碳源诱导嗜热脂肪芽孢杆菌生产淀粉分支酶的研究
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摘要
利用嗜热脂肪芽孢杆菌(Bacillus stearothermophilus)生产淀粉分支酶,研究不同碳源种类及浓度对嗜热脂肪芽孢杆菌产酶性能的影响。结果表明,以0.20%麦芽糊精为碳源时,淀粉分支酶的酶活最高,达20.02 U/m L,是其他碳源的2～10倍。采用最优培养基在小试试验的基础上进行了扩大培养中试试验,淀粉分支酶酶活稳定,为19.87 U/m L,是小试试验的1.1倍。
Bacillus stearothermophilus was used to produce starch branching enzyme. The effect of different carbon source types and concentrations on enzyme production performance of B. stearothermophilus was studied. The results showed that when the carbon source in the fermentation medium was maltodextrin 0.20%, the starch branching enzyme activity was the highest, and reached 20.02 U/ml, which was 2 to 10 times that of other media. On the basis of the lab-scale experiment, the pilot-scale experiment was carried out, and the starch branching enzyme activity was stable and was 19.87 U/ml, which was 1.1 times that of the lab-scale experiment.
Bacillus stearothermophilus was used to produce starch branching enzyme. The effect of different carbon source types and concentrations on enzyme production performance of B. stearothermophilus was studied. The results showed that when the carbon source in the fermentation medium was maltodextrin 0.20%, the starch branching enzyme activity was the highest, and reached 20.02 U/ml, which was 2 to 10 times that of other media. On the basis of the lab-scale experiment, the pilot-scale experiment was carried out, and the starch branching enzyme activity was stable and was 19.87 U/ml, which was 1.1 times that of the lab-scale experiment.
引文
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[27]DUMEZ S,WATTEBLED F,DAUVILLEE D,et al.Mutants of arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation[J].Plant Cell,2006,18(10):2694-2709.
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[29]KIM E J,RYU S I,BAE H A,et al.Biochemical characterisation of a glycogen branching enzyme from Streptococcus mutans:enzymatic modification of starch[J].Food Chem,2008,110(4):979-984.
[30]KIM E J,RYU S I,BAE H A,et al.Biochemical characterisation of a glycogen branching enzyme from Streptococcus mutans:enzymatic modification of starch[J].Food Chem,2008,110(4):979-984.
[31]VAN DER VLIST J,REIXACH M P,VAN DER MAAREL M,et al.Synthesis of branched polyglucans by the tandem action of potato phosphorylase and Deinococcus geothermalis glycogen branching enzyme[J].Macromol Rapid Commun,2008,29(15):1293-1297.
[32]VAN DER VLIST J,FABER M,LOEN L,et al.Synthesis of hyperbranched glycoconjugates by the combined action of potato phosphorylase and glycogen branching enzyme from Deinococcus geothermalis[J].Polymers,2012,4(1):674-690.
[33]ROUSSEL X,LANCELON-PIN C,VIKSO-NIELSEN A,et al.Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis[J].Biochim Biophys Acta,2013,1830(1):2167-2177.
[34]JO H J,PARK S,JEONG H G,et al.Vibrio vulnificus glycogen branching enzyme preferentially transfers very short chains:N1 domain determines the chain length transferred[J].FEBS Lett,2015,589(10):1089-1094.
[35]SUZUKI E,SUZUKI R.Distribution of glucan-branching enzymes among prokaryotes[J].Cell Mol Life Sci,2016,73(14):2643-2660.
[36]PALOMO M,PIJNING T,BOOIMAN T,et al.Thermus thermophilus glycoside hydrolase family 57 branching enzyme crystal structure,mechanism of action,and products formed[J].J Biol Chem,2011,286(5):3520-3530.
[37]ABAD M C,BINDERUP K,RIOS-STEINER J,et al.The X-ray crystallographic structure of Escherichia coli branching enzyme[J].J Biol Chem,2002,277(44):42164-42170.
[38]CHRONAKIS I S.On the molecular characteristics,compositional properties,and structural-functional mechanisms of maltodextrins:a review[J].Food Sci Nutr,1998,38(7):599-637.
[2]GUZMAN MALDONADO H,PAREDES LOPEZ O.Amylolytic enzymes and products derived from starch:A review[J].Crit Rev Food Sci Nutr,1995,35(5):373-403.
[3]KEISER J E.Starch:its morphology,chemistry and industrial applications[J].Abstr Pap Am Chem Soc,2003,225:U982-U982.
[4]JOBLING S.Improving starch for food and industrial applications[J].Curr Opin Plant Biol,2004,7(2):210-218.
[5]ZHAO S S,DUFOUR D,SANCHEZ T,et al.Development of waxy cassava with different biological and physico-chemical characteristics of starches for industrial applications[J].Biotechnol Bioeng,2011,108(8):1925-1935.
[6]DASTIDAR T G,NETRAVALI A N.Improving resin and film forming properties of native starches by chemical and physical modification[J].JBiobased Mater Bioenergy,2012,6(1):1-24.
[7]WALISZEWSKI K N,APARICIO M A,BELLO L A,et al.Changes of banana starch by chemical and physical modification[J].Carbohydr Polym,2003,52(3):237-242.
[8]DIN Z U,XIONG H G,FEI P.Physical and chemical modification of starches:A review[J].Crit Rev Food Sci Nutr,2017,57(12):2691-2705.
[9]ASHOGBON A O,AKINTAYO E T.Recent trend in the physical and chemical modification of starches from different botanical sources:a review[J].Starch-Starke,2014,66(1-2):41-57.
[10]RAIGOND P,EZEKIEL R,RAIGOND B.Resistant starch in food:a review[J].J Sci Food Agric,2015,95(10):1968-1978.
[11]LI W W,LI C M,GU Z B,et al.Relationship between structure and retrogradation properties of corn starch treated with 1,4-alpha-glucan branching enzyme[J].Food Hydrocoll,2016,52:868-875.
[12]LEGLER G.Mechanism of enzymatic glycoside hydrolysis and of glycosyl transfer by glycosidases and glycosyltransferases[J].Carbohydr Res,1993,250(1):R7-R20.
[13]SHINOHARA M L,IHARA M,ABO M,et al.A novel thermostable branching enzyme from an extremely thermophilic bacterial species,Rhodothermus obamensis[J].Appl Microbiol Biotechnol,2001,57(5-6):653-659.
[14]HAYASHI M,SUZUKI R,COLLEONI C,et al.Bound substrate in the structure of cyanobacterial branching enzyme supports a new mechanistic model[J].J Biol Chem,2017,292(13):5465-5475.
[15]TETLOW I J,EMES M J.A review of starch-branching enzymes and their role in amylopectin biosynthesis[J].Iubmb Life,2014,66(8):546-558.
[16]SUZUKI E,SUZUKI R.Distribution of glucan-branching enzymes among prokaryotes[J].Cell Mol Life Sci,2016,73(14):2643-2660.
[17]CHOI J H,LEE S Y.Secretory and extracellular production of recombinant proteins using Escherichia coli[J].Appl Microbiol Biotechnol,2004,64(5):625-635.
[18]KURIKI T,STEWART D C,Preiss J.Construction of chimeric enzymes out of maize endosperm branching enzymes I and II:activity and properties[J].J Biol Chem,1997,272(46):28999-29004.
[19]BALL S G,MORELL M K.From bacterial glycogen to starch:understanding the biogenesis of the plant starch granule[J].Annu Rev Plant Biol,2003,54:207-233.
[20]TETLOW I J,WAIT R,LU Z,et al.Protein phosphorylation in amyloplasts regulates starch branching enzyme activity and protein-protein interactions[J].Plant Cell,2004,16(3):694-708.
[21]DUMEZ S,WATTEBLED F,DAUVILLEE D,et al.Mutants of arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation[J].Plant Cell,2006,18(10):2694-2709.
[22]李太贵,沈波,陈能,等.Q酶对水稻籽粒垩白形成的影响[J].作物学报,1997,23(3):338-344.
[23]SUZUKI H,HASHIDA K,NGUYEN K H,et al.Efficacy of intratympanic steroid administration on idiopathic sudden sensorineural hearing loss in comparison with hyperbaric oxygen therapy[J].Laryngoscope,2012,122(5):1154-1157.
[24]阮森林.酸性α-淀粉酶产生菌的筛选及其酶学性质研究[D].郑州:河南农业大学,2008.
[25]陈晨.耐高温α-淀粉酶菌株的筛选、发酵条件优化及酶基因的克隆[D].长沙:中南大学,2009.
[26]TETLOW I J,WAIT R,LU Z,et al.Protein phosphorylation in amyloplasts regulates starch branching enzyme activity and protein-protein interactions[J].Plant Cell,2004,16(3):694-708.
[27]DUMEZ S,WATTEBLED F,DAUVILLEE D,et al.Mutants of arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation[J].Plant Cell,2006,18(10):2694-2709.
[28]RUMBAK E,RAWLINGS D E,LINDSEY G G,et al.Characterization of the butyrivibrio-fibrisolvens Glgb gene,which encodes a glycogenbranching enzyme with starch-clearing activity[J].J Bacteriol,1991,173(21):6732-6741.
[29]KIM E J,RYU S I,BAE H A,et al.Biochemical characterisation of a glycogen branching enzyme from Streptococcus mutans:enzymatic modification of starch[J].Food Chem,2008,110(4):979-984.
[30]KIM E J,RYU S I,BAE H A,et al.Biochemical characterisation of a glycogen branching enzyme from Streptococcus mutans:enzymatic modification of starch[J].Food Chem,2008,110(4):979-984.
[31]VAN DER VLIST J,REIXACH M P,VAN DER MAAREL M,et al.Synthesis of branched polyglucans by the tandem action of potato phosphorylase and Deinococcus geothermalis glycogen branching enzyme[J].Macromol Rapid Commun,2008,29(15):1293-1297.
[32]VAN DER VLIST J,FABER M,LOEN L,et al.Synthesis of hyperbranched glycoconjugates by the combined action of potato phosphorylase and glycogen branching enzyme from Deinococcus geothermalis[J].Polymers,2012,4(1):674-690.
[33]ROUSSEL X,LANCELON-PIN C,VIKSO-NIELSEN A,et al.Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis[J].Biochim Biophys Acta,2013,1830(1):2167-2177.
[34]JO H J,PARK S,JEONG H G,et al.Vibrio vulnificus glycogen branching enzyme preferentially transfers very short chains:N1 domain determines the chain length transferred[J].FEBS Lett,2015,589(10):1089-1094.
[35]SUZUKI E,SUZUKI R.Distribution of glucan-branching enzymes among prokaryotes[J].Cell Mol Life Sci,2016,73(14):2643-2660.
[36]PALOMO M,PIJNING T,BOOIMAN T,et al.Thermus thermophilus glycoside hydrolase family 57 branching enzyme crystal structure,mechanism of action,and products formed[J].J Biol Chem,2011,286(5):3520-3530.
[37]ABAD M C,BINDERUP K,RIOS-STEINER J,et al.The X-ray crystallographic structure of Escherichia coli branching enzyme[J].J Biol Chem,2002,277(44):42164-42170.
[38]CHRONAKIS I S.On the molecular characteristics,compositional properties,and structural-functional mechanisms of maltodextrins:a review[J].Food Sci Nutr,1998,38(7):599-637.