell Dynamics by BNIP-2 and Cdc42GAP Homology/Sec14p-like,Proline-rich,and GTPase-activating Protein Domains of a Novel Rho GTPase-activating Protein,BPGAP1[J].Journal of Biological Chemistry,2003,278(46):45903-45914.
[7]Ciechanowicz SJ,Ma D.Anaesthesia for oncological surgery-can it really influence cancer recurrence?[J].Anaesthesia,2016,71(2):127-131.
[8]Ecimovic P,Murray D,Doran P,et al.Propofol and Bupivacaine in Breast Cancer Cell Function In Vitro-Role of the NET1 Gene[J].Anticancer Research,2014,34(3):1321-1331.
[9]Mathyhartert M,Debydupont G,Hans P,et al.Protective activity of propofol,Diprivan and intralipid against active oxygen species[J].Mediators Inflamm,1998,7(5):327-333.
[10]Chidambaran V,Costandi A,D′Mello A.Propofol:A Review of its Role in Pediatric Anesthesia and Sedation[J].CNS Drugs,2015,29(7):543-563.
[11]Bajwa SJS,Bajwa SK,Kaur J.Comparison of two drug combinations in total intravenous anesthesia:Propofol-ketamine and propofolfentanyl[J].Saudi Journal of Anaesthesia,2010,4(2):72-79.
[12]Kiriyama S,Naitoh H,Kuwano H.Propofol sedation during endoscopic treatment for early gastric cancer compared to midazolam[J].World Journal of Gastroenterology Wjg,2014,20(34):11985-11989.
[13]Shravah J,Wang B,Pavlovic M,et al.Propofol mediates signal transducer and activator of transcription 3 activation and crosstalk with phosphoinositide 3-kinase/AKT[J].JAK-STAT,2014,3(2):e29554.
[14]Kurt M,Bilge SS,Kukula O,et al.Anxiolytic-like profile of propofol,a general anesthetic,in the plus-maze test in mice[J].Polish Journal of Pharmacology,2003,55(6):973-977.
[15]Siddiqui RA,Zerouga M,Wu M,et al.Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells[J].Breast Cancer Research,2005,7(5):R645-R654.
[16]Wang ZT,Gong HY,Zheng F,et al.Propofol suppresses proliferation and invasion of pancreatic cancer cells by upregulating microRNA-133a expression[J].Genetics&Molecular Research Gmr,2015,14(3):7529-7537.
[17]Xu YJ,Li SY,Cheng Q,et al.Effects of anaesthesia on proliferation,invasion and apoptosis of LoVo colon cancer cells in vitro[J].Anaesthesia,2015,71(2):147-154.
[18]Peng Z,Zhang Y.Propofol inhibits proliferation and accelerates apoptosis of human gastric cancer cells by regulation of microRNA-451 and MMP-2 expression[J].Genetics and Molecular Research,2016,15(2).doi:10.4238/gmr.15027078.
[19]Chen X,Wu Q,You L,et al.Propofol attenuates pancreatic cancer malignant potential via inhibition of NMDA receptor[J].European Journal of Pharmacology,2017,795:150-159.
[20]Chen X,Lu P,Chen L,et al.Perioperative propofol-paravertebral anesthesia decreases the metastasis and progression of breast cancer[J].Tumor Biology,2015,36(11):8259-8266.
[21]Zhang D,Zhou XH,Zhang J,et al.Propofol promotes cell apoptosis via inhibiting HOTAIR mediated mTOR pathway in cervical cancer[J].Biochemical and Biophysical Research Communications,2015,468(4):561-567.