阳离子树脂种类及用量对桑叶中1-脱氧野尻霉素纯化效果
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摘要
目的:确定桑叶中1-脱氧野尻霉素(DNJ)纯化的最佳树脂种类和用量。方法:采用LSI-010型(H型)、001×7(H型)、001×7(Na型)3种阳离子交换树脂纯化桑叶DNJ,并考察等量阳离子交换树脂对不同量桑叶DNJ纯化效果。结果:3种阳离子树脂对桑叶DNJ纯化效果差异较大,等量阳离子树脂对不同上样量的桑叶DNJ纯化效果不同。结论:001×7(Na型)阳离子交换树脂对桑叶DNJ纯化效果最好,阳离子树脂用量与药材量最佳比例为1∶1。
Objective:To select the optimal cation resin type and quantity for purification of 1-deoxymilithromycin(DNJ)in Mori Folium extract.Methods:Three cation resins of LSI-010(H),001×7(H),001×7(Na)were used to purify DNJ.The type and quantity of the resins were selected.Result:The purification capacity of three cation resins on DNJ was quite different.The adsorption effect of different cation resin quantity on DNJ was also different.Conclusion:The best resin for purification of DNJ is 001×7(Na).The optimum ratio of the amount of resin to the amount of the medicinal materials is 1∶1.
Objective:To select the optimal cation resin type and quantity for purification of 1-deoxymilithromycin(DNJ)in Mori Folium extract.Methods:Three cation resins of LSI-010(H),001×7(H),001×7(Na)were used to purify DNJ.The type and quantity of the resins were selected.Result:The purification capacity of three cation resins on DNJ was quite different.The adsorption effect of different cation resin quantity on DNJ was also different.Conclusion:The best resin for purification of DNJ is 001×7(Na).The optimum ratio of the amount of resin to the amount of the medicinal materials is 1∶1.
引文
[1] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:297.
[2] 任玉珍,王龙虎,蒋焕,等.不同采收期桑叶药材的质量比较[J].中国现代中药,2006,8(5):8-9.
[3] LI Y G,JI D F,ZHONG S,et al.Hybrid of 1-deoxynojirimycin and polysaccharide from mulberry leaves treat diabetes mellitus by activating PDX-1/insulin-1 signaling pathway and regulating the expression of glucokinase,phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in alloxan-induced diabetic mice [J].J Ethnopharmacol,2011,134(3):961-970.
[4] 周晓玲,孙凌云,张进,等.1-脱氧野尻霉素的来源及合成研究进展[J].蚕业科学,2011,37(1):105-110.
[5] 刘韦鋆,赵骏,高秀梅,等.1-脱氧野尻霉素的提取精制工艺[J].中华中医药学刊,2009,27(10):2079-2081.
[6] 刘超,徐立,史正琴,等.植物源1-脱氧野尻霉素分离纯化技术综述[J].蚕业科学,2014,40(3):544-550.
[7] 周玉飞,褚以文,王欣荣.HPLC-ELSD分析米格列醇和杂质1-脱氧野尻霉素[J].中国新药杂志,2012,21(4):375-377.
[8] 吴方睿.桑叶中1-脱氧野尻霉素提取分离工艺研究[D].合肥:合肥工业大学,2009.
[2] 任玉珍,王龙虎,蒋焕,等.不同采收期桑叶药材的质量比较[J].中国现代中药,2006,8(5):8-9.
[3] LI Y G,JI D F,ZHONG S,et al.Hybrid of 1-deoxynojirimycin and polysaccharide from mulberry leaves treat diabetes mellitus by activating PDX-1/insulin-1 signaling pathway and regulating the expression of glucokinase,phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in alloxan-induced diabetic mice [J].J Ethnopharmacol,2011,134(3):961-970.
[4] 周晓玲,孙凌云,张进,等.1-脱氧野尻霉素的来源及合成研究进展[J].蚕业科学,2011,37(1):105-110.
[5] 刘韦鋆,赵骏,高秀梅,等.1-脱氧野尻霉素的提取精制工艺[J].中华中医药学刊,2009,27(10):2079-2081.
[6] 刘超,徐立,史正琴,等.植物源1-脱氧野尻霉素分离纯化技术综述[J].蚕业科学,2014,40(3):544-550.
[7] 周玉飞,褚以文,王欣荣.HPLC-ELSD分析米格列醇和杂质1-脱氧野尻霉素[J].中国新药杂志,2012,21(4):375-377.
[8] 吴方睿.桑叶中1-脱氧野尻霉素提取分离工艺研究[D].合肥:合肥工业大学,2009.