~(60)Co-γ射线辐照金柑花粉对果实生长发育及内源激素含量的影响
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摘要
为减少遂川金柑种子数,提高其品质和市场竞争力,本研究利用1 000 Gy的60Co-γ射线对遂川金柑花粉进行辐照处理,之后授粉于遂川金柑,并以未经辐照处理为对照(CK),研究辐照处理对遂川金柑果实生长发育及内源激素含量变化的影响。结果表明,与CK相比,辐照处理后遂川金柑饱满种子数减少,且果实可滴定酸含量、总糖含量、Vc含量和可溶性固形物(TSS)含量与CK差异不显著,但果实的大小和单果重明显降低。授粉后30 d,辐照处理的果实内源生长素(IAA)、赤霉素(GA)、细胞分裂素(ZT)、(GA+IAA+ZT)/ABA明显低于CK。本研究结果为60Co-γ射线辐照育种在金柑上的应用提供了一定的理论参考依据。
In order to reduce the number of seeds of Fortunella crassifolia CV. Suichuangjingan,improve it's quality and competitiveness of the market,the pollen of Fortunella crassifolia CV. Suichuangjingan was treated with 1 000 Gy ~(60)Co-γ ray,the pollinated it to Fortunella crassifolia CV. Suichuangjingan,and the non-irradiated pollen was used as control. The effects of radiation pollen on fruit growth,development and endogenous hormones contents were studied.The results showed that compared with the no irradiated pollen treatment,radiating pollen decreased the number of seeds of Fortunella crassifolia CV. Suichuangjingan,and the titratable acid content,total sugar content,Vc content and TSS content had no significant difference,but the fruit size and fruit weight were decreased obviously. 30 d after the pollination,the endogenous of IAA,GA,ZT contents and( GA + IAA + ZT)/ABA ratio of pollen irradiation group was apparently lower than CK. The results gives a reference for the application of ~(60)Co-γ ray radiation breeding in Kumquat.
In order to reduce the number of seeds of Fortunella crassifolia CV. Suichuangjingan,improve it's quality and competitiveness of the market,the pollen of Fortunella crassifolia CV. Suichuangjingan was treated with 1 000 Gy ~(60)Co-γ ray,the pollinated it to Fortunella crassifolia CV. Suichuangjingan,and the non-irradiated pollen was used as control. The effects of radiation pollen on fruit growth,development and endogenous hormones contents were studied.The results showed that compared with the no irradiated pollen treatment,radiating pollen decreased the number of seeds of Fortunella crassifolia CV. Suichuangjingan,and the titratable acid content,total sugar content,Vc content and TSS content had no significant difference,but the fruit size and fruit weight were decreased obviously. 30 d after the pollination,the endogenous of IAA,GA,ZT contents and( GA + IAA + ZT)/ABA ratio of pollen irradiation group was apparently lower than CK. The results gives a reference for the application of ~(60)Co-γ ray radiation breeding in Kumquat.
引文
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[14]Kundu M,Dubey A,Srivastav M,Malik S.Induction of haploid plants in citrus through gamma-irradiated pollen and ascertainment of ovule age for maximum recovery of haploid plantlets[J].Turkish Journal of Biology,2017,41(3):469-483
[15]Aleza P,Juárez J,Hernández M,Pina J,Ollitrault P,Navarro L.Recovery and characterization of a Citrus clementina Hort.ex Tan.‘Clemenules’haploid plant selected to establish the reference whole Citrus genome sequence[J].BMC Plant Biology,2009,9(1):110-126
[16]Yahata M,Yasuda K,Nagasawa K,Harusaki S,Komatsu H,Kunitake H.Production of haploid plant of‘Banpeiyu’pummelo[Citrus maxima(Burm.)Merr.]by pollination with soft X-rayirradiated pollen[J].Journal of the Japanese Society for Horticultural Science,2010,79(3):239-245
[17]Wang S M,Lan H,Jia H H,Xie K D,Wu X M,Chen C L,Guo WW.Induction of parthenogenetic haploid plants using gamma irradiated pollens in‘Hirado Buntan’pummelo(Citrus grandis[L.]Osbeck)[J].Scientia Horticulturae,2016,207(5):233-239
[18]周鹤侍,薛振洪,沈光献.辐照柑桔花粉诱发少核效应的研究[J].浙江柑桔,1984(3):9-11
[19]Ogata T,Takeichi T,Matsunaga K,Hasegawa K,Yamane S,Sugiyama K.Seed abortion of‘Tosa-Buntan’pummelo pollinated with soft-X-irradiated pollens[J].Scientia Horticulturae,2008,116(2):180-185
[20]陈学森,郭文武,徐娟,丛佩华,王力荣,刘崇怀,李秀根,吴树敬,姚玉新,陈晓流.主要果树果实品质遗传改良与提升实践[J].中国农业科学,2015,48(17):3524-3540
[21]Ram S.Naturally occurring hormones of mango and their role in growth and drop of the fruit[J].Acta Horticulturae,1992,321(321):400-411
[22]Kiyohide K,Yamamoto Y,Goto G.Changes in ABA,IAA and GAs contents in reproductive organs of satsuma mandarin Kiyohide kojima1[J].Journal of the Japanese Society for Horticultural Science,1996,65(2):237-243
[23]张涓涓,杨莉,刘德春,刘山蓓,徐炳星,周施清,毛卫平,刘勇.马家柚果实品质与土壤、叶片、果实矿质养分的相关性分析[J].江西农业大学学报,2015,37(5):811-818
[24]Yang J,Zhang J,Wang Z,Zhu Q,Wang W.Hormonal changes in the grains of rice subjected to water stress during grain filling[J].Plant Physiology,2001,127(1):315-323
[25]于海芹,印洁,黄昌军,李永平,舒庆尧,刘勇.烟草种子和花粉的γ射线适宜诱变剂量探究[J].分子植物育种,2016,14(10):2749-2755
[26]杜保伟,尚霄丽,李成斌,李靖.60Co-γ射线对杏李花粉的辐射效应[J].果树学报,2010,27(4):533-536
[27]林俊,张克中,贾月慧,张启翔,马莉.辐照花粉辅助授粉克服百合受精前障碍的研究[J].核农学报,2008,22(6):776-780
[28]钱林庆,周云龙,孙凡捷,任陈晨.质子束辐射金花葵种子和花粉的生物学效应[J].中国农业科技导报,2015,17(6):37-43
[29]邱燕萍,向旭,王碧青,张居薇,袁沛元.荔枝三种结实类型内源激素的平衡与坐果的机理[J].果树学报,1998,15(1):39-43
[30]贾彦丽,温陟良.无核果实研究进展[J].河北农业大学学报,2003,26(z1):68-71
[31]任莹,张春芬,小森贞男,邓舒,肖蓉,罗慧珍,孟玉平,曹秋芬.γ射线辐射花粉对苹果受精及胚发育的影响[J].分子植物育种,2014,12(5):937-942
[32]石庆华,刘平,刘孟军.果树倍性育种研究进展[J].园艺学报,2012,39(9):1639-1654
[33]张斯淇,徐强,邓秀新.无融合生殖与柑橘多胚现象的研究进展[J].植物科学学报,2014,32(1):88-96
[34]黄华宁,贺军虎,赵小青,陈华蕊,陈业渊.胚胎败育与内源激素变化的关系研究进展[J].安徽农业科学,2013,41(32):12534-12536
[35]贺军虎,马锋旺,束怀瑞,陈业渊,赵小青,魏军亚,陈华蕊.‘金煌’杧果胚正常与胚败育果实内源激素的变化[J].园艺学报,2012,39(6):1167-1174
[36]陆军,张建和.玉环柚果实发育后期内源激素含量的变化[J].中国南方果树,1997,26(5):3-5
[37]Keita S,Masami M.Production of seedless watermelon usingsoft-X-irradiated pollen[J].Scientia Hortichlturae,2000,84(3):255-264
[38]沈德绪.果树育种学[M].第二版.北京:中国农业出版社,2000
[2]文蓉,石宠良,袁卫建.遂川金柑品质下降原因分析与改良措施[J].现代园艺,2014,23(12):37-38
[3]韦杰,黄郴,黄寿辉.柑橘新品种滑皮金柑高产优质中试栽培技术研究[J].农业研究与应用,2017(1):29-31
[4]张宏梓.金柑优株初选研究[J].林业科技开发,2005,19(6):45-47
[5]李靖,尚霄丽,张建鹏.辐射对果树花粉发芽率及远缘杂交亲和性的影响[J].核农学报,2006,20(5):395-397
[6]陆艳婷,陈金跃,张小明,叶胜海,王俊敏,金庆生.浙江省水稻辐射育种研究进展[J].核农学报,2017,31(8):1500-1508
[7]李杰,张银洁,匡萍,喻兰,郑春.快中子辐照对蝴蝶兰离体茎段生理特性的影响[J].核农学报,2017,31(6):1153-1158
[8]李风童,包建忠,孙叶,刘春贵,马辉,张甜,陈秀兰.60Co-γ射线辐照德国鸢尾杂交种子的生物效应[J].核农学报,2017,31(8):1469-1474
[9]彭振英,王兴军,田海莹,郑玲,单雷,范仲学,边斐,郭峰,王莹莹,万书波.花生60Co-γ辐射诱变和突变体库的构建[J].核农学报,2016,30(3):422-429
[10]邓秀新,郭文武,孙绪华.我国无核柑桔类型选育研究进展---文献综述[J].园艺学报,1996,23(3):235-241
[11]周育彬,彭定秀,陈善春,张菊英,戴增国.中育7号和中育8号无核甜橙的经济性状及遗传学鉴定[J].中国柑桔,1990,19(4):9-11,49-50
[12]李志强,唐小浪,吴绍彝,彭成绩,罗敏.柑橘新品种少核红橙的选育[J].中国果树,2008(5):5-7,81
[13]李植彝,岳仁芳.柑桔60Co-γ射线辐射育种研究初报[J].广西热作科技,1990(2):12-15
[14]Kundu M,Dubey A,Srivastav M,Malik S.Induction of haploid plants in citrus through gamma-irradiated pollen and ascertainment of ovule age for maximum recovery of haploid plantlets[J].Turkish Journal of Biology,2017,41(3):469-483
[15]Aleza P,Juárez J,Hernández M,Pina J,Ollitrault P,Navarro L.Recovery and characterization of a Citrus clementina Hort.ex Tan.‘Clemenules’haploid plant selected to establish the reference whole Citrus genome sequence[J].BMC Plant Biology,2009,9(1):110-126
[16]Yahata M,Yasuda K,Nagasawa K,Harusaki S,Komatsu H,Kunitake H.Production of haploid plant of‘Banpeiyu’pummelo[Citrus maxima(Burm.)Merr.]by pollination with soft X-rayirradiated pollen[J].Journal of the Japanese Society for Horticultural Science,2010,79(3):239-245
[17]Wang S M,Lan H,Jia H H,Xie K D,Wu X M,Chen C L,Guo WW.Induction of parthenogenetic haploid plants using gamma irradiated pollens in‘Hirado Buntan’pummelo(Citrus grandis[L.]Osbeck)[J].Scientia Horticulturae,2016,207(5):233-239
[18]周鹤侍,薛振洪,沈光献.辐照柑桔花粉诱发少核效应的研究[J].浙江柑桔,1984(3):9-11
[19]Ogata T,Takeichi T,Matsunaga K,Hasegawa K,Yamane S,Sugiyama K.Seed abortion of‘Tosa-Buntan’pummelo pollinated with soft-X-irradiated pollens[J].Scientia Horticulturae,2008,116(2):180-185
[20]陈学森,郭文武,徐娟,丛佩华,王力荣,刘崇怀,李秀根,吴树敬,姚玉新,陈晓流.主要果树果实品质遗传改良与提升实践[J].中国农业科学,2015,48(17):3524-3540
[21]Ram S.Naturally occurring hormones of mango and their role in growth and drop of the fruit[J].Acta Horticulturae,1992,321(321):400-411
[22]Kiyohide K,Yamamoto Y,Goto G.Changes in ABA,IAA and GAs contents in reproductive organs of satsuma mandarin Kiyohide kojima1[J].Journal of the Japanese Society for Horticultural Science,1996,65(2):237-243
[23]张涓涓,杨莉,刘德春,刘山蓓,徐炳星,周施清,毛卫平,刘勇.马家柚果实品质与土壤、叶片、果实矿质养分的相关性分析[J].江西农业大学学报,2015,37(5):811-818
[24]Yang J,Zhang J,Wang Z,Zhu Q,Wang W.Hormonal changes in the grains of rice subjected to water stress during grain filling[J].Plant Physiology,2001,127(1):315-323
[25]于海芹,印洁,黄昌军,李永平,舒庆尧,刘勇.烟草种子和花粉的γ射线适宜诱变剂量探究[J].分子植物育种,2016,14(10):2749-2755
[26]杜保伟,尚霄丽,李成斌,李靖.60Co-γ射线对杏李花粉的辐射效应[J].果树学报,2010,27(4):533-536
[27]林俊,张克中,贾月慧,张启翔,马莉.辐照花粉辅助授粉克服百合受精前障碍的研究[J].核农学报,2008,22(6):776-780
[28]钱林庆,周云龙,孙凡捷,任陈晨.质子束辐射金花葵种子和花粉的生物学效应[J].中国农业科技导报,2015,17(6):37-43
[29]邱燕萍,向旭,王碧青,张居薇,袁沛元.荔枝三种结实类型内源激素的平衡与坐果的机理[J].果树学报,1998,15(1):39-43
[30]贾彦丽,温陟良.无核果实研究进展[J].河北农业大学学报,2003,26(z1):68-71
[31]任莹,张春芬,小森贞男,邓舒,肖蓉,罗慧珍,孟玉平,曹秋芬.γ射线辐射花粉对苹果受精及胚发育的影响[J].分子植物育种,2014,12(5):937-942
[32]石庆华,刘平,刘孟军.果树倍性育种研究进展[J].园艺学报,2012,39(9):1639-1654
[33]张斯淇,徐强,邓秀新.无融合生殖与柑橘多胚现象的研究进展[J].植物科学学报,2014,32(1):88-96
[34]黄华宁,贺军虎,赵小青,陈华蕊,陈业渊.胚胎败育与内源激素变化的关系研究进展[J].安徽农业科学,2013,41(32):12534-12536
[35]贺军虎,马锋旺,束怀瑞,陈业渊,赵小青,魏军亚,陈华蕊.‘金煌’杧果胚正常与胚败育果实内源激素的变化[J].园艺学报,2012,39(6):1167-1174
[36]陆军,张建和.玉环柚果实发育后期内源激素含量的变化[J].中国南方果树,1997,26(5):3-5
[37]Keita S,Masami M.Production of seedless watermelon usingsoft-X-irradiated pollen[J].Scientia Hortichlturae,2000,84(3):255-264
[38]沈德绪.果树育种学[M].第二版.北京:中国农业出版社,2000