术芍配伍煎剂对慢传输性便秘模型大鼠肠功能及神经传递因子的影响
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摘要
目的观察术芍配伍煎剂对慢传输性便秘模型大鼠肠功能及5-羟色胺(5-HT)、降钙素基因相关肽(CGRP)、乙酰胆碱酯酶(AchE)的影响。方法将雌性Wistar大鼠随机分为空白组、模型组及术芍配伍煎剂组,每组10只。除空白组外,其余2组复制慢传输性便秘模型。造模成功后,空白组及模型组予0.9%生理盐水灌胃,术芍配伍煎剂组以6 g/kg剂量灌胃术芍配伍煎液,连续灌胃14 d。记录各组大鼠给药第2周的粪便粒数,测定粪便含水率,检测各组大鼠血清5-HT含量、结肠组织中CGRP含量及AchE活性。结果与空白组比较,模型组大鼠治疗第2周的粪便粒数明显减少,粪便含水率明显降低,血清5-HT含量、结肠组织中CGRP含量及AchE活性明显降低,2组比较差异均有统计学意义(P<0.05);与模型组比较,术芍配伍煎剂组大鼠治疗第2周的粪便粒数明显增多,粪便含水率明显升高,血清5-HT含量、结肠组织中CGRP含量及AchE活性明显升高,2组比较差异均有统计学意义(P<0.05)。结论术芍配伍煎剂可以通过调节慢传输性便秘模型大鼠的神经递质,恢复神经递质的正常释放,从而促进胃肠道的分泌和蠕动,恢复正常的排便功能。
Objective To observe the effects of Zhushao Peiwu Jianji(术芍配伍煎剂,ZPJ) on intestinal function and 5-hydroxytryptamine(5-HT),calcitonin generelated peptide(CGRP),acetylcholinesterase(AchE) of slow transit constipation in rats model.Methods Wistar female rats were randomly divided into blank group,model group and ZPJ group,with 10 rats in each.Except the blank group,the rest 2 groups duplicated the slow transit constipation models.After successful modeling,the blank group and model group were given 0.9% normal saline by gavage,the ZPJ group was given 6 g/kg dose of Zhushao Peiwu decoction by gavage,continuous administration by gavage for 14 days.The dejection amounts of the rats in each group at the 2 ndweek of administration were recorded,the fecal moisture content was determined,the serum content of 5-HT,the content of CGRP and activity of AchE in colonic tissues of the rats in each group was determined.Results Compared with the blank group,the dejection amounts of the rats in the model group at the 2 ndweek of administration decreased significantly,the fecal moisture content decreased significantly,the serum content of 5-HT,the content of CGRP and the activity of AchE in colonic tissues decreased significantly,there were statistically significant differences between the two groups(P<0.05).Compared with the model group,the dejection amounts of the rats in the ZPJ group at the 2 ndweek of administration increased significantly,the fecal moisture content increased significantly,the serum content of 5-HT and the content of CGRP and the activity of AchE in the colonic tissues increased significantly,there were statistically significant differences between the two groups(P<0.05).Conclusion ZPJ can regulate the neurotransmitters of rats with slow transit constipation,restore the normal release of neurotransmitters,thus promote the secretion and peristalsis of gastrointestinal tract,and restore the normal defecation function.
Objective To observe the effects of Zhushao Peiwu Jianji(术芍配伍煎剂,ZPJ) on intestinal function and 5-hydroxytryptamine(5-HT),calcitonin generelated peptide(CGRP),acetylcholinesterase(AchE) of slow transit constipation in rats model.Methods Wistar female rats were randomly divided into blank group,model group and ZPJ group,with 10 rats in each.Except the blank group,the rest 2 groups duplicated the slow transit constipation models.After successful modeling,the blank group and model group were given 0.9% normal saline by gavage,the ZPJ group was given 6 g/kg dose of Zhushao Peiwu decoction by gavage,continuous administration by gavage for 14 days.The dejection amounts of the rats in each group at the 2 ndweek of administration were recorded,the fecal moisture content was determined,the serum content of 5-HT,the content of CGRP and activity of AchE in colonic tissues of the rats in each group was determined.Results Compared with the blank group,the dejection amounts of the rats in the model group at the 2 ndweek of administration decreased significantly,the fecal moisture content decreased significantly,the serum content of 5-HT,the content of CGRP and the activity of AchE in colonic tissues decreased significantly,there were statistically significant differences between the two groups(P<0.05).Compared with the model group,the dejection amounts of the rats in the ZPJ group at the 2 ndweek of administration increased significantly,the fecal moisture content increased significantly,the serum content of 5-HT and the content of CGRP and the activity of AchE in the colonic tissues increased significantly,there were statistically significant differences between the two groups(P<0.05).Conclusion ZPJ can regulate the neurotransmitters of rats with slow transit constipation,restore the normal release of neurotransmitters,thus promote the secretion and peristalsis of gastrointestinal tract,and restore the normal defecation function.
引文
[1]刘兴,王文革.慢传输型便秘动物模型研究进展[J].中国中西医结合消化杂志,2013,21(10):548-551.
[2]李勇,王健,李毅平,等.芪榔合剂对慢性传输型便秘小鼠结肠SP、VIP的影响[J].世界华人消化杂志,2008,16(3):272-276.
[3]Bennett G,Thalley N J.Irritable bowel syndrome in the elderly[J].Best Pract Res Clin Gastroenterol,2012,16(8):63-76.
[4]方秀才.肠神经系统的基础与临床研究进展[J].临床消化病杂志,2009,21(2):103-104.
[5]尚精娟,唐蓉珠,徐晖,等.疏肝饮对内脏高敏感性大鼠结肠及DRG中SP和CGRP表达的影响[J].辽宁中医杂志,2014,41(6):1287-1290.
[6]刘仍海,刘薇,王蓬文,等.温阳健脾法对慢传输型便秘模型大鼠结肠神经递质影响的研究[J].北京中医药,2013,32(9):698-700.
[7]Terence K Smith,Kyu Joo Park,Grant W Hennig. Colonic migrating motor complexes,high amplitude propagating contractions,neural reflexes and the importance of neuronal and mucosal serotonin[J]. J Neurogastroenterol Motil,2014,20(4):423-446.
[8]孙亚楠,王世军,王晓燕.电针对便秘型肠易激综合征模型大鼠血浆降钙素基因相关肽和P物质表达的影响[J].国际中医中药杂志,2013,35(5):413-415.
[9]吴翰桂,朱玲,马勇军.白术对小鼠胃肠道活动的影响的实验研究[J].江苏中医药,2005,26(11):66-67.
[10]陈嘉屿,刘德科,吴红梅,等.生白术对高原缺氧大鼠小肠运动和5-HT4受体的影响[J].胃肠病学,2015,20(3):138-142.
[11]陶黎敏,祝明珠,陈火根,等.不同剂量白芍对慢性功能性便秘的治疗作用[J].中国肛肠病杂志,2008,28(8):25-26.
[2]李勇,王健,李毅平,等.芪榔合剂对慢性传输型便秘小鼠结肠SP、VIP的影响[J].世界华人消化杂志,2008,16(3):272-276.
[3]Bennett G,Thalley N J.Irritable bowel syndrome in the elderly[J].Best Pract Res Clin Gastroenterol,2012,16(8):63-76.
[4]方秀才.肠神经系统的基础与临床研究进展[J].临床消化病杂志,2009,21(2):103-104.
[5]尚精娟,唐蓉珠,徐晖,等.疏肝饮对内脏高敏感性大鼠结肠及DRG中SP和CGRP表达的影响[J].辽宁中医杂志,2014,41(6):1287-1290.
[6]刘仍海,刘薇,王蓬文,等.温阳健脾法对慢传输型便秘模型大鼠结肠神经递质影响的研究[J].北京中医药,2013,32(9):698-700.
[7]Terence K Smith,Kyu Joo Park,Grant W Hennig. Colonic migrating motor complexes,high amplitude propagating contractions,neural reflexes and the importance of neuronal and mucosal serotonin[J]. J Neurogastroenterol Motil,2014,20(4):423-446.
[8]孙亚楠,王世军,王晓燕.电针对便秘型肠易激综合征模型大鼠血浆降钙素基因相关肽和P物质表达的影响[J].国际中医中药杂志,2013,35(5):413-415.
[9]吴翰桂,朱玲,马勇军.白术对小鼠胃肠道活动的影响的实验研究[J].江苏中医药,2005,26(11):66-67.
[10]陈嘉屿,刘德科,吴红梅,等.生白术对高原缺氧大鼠小肠运动和5-HT4受体的影响[J].胃肠病学,2015,20(3):138-142.
[11]陶黎敏,祝明珠,陈火根,等.不同剂量白芍对慢性功能性便秘的治疗作用[J].中国肛肠病杂志,2008,28(8):25-26.