2,6-dansyl azide快速测定血清中H_2S含量
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摘要
对测定血清中H2S浓度的方法进行了改进。基于2,6-dansyl azide本身不发荧光,但与H2S作用后显示强烈荧光这一特性,一步法合成2,6-dansyl azide后采用PBS/DM SO体系(pH 7.5)替代PBS/Tween-20体系(pH 7.5)测定内源性H2S。数据分析显示改进的方法具有更低的荧光背景值。应用本方法成功对6只成年健康SD大鼠的血浆H2S水平进行了测定。
An improved method for measuring H2 S concentration in serum was described. The 2,6-dansyl azide(2,6-DNS-Az) itself does not fluoresce,however,it can emit a strong fluorescence when reacts with H2 S. Based on this characteristic,2,6-DNS-Az was synthesized by one-step method,then endogenous H2 S was determined with PBS buffer/DMSO(pH7. 5) instead of PBS buffer or PBS buffer/Tween-20(pH7. 5). The study indicated that the improved assay method has a better determination effect with lower fluorescence background value. Furthermore,this method was used successfully for measuring endogenous hydrogen sulfide concentration in plasma from 6 healthy adult SD rats.
An improved method for measuring H2 S concentration in serum was described. The 2,6-dansyl azide(2,6-DNS-Az) itself does not fluoresce,however,it can emit a strong fluorescence when reacts with H2 S. Based on this characteristic,2,6-DNS-Az was synthesized by one-step method,then endogenous H2 S was determined with PBS buffer/DMSO(pH7. 5) instead of PBS buffer or PBS buffer/Tween-20(pH7. 5). The study indicated that the improved assay method has a better determination effect with lower fluorescence background value. Furthermore,this method was used successfully for measuring endogenous hydrogen sulfide concentration in plasma from 6 healthy adult SD rats.
引文
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[3]Stipanuk M H,Beck P W.Biochem J,1982,206(2):267
[4]Hughes M N,Centelles M N,Moore K P.Free Radical Biol&Med,2009,47(10):1346
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[6]Radfordknoery J,Cutter G A.Anal Chem,1993,65(8):976
[7]Takadate A,Tahara K,Fujino H,Goya S.J Pharm Soc Japan,1986,106:36
[8]Kazemi F,Kiasat A R,Sayyahi S.Cheminform,2005,36(4):1813
[9]Peng H,Cheng Y,Dai C,King AL,Predmore BL,Lefer DJ,Wang B.Angew Chem Int Ed Engl,2011,50(41):9672
[10]Wang B,Burroughs S,Chen W,Peng H.Current Organic Chemistry,2013,17(6):641
[11]Wang K,Peng H,Ni N,Dai C,Wang B.J Fluoresc,2014,24(1):1