2型糖尿病大鼠六味地黄丸治疗后血脂、血糖以及IL-2和IL-10水平的变化
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摘要
目的探讨六味地黄丸治疗2型糖尿病大鼠的疗效。方法构建2型糖尿病、中医分类的阴虚热盛型和气阴两虚型三种的大鼠模型,模型组均采用六味地黄丸干预治疗,正常对照组大鼠采用安慰剂干预,检测四组大鼠干预前后的血糖(Glu)、三酰甘油(TG)、总胆固醇(TC)以及细胞因子IL-2和IL-10的水平。结果正常对照组的Glu、TC和TG均无明显变化。模型组干预治疗后的Glu和TG水平均明显下降(P<0.05),而TC除阴虚热盛组水平下降明显(P<0.05)外,其余各组的变化不明显;模型组IL-2和IL-10均呈明显弱表达,而治疗后两者的升高较为明显(P<0.01)。结论在大鼠模型中,六味地黄丸治疗2型糖尿病的疗效明显,可引起细胞因子IL-2和IL-10水平的高表达,其疗效可能与Th1/Th2平衡相关。
Objective To explore the curative effect of Liuwei Dihuang pill for the treatment of type 2 diabetes mellitus(T2DM) rats.Methods Three kinds of rat models were established,including T2DM model(T2DM group),Yin-Xu-Re-Sheng model(YXRS group) and Qi-Yin-Liang-Xu model(QYLX group).Rats of normal control group received placebo intervention,and rats of model groups received Liuwei Dihuang pill intervention.The levels of glucose(Glu),triglyceride(TG),total cholesterol(TC),IL-2 and IL-10 before and after intervention of four groups were detected.Results The levels of Glu,TC and TG of normal control group before and after intervention had no significant change(P >0.05).The levels of Glu and TG of model groups decreased significantly after intervention(P <0.05);After intervention,the TC level decreased significantly in YXRS group(P <0.05),and had no significant change in the other two groups(P >0.05).Before intervention,the levels of IL-2 and IL-10 of model groups showed obviously low expression,while after intervention,the IL-2 and IL-10 levels increased significantly(P <0.01).Conclusions Liuwei Dihuang pill has significant efficacy for T2DM rats,which can lead to high expressions of IL-2 and IL-10.The efficacy may relate to Th1/Th2 balance.
Objective To explore the curative effect of Liuwei Dihuang pill for the treatment of type 2 diabetes mellitus(T2DM) rats.Methods Three kinds of rat models were established,including T2DM model(T2DM group),Yin-Xu-Re-Sheng model(YXRS group) and Qi-Yin-Liang-Xu model(QYLX group).Rats of normal control group received placebo intervention,and rats of model groups received Liuwei Dihuang pill intervention.The levels of glucose(Glu),triglyceride(TG),total cholesterol(TC),IL-2 and IL-10 before and after intervention of four groups were detected.Results The levels of Glu,TC and TG of normal control group before and after intervention had no significant change(P >0.05).The levels of Glu and TG of model groups decreased significantly after intervention(P <0.05);After intervention,the TC level decreased significantly in YXRS group(P <0.05),and had no significant change in the other two groups(P >0.05).Before intervention,the levels of IL-2 and IL-10 of model groups showed obviously low expression,while after intervention,the IL-2 and IL-10 levels increased significantly(P <0.01).Conclusions Liuwei Dihuang pill has significant efficacy for T2DM rats,which can lead to high expressions of IL-2 and IL-10.The efficacy may relate to Th1/Th2 balance.
引文
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[7]郑镇雄,郑元裕,林春光,等.松叶消渴方治疗2型糖尿病的临床观察[J].中国医药指南,2014,12(32):44-45.
[8]Lee CL,Chiu PC,Lam KK,et al.Differential actions of glycodelin-A on Th-1 and Th-2 cells:a paracrine mechanism that could produce the Th-2 dominant environment during pregnancy[J].Hum Reprod,2011,26(3):517-526.
[9]秦婷.六味地黄丸治疗2型糖尿病的临床应用[J].浙江中医药大学学报,2008,32(4):563.
[2]姜华.脾阴虚与2型糖尿病相关性的临证探讨[J].天津中医药,2008,25(2):132.
[3]李敬林,王太一,王禄增,等.2型糖尿病证病结合动物模型的研究[J].中国比较医学杂志,2007,17(8):473-475.
[4]万莉,王曼晋,杨秋.实验性2型糖尿病兔模型的建立及其血清物质变化研究[J].现代检验医学杂志,2011,26(2):55-58.
[5]廖丽锦,黄霞,李颜屏.六味地黄丸辅助消渴丸治疗2型气阴两虚糖尿病探讨[J].糖尿病天地,2009,3(12):547-550.
[6]张庆祝.六味地黄丸治疗阴虚型2型糖尿病疗效观察[J].现代中西医结合杂志,2006,15(10):1282.
[7]郑镇雄,郑元裕,林春光,等.松叶消渴方治疗2型糖尿病的临床观察[J].中国医药指南,2014,12(32):44-45.
[8]Lee CL,Chiu PC,Lam KK,et al.Differential actions of glycodelin-A on Th-1 and Th-2 cells:a paracrine mechanism that could produce the Th-2 dominant environment during pregnancy[J].Hum Reprod,2011,26(3):517-526.
[9]秦婷.六味地黄丸治疗2型糖尿病的临床应用[J].浙江中医药大学学报,2008,32(4):563.