中国深圳和芬兰艾斯堡地区健康婴幼儿肠道菌群的比较
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摘要
目的探索中国与欧洲沿海发达地区婴幼儿肠道菌群的结构差异及随年龄增长的变化趋势。方法招募深圳地区健康婴幼儿35名并收集其粪便样本,采用Illumina的MiSeq平台对肠道菌群的16S rRNA V4序列进行测序。同时,结合40名芬兰艾斯堡地区健康婴幼儿肠道菌群数据,进行两地儿童菌群结构比较。结果中国与芬兰地区幼儿肠道菌群结构较为一致,在物种多样性及均匀度方面差异无统计学意义。菌属水平分析中,两组幼儿肠道内均呈现以拟杆菌属、双歧杆菌属为主导的菌群结构。回归分析表明,幼儿肠道内菌群多样性随年龄的增长明显增加(R~2=0.174,P<0.05),0～1岁与1～3岁幼儿的菌群结构差异有统计学意义(R~2=0.079,P<0.05)。韦荣球菌、肠球菌和布劳特菌等菌属随年龄增长丰度明显降低,而粪杆菌、瘤胃球菌、罗斯菌等菌属显著增加。结论生命早期肠道菌群的发育主要与年龄因素密切相关,地区因素为次要因素。
Objective To explore the structural differences in gut microbiota between infants from Chinese and European developed coastal cities, and the changing trend of microbial community along with age. Methods We enrolled a total of 35 healthy infants at Shenzhen and sampled fecal specimens. Bacterial 16S rRNA V4 region was sequenced using Illumina MiSeq platform. We also collected the fecal microbiota data of 40 healthy infants in Finland to conduct comparison analysis. Results The intestinal microbiota of infants from China and Finland were relatively consistent, and the differences in bacterial diversity and evenness were not statistically significant. At the genus level, Bacteroides and Bifidobacteria were the dominated genera. Regression analysis revealed that the diversity of intestinal microbiota increased significantly along with age(R~2=0.174,P<0.05), and the differences in intestinal microbiota between infants aged 0-1 year and 1-3 years were also significant(R~2=0.079,P<0.05). The abundances of Veillonella, Enterococcus and Brauterella decreased during development, whereas those of Faecalibacterium, Ruminococcus and Rothia increased gradually. Conclusion Age is mainly associated with the development of intestinal microflora in early life, and region acts as the secondary influence factor.
Objective To explore the structural differences in gut microbiota between infants from Chinese and European developed coastal cities, and the changing trend of microbial community along with age. Methods We enrolled a total of 35 healthy infants at Shenzhen and sampled fecal specimens. Bacterial 16S rRNA V4 region was sequenced using Illumina MiSeq platform. We also collected the fecal microbiota data of 40 healthy infants in Finland to conduct comparison analysis. Results The intestinal microbiota of infants from China and Finland were relatively consistent, and the differences in bacterial diversity and evenness were not statistically significant. At the genus level, Bacteroides and Bifidobacteria were the dominated genera. Regression analysis revealed that the diversity of intestinal microbiota increased significantly along with age(R~2=0.174,P<0.05), and the differences in intestinal microbiota between infants aged 0-1 year and 1-3 years were also significant(R~2=0.079,P<0.05). The abundances of Veillonella, Enterococcus and Brauterella decreased during development, whereas those of Faecalibacterium, Ruminococcus and Rothia increased gradually. Conclusion Age is mainly associated with the development of intestinal microflora in early life, and region acts as the secondary influence factor.
引文
[1] 郎田田,雷姝敏,陈红.肠道菌群与饮食、疾病关系的研究进展[J].食品安全导刊,2018(3):34.
[2] WU Shuli,ZHU Hua.Effect of delivery modes on the intestinal flora in 3 days infants[J].J Pediatr Pharmacy,2017,23(12):4-6.(in Chinese) 武书丽,朱华.分娩方式对新生儿出生后3天内肠道菌群的影响[J].儿科药学杂志,2017,23(12):4-6.
[3] LU Wei,ZHANG Hongbo,HUANG Juan,et al.Influence of feeding model on stool characteristics and intestinal flora in infants delivered by cesarean[J].J Clin Pediatr,2011,29(5):450-453.(in Chinese) 陆薇,张红波,黄娟,等.喂养方式对剖宫产婴儿大便性状及肠道菌群的影响[J].临床儿科杂志,2011,29(5):450-453.
[4] ZHANG Shuiping,LIU Liming,SUN Xiaomian,et al.A research on relationship between intestinal microflora and supplementary food adding[J].Chin J Woman Children Health Res,2007,18(6):461-466.(in Chinese) 张水平,刘黎明,孙晓勉,等.婴幼儿肠道菌群与辅食添加状况的相关性研究[J].中国妇幼健康研究,2007,18(6):461-466.
[5] Rutayisire E,Huang K,Liu Y,et al.The mode of delivery affects the diversity and colonization pattern of the gut microbiota during the first year of infants′ life:a systematic review[J].BMC Gastroenterol,2016,16(1):86.
[6] Vatanen T,Kostic A,D Hennezel E,et al.Variation in microbiome LPS immunogenicity contributes to autoimmunity in humans[J].Cell,2016,165(4):842-853.
[7] HUANG Wenxian,WANG Heping,WANG Yulei,et al.High-throughput sequencing reveals the change of gut microbiota in infants with pneumonia following antibiotic treatment[J].Chin J Microbiol,2016,28(5):497-500.(in Chinese) 黄文献,王和平,王玉蕾,等.高通量测序检测肺炎婴幼儿抗生素治疗前后肠道菌群变化[J].中国微生态学杂志,2016,28(5):497-500.
[8] Min L,Mei W,Donovan SM.Early development of the gut microbiome and immune-mediated childhood disorders[J].Seminars Reproduct Med,2014,32(1):74-86.
[9] Marie-Claire A,Stiemsma LT,Nelly A,et al.The intestinal microbiome in early life:health and disease[J].Front Immunol,2014,5:427.
[10] Neu J,Mai V.The developing intestinal microbiome and its relationship to health and disease[J].J Perinatol Offic J California Perinatal Assoc,2011,31(1):S29.
[11] Christophe C,Eve D,Lawson PA,et al.Bacteroides xylanisolvens sp.nov.,a xylan-degrading bacterium isolated from human faeces[J].Int J Syst & Evolut Microbiol,2008,58(Pt 4):1008.
[12] Matsuki T,Pédron T,Regnault B,et al.Epithelial cell proliferation arrest induced by lactate and acetate from Lactobacillus casei and Bifidobacterium breve[J].PLoS One,2013,8(4):e63053.
[13] Talarico ST,Santos FE,Brandt KG,et al.Anaerobic bacteria in the intestinal microbiota of Brazilian children[J].Clinics,2017,72(3):154-160.
[14] Hopkins MJ,Macfarlane GT,Furrie E,et al.Characterisation of intestinal bacteria in infant stools using real-time PCR and northern hybridisation analyses[J].Fems Microbiol Ecol,2005,54(1):77-85.
[15] Fan W,Huo G,Li X,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in infants during the six months of life[J].J Microbiol Biotechnol,2014,24(2):133-143.
[16] Tanya Y,Rey FE,Manary MJ,et al.Human gut microbiome viewed across age and geography[J].Nature,2012,486(7402):222-227.
[17] Endo A,P?rtty A,Kalliom?ki M,et al.Long-term monitoring of the human intestinal microbiota from the 2nd week to 13 years of age[J].Anaerobe,2014,28(5):149-156.
[18] Anders BM,Thomas Hjort S,Martin Iain B,et al.Establishment of intestinal microbiota during early life:a longitudinal,explorative study of a large cohort of Danish infants[J].Appl Environ Microbiol,2014,80(9):2889-2900.
[2] WU Shuli,ZHU Hua.Effect of delivery modes on the intestinal flora in 3 days infants[J].J Pediatr Pharmacy,2017,23(12):4-6.(in Chinese) 武书丽,朱华.分娩方式对新生儿出生后3天内肠道菌群的影响[J].儿科药学杂志,2017,23(12):4-6.
[3] LU Wei,ZHANG Hongbo,HUANG Juan,et al.Influence of feeding model on stool characteristics and intestinal flora in infants delivered by cesarean[J].J Clin Pediatr,2011,29(5):450-453.(in Chinese) 陆薇,张红波,黄娟,等.喂养方式对剖宫产婴儿大便性状及肠道菌群的影响[J].临床儿科杂志,2011,29(5):450-453.
[4] ZHANG Shuiping,LIU Liming,SUN Xiaomian,et al.A research on relationship between intestinal microflora and supplementary food adding[J].Chin J Woman Children Health Res,2007,18(6):461-466.(in Chinese) 张水平,刘黎明,孙晓勉,等.婴幼儿肠道菌群与辅食添加状况的相关性研究[J].中国妇幼健康研究,2007,18(6):461-466.
[5] Rutayisire E,Huang K,Liu Y,et al.The mode of delivery affects the diversity and colonization pattern of the gut microbiota during the first year of infants′ life:a systematic review[J].BMC Gastroenterol,2016,16(1):86.
[6] Vatanen T,Kostic A,D Hennezel E,et al.Variation in microbiome LPS immunogenicity contributes to autoimmunity in humans[J].Cell,2016,165(4):842-853.
[7] HUANG Wenxian,WANG Heping,WANG Yulei,et al.High-throughput sequencing reveals the change of gut microbiota in infants with pneumonia following antibiotic treatment[J].Chin J Microbiol,2016,28(5):497-500.(in Chinese) 黄文献,王和平,王玉蕾,等.高通量测序检测肺炎婴幼儿抗生素治疗前后肠道菌群变化[J].中国微生态学杂志,2016,28(5):497-500.
[8] Min L,Mei W,Donovan SM.Early development of the gut microbiome and immune-mediated childhood disorders[J].Seminars Reproduct Med,2014,32(1):74-86.
[9] Marie-Claire A,Stiemsma LT,Nelly A,et al.The intestinal microbiome in early life:health and disease[J].Front Immunol,2014,5:427.
[10] Neu J,Mai V.The developing intestinal microbiome and its relationship to health and disease[J].J Perinatol Offic J California Perinatal Assoc,2011,31(1):S29.
[11] Christophe C,Eve D,Lawson PA,et al.Bacteroides xylanisolvens sp.nov.,a xylan-degrading bacterium isolated from human faeces[J].Int J Syst & Evolut Microbiol,2008,58(Pt 4):1008.
[12] Matsuki T,Pédron T,Regnault B,et al.Epithelial cell proliferation arrest induced by lactate and acetate from Lactobacillus casei and Bifidobacterium breve[J].PLoS One,2013,8(4):e63053.
[13] Talarico ST,Santos FE,Brandt KG,et al.Anaerobic bacteria in the intestinal microbiota of Brazilian children[J].Clinics,2017,72(3):154-160.
[14] Hopkins MJ,Macfarlane GT,Furrie E,et al.Characterisation of intestinal bacteria in infant stools using real-time PCR and northern hybridisation analyses[J].Fems Microbiol Ecol,2005,54(1):77-85.
[15] Fan W,Huo G,Li X,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in infants during the six months of life[J].J Microbiol Biotechnol,2014,24(2):133-143.
[16] Tanya Y,Rey FE,Manary MJ,et al.Human gut microbiome viewed across age and geography[J].Nature,2012,486(7402):222-227.
[17] Endo A,P?rtty A,Kalliom?ki M,et al.Long-term monitoring of the human intestinal microbiota from the 2nd week to 13 years of age[J].Anaerobe,2014,28(5):149-156.
[18] Anders BM,Thomas Hjort S,Martin Iain B,et al.Establishment of intestinal microbiota during early life:a longitudinal,explorative study of a large cohort of Danish infants[J].Appl Environ Microbiol,2014,80(9):2889-2900.