摘要
该研究基于课题组前期发现的"藻戟遂芫"与甘草配伍致毒增毒特点,以利尿作用和肠道菌群结构为表征指标,拓展性开展功效相似、基原相近、化学成分类型类同的中药巴豆霜与甘草合用可能导致的减效增毒作用研究。结果显示,甘草与高剂量巴豆霜合用,可减缓巴豆霜的快速利尿作用,表现为一定的降效作用趋势;巴豆霜高、低剂量均可显著损伤小鼠小肠组织,并使其肠道菌群组成结构发生显著变化。低剂量巴豆霜合用甘草后可使有害菌属Streptococcus(链球菌属)和Rikenellaceae_ukn的水平显著升高,高剂量巴豆霜和甘草合用后致病菌属Desulfovibrio(脱硫弧菌属)和Streptococcaceae_ukn相对丰度升高,两药合用进一步扰乱了肠道微生物稳态,且提示有引发肝脏及肠道炎症的风险。研究结果从巴豆霜传统功效及其对肠道菌群结构影响方面证实巴豆霜与甘草合用具有一定的降效增毒作用趋势,为巴豆霜临床安全用药提供了数据支持。
Based on the toxic characteristics caused by the compatibility between "Zaoji Suiyuan" and Glycyrrhizae Radix et Rhizoma, which was found in the previous studies, the expanded study was carried out on the incompatibility mechanism between Crotonis Semen Pulveratum(CT) and Glycyrrhizae Radix et Rhizoma(GU) with the diuretic effect and intestinal flora as the characteristic indexes. The results showed that GU could slow down the rapid diuretic effect of CT, which suggested a tendency of decreasing the efficacy. Both the high and low dose of CT could significantly induce the intestinal injury and change the intestinal bacteria structure of mice. Low dose CT combined with GU could significantly increase the levels of Streptococcus and Rikenellaceae_ukn. The relative abundance of Desulfovibrio and Streptococcaceae_ukn were increased after the combined application of high dose CT and GU. It also suggested that there was a risk of inflammation in the liver and intestines when combined application of these two herbs. The results revealed that the combination of CT and GU has a tendency to reduce the clinical effect and increase the toxicity from the aspects of its traditional efficacy and its effect on intestinal microflora structure, which could provide the data for the clinical use of CT.
引文
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