饲料铜水平对斑节对虾血细胞中酚氧化酶原系统相关基因表达的影响
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摘要
以不同铜离子添加水平(0 mg·kg-1、10 mg·kg-1、25 mg·kg-1、40 mg·kg-1、55 mg·kg-1和110 mg·kg-1)的饲料喂养斑节对虾Penaeus monodon 8周,测定对虾血细胞中酚氧化酶原系统相关基因(pro PO1、pro PO2、PE、PPAF和BGBP)表达水平的变化。结果显示,与对照组相比,当铜添加水平为40~55 mg·kg-1时,proPO1的表达量显著上升;当铜添加水平为25~55 mg·kg-1时,pro PO2、PE和PPAF的表达量显著上升;铜添加水平对BGBP的表达量没有显著影响。血清PO活力也在铜添加水平为25~55 mg·kg-1时显著提高。这些结果表明,饲料中添加一定量的铜离子可诱导pro PO1、pro PO2、PE和PPAF表达量的上调,从而提高血清PO活力。根据上述结果,针对提高酚氧化酶原系统免疫功能,在本基础饲料配方中适宜的铜添加水平为25~55 mg·kg-1。
An 8-week feeding trial was conducted to investigate the effects of different concentrations of dietary copper( 0,10,25,40,55 and 110 mg supplemental Cu / kg diet) on the expression of prophenoloxidase system-related genes( proPO1,pro PO2,PE,PPAF and BGBP) in tiger shrimp Penaeus monodon haemocyte. The results showed that,compared to the control,expression of pro PO1 increased significantly in shrimp fed with diets containing 40- 55 mg·kg- 1of Cu. Expression levels of pro PO2,PE and PPAF increased significantly in shrimp fed with diets containing 25- 55 mg· kg- 1of Cu. Cu supplementation did not affect the expression of BGBP. Serum PO activity also increased in shrimp fed with diets supplemented with 25- 55 mg·kg- 1of Cu. These results indicated that adequate supplemental Cu would induce the gene expression of pro PO1,pro PO2,PE and PPAF,and then improve the serum PO activity. In conclusion,for the improvement of prophenoloxidase system,the adequate supplemental Cu concentration was 25- 55 mg·kg- 1for the present basal diet.
An 8-week feeding trial was conducted to investigate the effects of different concentrations of dietary copper( 0,10,25,40,55 and 110 mg supplemental Cu / kg diet) on the expression of prophenoloxidase system-related genes( proPO1,pro PO2,PE,PPAF and BGBP) in tiger shrimp Penaeus monodon haemocyte. The results showed that,compared to the control,expression of pro PO1 increased significantly in shrimp fed with diets containing 40- 55 mg·kg- 1of Cu. Expression levels of pro PO2,PE and PPAF increased significantly in shrimp fed with diets containing 25- 55 mg· kg- 1of Cu. Cu supplementation did not affect the expression of BGBP. Serum PO activity also increased in shrimp fed with diets supplemented with 25- 55 mg·kg- 1of Cu. These results indicated that adequate supplemental Cu would induce the gene expression of pro PO1,pro PO2,PE and PPAF,and then improve the serum PO activity. In conclusion,for the improvement of prophenoloxidase system,the adequate supplemental Cu concentration was 25- 55 mg·kg- 1for the present basal diet.
引文
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周萌,王安利,曹俊明.2010.饲料中不同形式的铜及添加量对凡纳滨对虾(Paneaus vannamei)生长性能、血清铜蓝蛋白和生长激素水平的影响[J].海洋与湖沼,41(4):577-582.
Huang JW,Yang Y,Wang AL.2010.Reconsideration of phenoloxidaseactivity determination in white shrimp Litopenaeus vannamei[J].Fish&Shellfish Immunology,28:240-244.
Lee MH,Shiau SY.2002.Dietary copper requirement of juvenile grassshrimp,Penaeus monodon,and effects on non-specific immune responses[J].Fish&Shellfish Immunology,13:259-270.
Liu CH,Yeh SP,Kuo CM,et al.2006.The effect of sodium alginate onthe immune response of tiger shrimp via dietary administration:Activity and gene transcription[J].Fish&Shellfish Immunology,21:442-452.
Wang YC,Chang PS,Chen HY.2008.Differential time-series expression of immune-related genes of Pacific white shrimp Litopenaeus vannamei in response to dietary inclusion ofβ-1,3-glucan[J].Fish&Shellfish Immunology,24:113-121.
郭慧,冼健安,毕建柱,等.2013.虾类免疫因子的研究进展[J].饲料工业,34(22):42-46.
郭腾飞,黄旭雄,苏明,等.2012.饲料中铜水平对凡纳滨对虾免疫相关基因表达和抗菌能力的影响[J].水生生物学报,36(5):[5]809-816.
郭志勋,陈毕生,徐力文,等.2003.饲料铜的添加量对南美白对虾生长、血液免疫因子及组织铜的影响[J].中国水产科学,10(6):[7]526-528.
刘发义,梁德海,孙凤,等.1990.饵料中的铜对中国对虾的影响[J].海洋与湖沼,21(5):404-410.
冼健安,苗玉涛,叶超霞,等.2013.饲料铜含量对亚硝酸盐胁迫下斑节对虾血细胞的影响[J].水生态学杂志,34(5):71-75.
冼健安,王安利.2013.饲料中铜离子对斑节对虾血细胞组成、活性和免疫功能的影响[J].海洋科学,37(2):40-46.
阳会军,谭北平,方怀义.2001.饲料中添加蛋氨酸铜和硫酸铜对斑节对虾生长和存话的影响[J].饲料工业,22(10):15-16.
周萌,王安利,曹俊明.2010.饲料中不同形式的铜及添加量对凡纳滨对虾(Paneaus vannamei)生长性能、血清铜蓝蛋白和生长激素水平的影响[J].海洋与湖沼,41(4):577-582.
Huang JW,Yang Y,Wang AL.2010.Reconsideration of phenoloxidaseactivity determination in white shrimp Litopenaeus vannamei[J].Fish&Shellfish Immunology,28:240-244.
Lee MH,Shiau SY.2002.Dietary copper requirement of juvenile grassshrimp,Penaeus monodon,and effects on non-specific immune responses[J].Fish&Shellfish Immunology,13:259-270.
Liu CH,Yeh SP,Kuo CM,et al.2006.The effect of sodium alginate onthe immune response of tiger shrimp via dietary administration:Activity and gene transcription[J].Fish&Shellfish Immunology,21:442-452.
Wang YC,Chang PS,Chen HY.2008.Differential time-series expression of immune-related genes of Pacific white shrimp Litopenaeus vannamei in response to dietary inclusion ofβ-1,3-glucan[J].Fish&Shellfish Immunology,24:113-121.