HPLC同时测定退黄保肝胶囊中3种成分的含量
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摘要
目的建立HPLC测定退黄保肝胶囊中绿原酸、柚皮苷及新橙皮苷含量的方法。方法色谱柱:InertSustain C_(18) Superb(4.6 mm×250 mm,5μm);流动相:乙腈-0.2%磷酸溶液(15:85);流速:1.0 ml/min;检测波长283 nm;柱温:35℃。结果 3种成分的线性关系均良好,绿原酸回归方程为Y=2×10~6X-523,r=0.9999,柚皮苷回归方程为Y=2×10~6X+388,r=0.9999,新橙皮苷回归方程为Y=2×10~6X+436,r=0.9996,回收率分别99.0%,99.3%,100.1%,RSD均低于2.0%。结论本方法简单快速,适用于测定退黄保肝胶囊中的绿原酸、柚皮苷和新橙皮苷的含量。
Objective To establish a method for simultaneous determination of chlorogenic acid, naringin and neohesperidin in Tuihuangbaogan Capsules by HPLC. Methods InertSustain C_(18) Superb(4.6 mm×250 mm, 5 μm)was adopted, using acetonitrile-0.2 % phosphoric acid solution(15:85) as mobile phase, with a ?ow rate of 1.0 ml/min. The detection wavelength was set at 283 nm; the column temperature was 35 ℃. Results The linear relationships of the three components were good. The regression equation of chlorogenic acid was Y=2×10~6 X-523, r=0.9999;the regression equation of naringin was Y=2×10~6 X+388, r=0.9999; and the regression equation of neohesperidin was Y=2×10~6 X+436, r=0.9996. The recoveries were 99.0 %, 99.3 % and 100.1 %, respectively. The RSDs were lower than 2.0 %. Conclusion The method is simple and rapid, and can be used to determine the contents of chlorogenic acid,naringin and neohesperidin in the Tuihuangbaogan Capsules.
Objective To establish a method for simultaneous determination of chlorogenic acid, naringin and neohesperidin in Tuihuangbaogan Capsules by HPLC. Methods InertSustain C_(18) Superb(4.6 mm×250 mm, 5 μm)was adopted, using acetonitrile-0.2 % phosphoric acid solution(15:85) as mobile phase, with a ?ow rate of 1.0 ml/min. The detection wavelength was set at 283 nm; the column temperature was 35 ℃. Results The linear relationships of the three components were good. The regression equation of chlorogenic acid was Y=2×10~6 X-523, r=0.9999;the regression equation of naringin was Y=2×10~6 X+388, r=0.9999; and the regression equation of neohesperidin was Y=2×10~6 X+436, r=0.9996. The recoveries were 99.0 %, 99.3 % and 100.1 %, respectively. The RSDs were lower than 2.0 %. Conclusion The method is simple and rapid, and can be used to determine the contents of chlorogenic acid,naringin and neohesperidin in the Tuihuangbaogan Capsules.
引文
[1]国家药典委员会.中华人民共和国药典2015年版(一部)[S].北京:中国医药科技出社, 2015:221, 239, 246.
[2]那袭雪,张文涛,谈远锋,等.绿原酸及其异构体药理作用及不良反应研究进展[J].辽宁中医药大学学报, 2018, 20(3):140-144.
[3]章斌,金剑,金芝贵,等.枳壳的药理作用与临床应用进展[J].医药导报, 2013, 32(11):1462-1464.
[4]姜华,郭敏,徐世红,等. HPLC法测定忍冬藤痛风颗粒中绿原酸的含量[J].西部中医药, 2017, 30(4):25-27.
[5]宋剑锋,冯敬骞,胡建华,等.HPLC法同时测定常山胡柚花中柚皮苷、橙皮苷和新橙皮苷的量[J].中草药, 2014, 45(6):854-856.
[6]彭玲娜,丁野,孙辉,等. HPLC法同时测定胃痛丸中柚皮苷、新橙皮苷的含量[J].中国药师, 2017, 20(6):1112-1114.
[7]任贻军,杨远荣,李华荣,等.HPLC法同时测定补脾养胃丸中柚皮苷和新橙皮苷的含量[J].中医药导报, 2014, 20(14):47-48,51.
[8]杨世艳,何兵,张燕.HPLC同时测定银黄颗粒中7种有机酸及4种黄酮类成分[J].中草药, 2013, 44(3):301-304.
[9]桂蜀华,陈军,王林,等. HPLC法同时测定银翘柴桂颗粒中绿原酸、芍药苷和黄芩苷[J].中成药, 2011, 33(7):1175-1178.
[2]那袭雪,张文涛,谈远锋,等.绿原酸及其异构体药理作用及不良反应研究进展[J].辽宁中医药大学学报, 2018, 20(3):140-144.
[3]章斌,金剑,金芝贵,等.枳壳的药理作用与临床应用进展[J].医药导报, 2013, 32(11):1462-1464.
[4]姜华,郭敏,徐世红,等. HPLC法测定忍冬藤痛风颗粒中绿原酸的含量[J].西部中医药, 2017, 30(4):25-27.
[5]宋剑锋,冯敬骞,胡建华,等.HPLC法同时测定常山胡柚花中柚皮苷、橙皮苷和新橙皮苷的量[J].中草药, 2014, 45(6):854-856.
[6]彭玲娜,丁野,孙辉,等. HPLC法同时测定胃痛丸中柚皮苷、新橙皮苷的含量[J].中国药师, 2017, 20(6):1112-1114.
[7]任贻军,杨远荣,李华荣,等.HPLC法同时测定补脾养胃丸中柚皮苷和新橙皮苷的含量[J].中医药导报, 2014, 20(14):47-48,51.
[8]杨世艳,何兵,张燕.HPLC同时测定银黄颗粒中7种有机酸及4种黄酮类成分[J].中草药, 2013, 44(3):301-304.
[9]桂蜀华,陈军,王林,等. HPLC法同时测定银翘柴桂颗粒中绿原酸、芍药苷和黄芩苷[J].中成药, 2011, 33(7):1175-1178.