运动营养食品中可卡因的检测
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摘要
食品中的可卡因不易被察觉,为分析运动营养食品的安全性。本文采用液-液萃取-气相色谱法检测尿液和血液中的可卡因,将待检测的人体尿液和血液生物样本进行预处理,优化液-液萃取条件后,采用GC-FID方法获取检测尿液和血液在不同条件下的色谱图。实验结果表明,色谱图显示检测尿液中的COC和检测血液中的COC、EME与基体干扰的分离效果明显;随着加入可卡因标准溶液质量浓度的提升,检测尿液和血液中的可卡因平均回收率越高,最高平均回收率分别是96.73%和98.43%,且平均最小相对标准偏差是1.39%,人体食用运动营养食品后日内和日间血液中EME和COD的平均相对标准偏差均低于2.3%,说明该方法可准确检测出食用运动营养食品后人体尿液和血液中的可卡因,从而判定运动营养食品的安全性。
Cocaine in food is hard to detect. In order to evaluate the safety of sports nutritional food, the method of liquid-liquid extraction-gas chromatography was used to detect cocaine in urine and blood. The human urine and blood biological samples to be detected were pretreated. After optimizing the conditions of liquid-liquid extraction, the chromatograms of urine and blood under different conditions were obtained by GC-FID. The results showed that the separation effect of COC in urine and COC, EME and matrix interference in blood were obvious. With the increase of the concentration of standard solution of cocaine, the average recovery rate of cocaine in urine and blood increased.The highest average recovery rate was 96.73% and 98.43%, respectively. The average minimum relative standard deviation was 1.39%. The average relative standard deviation of EME and COD in the blood of daytime and daytime after eating sports nutritional food was less than 2.3%.It shows that this method could accurately detect the cocaine in human urine and blood after eating sports nutritional food. It could evaluate the safety of sports nutritional food.
Cocaine in food is hard to detect. In order to evaluate the safety of sports nutritional food, the method of liquid-liquid extraction-gas chromatography was used to detect cocaine in urine and blood. The human urine and blood biological samples to be detected were pretreated. After optimizing the conditions of liquid-liquid extraction, the chromatograms of urine and blood under different conditions were obtained by GC-FID. The results showed that the separation effect of COC in urine and COC, EME and matrix interference in blood were obvious. With the increase of the concentration of standard solution of cocaine, the average recovery rate of cocaine in urine and blood increased.The highest average recovery rate was 96.73% and 98.43%, respectively. The average minimum relative standard deviation was 1.39%. The average relative standard deviation of EME and COD in the blood of daytime and daytime after eating sports nutritional food was less than 2.3%.It shows that this method could accurately detect the cocaine in human urine and blood after eating sports nutritional food. It could evaluate the safety of sports nutritional food.
引文
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[3]王路娜,陈一冰,张均,等.可卡因-苯丙胺调节转录肽对缺血-再灌注模型小鼠皮质突触可塑性的影响[J].中国脑血管病杂志,2017,14(3):127-132WANG Lu-na,CHEN Yi-bing,ZHANG Jun,et al.Effect of cocaine and amphetamine-regulated transcript peptides on cortical synaptic plasticity in the model mice of ischemia-reperfusion injury[J].Chinese Journal of Cerebrovascular Diseases,2017,14(3):127-132
[4]颜晓丽.超高效液相色谱-串联质谱法测定食品中4种罂粟壳生物碱[J].分析测试技术与仪器,2017,23(1):11-17YAN Xiao-li.Simultaneous determination of four opium poppy husk alkaloids in residues of foods using ultra performance liquid chromatography-tandem mass spectrometry[J].Analysis and Testing Technology and Instruments,2017,23(1):11-17
[5]王欢,胡磊,周明昊.固相萃取与液相色谱质谱联用技术测定化妆品中7种局部麻醉药[J].日用化学品科学,2017,40(9):30-34WANG Huan,HU Lei,ZHOU Ming-hao.Determination of seven local anesthetics in cosmetics by solid-phase extraction and liquid chromatography-mass spectrometry[J].Detergent&Cosmetics,2017,40(9):30-34
[6]薛庆利,薛山.体育运动食品功效作用机制及市场动态分析[J].闽南师范大学学报:自然科学版,2016,1:97-102XUE Qing-li,XUE Shan.Analysis on the action mechanism of nutrition function and the market dynamic status of sports food[J].Journal of Zhangzhou Teachers College(Natural Science Edition),2016,1:97-102
[7]范存霞,蔡毅,杨婷婷,等.光遗传技术在可卡因成瘾机制中的应用[J].中华行为医学与脑科学杂志,2015,24(6):573-576FAN Cun-xia,CAI Yi,YANG Ting-ting,et al.Advance in optogenetic application of cocaine addiction mechanisms[J].Chinese Journal of Behavioral Medicine and Brain Science,2015,24(6):573-576
[8]李安,张秀彤,王冬,等.固相萃取-超高效液相色谱-串联质谱法测定农田回用再生水中的9种雌激素[J].分析试验室,2016,1:73-77LI An,ZHANG Xiu-tong,WANG Dong,et al.Determination of 9 estrogens in reclaimed Water from farmland by Solid-Phase Extraction-Ultra-High performance liquid Chromatography-tandem Mass Spectrometry[J].Chinese Journal of Analysis Laboratory,2016,1:73-77
[9]陶弥锋.运动营养品中磺胺类成分的残留检测[J].食品研究与开发,2017,38(13):165-168TAO Mi-feng.Determination of sulfanilamide residue in sports nutrition[J].Food Research and Development,2017,38(13):165-168
[10]金永久,金艳红,金莉,等.阴离子交换固相萃取高效液相色谱测定尿中tt MA和SPMA[J].公共卫生与预防医学,2017,28(1):127-129JIN Yong-jiu,JIN Yan-hong,JIN Li,et al.Determination of tt MA and SPMA in urine by high performance liquid chromatography with anion exchange solid phase extraction[J].Journal of Public Health and Preventive Medicine,2017,28(1):127-129
[11]李洋,葛顺楠,李楠,等.药物成瘾记忆再巩固过程中脑内Zif268的激活[J].中华神经医学杂志,2015,14(12):1256-1265LI Yang,GE Shun-nan,LI Nan,et al.Activation of brain Zif268 in process of drug addictive memory reconsolidation[J].Chinese Journal of Neconsolidation,2015,14(12):1256-1265
[12]艾华,常翠青.运动营养食品中营养成分和功能因子研究进展[J].食品科学技术学报,2017,35(3):16-24AI Hua,CHANG Cui-qing.Research progress on nutritional components and functional factors in sports nutrition food[J].Journal of Food Science and Technology,2017,35(3):16-24
[13]Janda K D.Flagellin as carrier and adjuvant in cocaine vaccine development[J].Molecular Pharmaceutics,2015,12(2):653-662