预处理及表面活性剂对米曲霉固体发酵产纤维素酶影响的研究
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摘要
本试验以米曲霉HML366为出发菌株,研究纤维素预处理及化学表面活性剂Tween 80对米曲霉固体发酵产纤维素酶的影响。结果表明:加入不同浓度的Tween 80对预处理过的蔗渣滤纸酶活的影响较为显著,蔗渣经5%氨水、5%乙酸、10%乙酸处理后,添加0.8%Tween 80的滤纸酶活分别可以达到6.6、6.3 U/mL和6.1 U/mL。当Tween 80浓度增加到1.2%浓度时,滤纸酶活有所下降。预处理过的蔗渣及添加Tween 80经发酵后,其发酵液中蛋白质含量较高,酶活较高。
The effects of cellulose pretreatment and chemical surfactant Tween 80 on cellulase production by Aspergillus oryzae HML366 were studied. Different concentrations of Tween 80 had significant effect on the enzyme activity of pretreated bagasse filter paper. After pretreated bagasse with 5% ammonia,5% acetic acid and 10% acetic acid,the enzyme activity of filter paper with 0.8% Tween 80 could reach 6.6,6.3 U/mL and 6.1 U/mL,respectively.When the concentration of Tween 80 increased to 1.2%,the activity of filter paper decreased. After pretreated bagasse and adding Tween 80,the protein content and enzyme activity in the fermentation broth were higher.
The effects of cellulose pretreatment and chemical surfactant Tween 80 on cellulase production by Aspergillus oryzae HML366 were studied. Different concentrations of Tween 80 had significant effect on the enzyme activity of pretreated bagasse filter paper. After pretreated bagasse with 5% ammonia,5% acetic acid and 10% acetic acid,the enzyme activity of filter paper with 0.8% Tween 80 could reach 6.6,6.3 U/mL and 6.1 U/mL,respectively.When the concentration of Tween 80 increased to 1.2%,the activity of filter paper decreased. After pretreated bagasse and adding Tween 80,the protein content and enzyme activity in the fermentation broth were higher.
引文
[1]管斌,丁友.还原糖测定方法的规范[J].无锡轻工大学学报,1999,18(3):74~79.
[2]赫志军,李忠兴.青霉NXP25纤维素的生产及性质[J].微生物学通报,2002,29(1):64~66.
[3]李建武,萧能,余瑞元,等.生物化学实验原理和方法[M].北京:北京大学出版社,1994:60~176.
[4]林英,秦萍,杜志强,等.产纤维素酶绿色木酶产酶优化[J].安徽农业科学,2006,34(11):2312~2314.
[5]沈萍,陈向东.微生物学实验.第四版[M].北京:高等教育出版社,2007.241~244.
[6]王超,章超桦.酶解纤维素类物质生产燃料酒精的研究进展[J].纤维素科学术,2003,11(4):52~59.
[7]王丹,林建强,张萧,等.直接生物转化纤维素类资源生产燃料乙醇的研究进展[J].山东农业大学学报,2002,33(4):525~529.
[8]姚兰,赵建,谢益民,等.木质素结构以及表面活性剂对木质素吸附纤维素酶的影响[J].化工学报,2012,63(8):2612~2616.
[9]赵春玲,王秀霞,李琼.3,5-二硝基水杨酸比色法测定废烟草中总糖[J].湖北工业大学学报,2006,21(6):62~65.
[10]周妍,赵雪冰,刘德华.非离子型表面活性剂对木质纤维素酶催化水解的影响及机理[J].化学进展,2015,27(11):1555~1565.
[11]张树政.酶制剂工业[M].北京:科学出版社,1984.595~623.
[12] Ghose T K.Measurement of cellulose activities[J].Pure Appl Chem,1987,59(2):257~626.
[13] Mandels M,Andreott R E,Rochei C H.Measurement of saccharifying cellulose[J].Biotechnology&Bioengineering Symposium,1976,6:21~33.
[14] Mohammed I Y,Abakr Y A,Mokaya R.Valorisation of adzuki bean waste to biofuel precursors via pyrolysis:kinetics,product distribution and characterisation[J].Biomass Conversion and Biorefinery,2018,8(3):699~710.
[15] Smettem K.Charaeterization of Water Entry into a Soil with a Contrasting Textural Class:Spatial Variability of Infiltration Parameters and Influence of Maero Porosity[J].Soil Sci,1987,144(3):167~174.
[2]赫志军,李忠兴.青霉NXP25纤维素的生产及性质[J].微生物学通报,2002,29(1):64~66.
[3]李建武,萧能,余瑞元,等.生物化学实验原理和方法[M].北京:北京大学出版社,1994:60~176.
[4]林英,秦萍,杜志强,等.产纤维素酶绿色木酶产酶优化[J].安徽农业科学,2006,34(11):2312~2314.
[5]沈萍,陈向东.微生物学实验.第四版[M].北京:高等教育出版社,2007.241~244.
[6]王超,章超桦.酶解纤维素类物质生产燃料酒精的研究进展[J].纤维素科学术,2003,11(4):52~59.
[7]王丹,林建强,张萧,等.直接生物转化纤维素类资源生产燃料乙醇的研究进展[J].山东农业大学学报,2002,33(4):525~529.
[8]姚兰,赵建,谢益民,等.木质素结构以及表面活性剂对木质素吸附纤维素酶的影响[J].化工学报,2012,63(8):2612~2616.
[9]赵春玲,王秀霞,李琼.3,5-二硝基水杨酸比色法测定废烟草中总糖[J].湖北工业大学学报,2006,21(6):62~65.
[10]周妍,赵雪冰,刘德华.非离子型表面活性剂对木质纤维素酶催化水解的影响及机理[J].化学进展,2015,27(11):1555~1565.
[11]张树政.酶制剂工业[M].北京:科学出版社,1984.595~623.
[12] Ghose T K.Measurement of cellulose activities[J].Pure Appl Chem,1987,59(2):257~626.
[13] Mandels M,Andreott R E,Rochei C H.Measurement of saccharifying cellulose[J].Biotechnology&Bioengineering Symposium,1976,6:21~33.
[14] Mohammed I Y,Abakr Y A,Mokaya R.Valorisation of adzuki bean waste to biofuel precursors via pyrolysis:kinetics,product distribution and characterisation[J].Biomass Conversion and Biorefinery,2018,8(3):699~710.
[15] Smettem K.Charaeterization of Water Entry into a Soil with a Contrasting Textural Class:Spatial Variability of Infiltration Parameters and Influence of Maero Porosity[J].Soil Sci,1987,144(3):167~174.