GP-MSE/GC-MS分析夏枯草不同部位挥发性成分
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摘要
采用气流吹扫微萃取法(GP-MSE)提取夏枯草中挥发性成分,以GC-MS进行成分分离与鉴定,以峰面积归一化法进行定量,运用化学和PCA法比较其根、枝、叶、花中挥发性成分的种类和含量差异。结果表明:共分离出145种挥发性成分,根、枝、叶、花分别定性98种、98种、98种、124种成分,分别占各部位挥发性成分总量的66.53%、91.24%、89.32%和84.87%。花中主要含有烷烃类(29.01%)和含N、O-类物质(12.17%)。根和枝中主要贡献成分均为醛类(20.66%、21.18%)、酚类(13.23%、19.80%),叶中主要贡献成分为醇类(26.79%)和醛(15.15%)。结果显示花中成分与其他部位存在较大差异,而其他各部位间差异不大。本研究为夏枯草在食品、药品中进一步开发利用提供参考依据。
The volatile components in Prunella vulgaris were extracted by gas purge micro-extraction technique,and then analyzed by gas chromatography-mass spectrometry,the relative contents of individual constituents in the volatile components were determined by peak area normalizing method,the volatile components of root,branch,leaf and flower for Prunella vulgaris were compared by chemical method and principal component analysis. The results showed 145 peaks were isolated in Prunella vulgaris,98,98,98 and 124 components were identified in root,branch,leaf and flower,which accounted for 66.53%,91.24%,89.32% and 84.87% of the total peaks in each part. Flower mainly contains alkanes(29.01%)and N,O-compounds(12.17%). Aldehydes(20.66%、21.18%)and phenols(13.23%、19.8%%)were the main contributing components in root and branch. Alcohols(26.79%)and aldehydes(15.15%)were the main contributing components in leaf. The results showed that the chemical components were a great difference between the flower and the other parts. This study provides reference basis for development and utilization of Prunella vulgaris in food and medicine.
The volatile components in Prunella vulgaris were extracted by gas purge micro-extraction technique,and then analyzed by gas chromatography-mass spectrometry,the relative contents of individual constituents in the volatile components were determined by peak area normalizing method,the volatile components of root,branch,leaf and flower for Prunella vulgaris were compared by chemical method and principal component analysis. The results showed 145 peaks were isolated in Prunella vulgaris,98,98,98 and 124 components were identified in root,branch,leaf and flower,which accounted for 66.53%,91.24%,89.32% and 84.87% of the total peaks in each part. Flower mainly contains alkanes(29.01%)and N,O-compounds(12.17%). Aldehydes(20.66%、21.18%)and phenols(13.23%、19.8%%)were the main contributing components in root and branch. Alcohols(26.79%)and aldehydes(15.15%)were the main contributing components in leaf. The results showed that the chemical components were a great difference between the flower and the other parts. This study provides reference basis for development and utilization of Prunella vulgaris in food and medicine.
引文
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[2]陈宇航.夏枯草规范化种植技术及其药材质量控制[D].南京:南京农业大学,2011:3-4.
[3]陈蕾,周倩.夏枯草现代研究进展述要[J].海峡药学,2015,27(12):9-12.
[4]陈宇航,郭巧生,王澄亚.夏枯草本草及其入药部位变化的考证[J].中国中药杂志,2010,35(2):242-246.
[5]国家药典委员会。中华人民共和国药典,一部[M].北京:化学工业出版社,2005:197。
[6]关于批准DHA藻油、棉籽低聚糖等7种物品为新资源食品及其他相关规定的公告(2010年第3号).
[7]顾晓洁,李友宾,李萍,等.夏枯草花穗化学成分研究[J].中国中药杂志,2007,32(10):923-926.
[8]邓子煜,徐先祥,张小鸿,等.夏枯草药理学研究进展[J].安徽医学,2012,33(7):937-939。
[9]祝敏,展俊岭,赵二劳.夏枯草黄酮提取纯化工艺研究进展[J].化工时刊,2018,32(7):31-33.
[10]张金华,邱俊娜,王路,等.夏枯草化学成分及药理作用研究进展[J].中草药,2018,49(14):3432-3440.
[11]杨超.夏枯草挥发油抗子宫肌瘤活性及其作用机制研究[D].湖南:南华大学,2018:12-13.
[12]王海波,张芝玉,苏中武.国产3种夏枯草挥发油的成分[J].中国药学杂志.1994,29(11):652-653.
[13]Yang C,Piao X,Qiu J,et al.Gas purge microsyringe extraction for quantitative direct gas chromatographic-mass spectrometric analysis of volatile and semivolatile chemicals[J].J Chromatogra A,2011,1218(12):1549-1555.
[14]He M,Yang C,Geng R,et al.Monitoring of phthalates in foodstuffs using gas purge microsyringe extraction coupled with GC-MS[J].Anal Chim Acta,2015,879:63-68.
[15]Nan J,Wang J,Piao X,et al.Novel and rapid method for determination of organophosphorus pesticide residues in edible fungus using direct gas purge microsyringe extraction coupled on-line with gas chromatography-mass spectrometry[J].Talanta,2015,142:64-71.