金梅清暑颗粒的定性定量方法研究
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摘要
目的建立金梅清暑颗粒的定性定量方法。方法采用TLC法对金梅清暑颗粒中的山银花进行定性鉴别;采用HPLC法对金梅清暑颗粒中的绿原酸进行含量测定。结果薄层鉴别斑点清晰,分离度较好,阴性无干扰。含量测定精密度、稳定性、重复性良好,平均回收率为104.56%,RSD值为2.53%。结论该方法简便,结果准确可靠,可作为其质量控制方法。
Objective To establish the qualitative and quantitative methods of Jinmeiqingshu Granules.Methods TLC identification method was used for Lonicerae Flos in Jinmeiqingshu Granules;HPLC was applied to the determination of chlorogenic acid.Results The spots of TLC were clear with good resolution.The method had good precision,stability and reproducibility,and the mean recovery for chlorogenic acid was 104.56%(RSD=2.53%).Conclusion This study has proposed a rapid,accurate and simple method which could be used for the quality control of Jinmeiqingshu Granules.
Objective To establish the qualitative and quantitative methods of Jinmeiqingshu Granules.Methods TLC identification method was used for Lonicerae Flos in Jinmeiqingshu Granules;HPLC was applied to the determination of chlorogenic acid.Results The spots of TLC were clear with good resolution.The method had good precision,stability and reproducibility,and the mean recovery for chlorogenic acid was 104.56%(RSD=2.53%).Conclusion This study has proposed a rapid,accurate and simple method which could be used for the quality control of Jinmeiqingshu Granules.
引文
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[19]孙玲,樊晓兰,郭绮,等.一测多评法同时测定山银花药材中5种皂苷类成分的含量[J].中国药房,2017,28(18):2546-2549.
[20]王浩兵,邓力,马元春,等.基于UHPLC的金银花和山银花的鉴别及测定研究[J].中草药,2017,48(12):2516-2521.
[2]林洁,李志浩.HPLC测定金梅清暑颗粒中绿原酸与木犀草苷的含量[J].国际中医中药杂志,2016,38(2):159-162.
[3]丁野,马杰,童珂,等.忍冬和灰毡毛忍冬的色谱鉴别方法研究[J].中南药学,2011,9(10):725-728.
[4]周送桂,何珺,肖序,等.HPLC法监测金英黄归汤制备过程中绿原酸和连翘酯苷A含量[J].中药新药与临床药理,2015,26(1):109-112.
[5]孙健,陆彦,吴国娟.绿原酸注射剂的稳定性试验[J].黑龙江畜牧兽医:科技版,2013,(5):127-129.
[6]李建晨,廖明丽,贾玉捷,等.双黄连口服液中绿原酸含量的影响因素研究[J].河北科技大学学报,2015,36(5):499-503.
[7]李纳,李姣娇,范蕾,等.用山银花与金银花制备双黄连口服液质量比较研究[J].西北药学杂志,2018,33(1):6-10.
[8]杨倩茹,赵媛媛,郝江波,等.金银花与山银花化学成分及其差异的研究进展[J].中国中药杂志,2016,41(7):1204-1211.
[9]笔雪艳,张清波,刘晓凤,等.7种常用中药制剂中金银花与山银花的应用初探[J].药物分析杂志,2010,30(11):2208-2211.
[10]刘静.肺炎糖浆的质量标准研究[J].西北药学杂志,2016,31(4):364-367.
[11]吴飞燕,冯宋岗,曾建国.金银花和山银花的鉴别与归属研究[J].中草药,2014,45(8):1150-1156.
[12]李红霞,王雪芹,李振国,等.不同产地金银花与山银花主要成分的含量比较[J].中国药房,2011,22(31):2935-2937.
[13]李泮霖,李楚源,刘孟华,等.基于UFLC-Triple-Q-TOFMS/MS技术的金银花、山银花化学成分比较[J].中南药学,2016,14(4):363-369.
[14]卢凤来,王磊,颜小捷,等.金银花和山银花的1 H-NMR指纹图谱鉴别[J].中成药,2017,39(1):222-224.
[15]刘亚楠,石典花,雷留成.金银花与山银花的HPLC指纹图谱鉴别研究[J].中草药,2017,48(4):773-776.
[16]杨倩茹,赵媛媛,郝江波,等.金银花与山银花化学成分及其差异的研究进展[J].中国中药杂志,2016,41(7):1204-1211.
[17]欧水平,张文志,陈灵.金银花与山银花抗病毒酚酸类和黄酮类成分的差异性研究[J].中国药房,2015,26(33):4750-4752.
[18]刘青芝,林永强,林晓,等.清热解毒口服液中山银花的检查方法研究[J].药学研究,2014,33(10):574-577.
[19]孙玲,樊晓兰,郭绮,等.一测多评法同时测定山银花药材中5种皂苷类成分的含量[J].中国药房,2017,28(18):2546-2549.
[20]王浩兵,邓力,马元春,等.基于UHPLC的金银花和山银花的鉴别及测定研究[J].中草药,2017,48(12):2516-2521.