绞股蓝皂苷-硒配合物对酪氨酸酶的活性影响及动力学分析
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摘要
为考察绞股蓝皂苷及其硒配合物对酪氨酸酶的动力学参数和作用机理。本研究采用体外酶促反应,以L-酪氨酸和L-DOPA为底物,模拟了酪氨酸酶单酚和二酚酶的体外催化氧化过程。绞股蓝总皂苷在50%、70%乙醇洗脱段和50%、70%乙醇洗脱绞股蓝皂苷-硒配合物在酪氨酸酶上的Ki值分别为1. 533、1. 767、1. 312和1. 210 mmol/L。Ki值越低,对酪氨酸酶的抑制作用越强,单酚酶的氧化阶段越快,表明硒元素显著提高了绞股蓝皂苷对酪氨酸酶的抑制作用。酶反应动力学分析表明,四种绞股蓝皂苷及其硒配合物对酪氨酸的抑制作用均为混合竞争抑制。其独特的药理化学特性为绞股蓝及硒系美白化妆品的进一步研究开发提供了理论依据和参考。
By in vitro enzymatic reaction,the kinetic parameters and mechanism of Gypenosides on tyrosinase were compared with their selenium-complexes. L-tyrosine and L-DOPA were used as substrates to simulate the catalytic oxidation process of tyrosinase monophenol and diphenol enzyme in vitro. The Ki valures of gypenoside on 50%,70% ethanol elution segments and gypenoside-selenium complexes on 50%,70% ethanol eluting gypenoside on tyrosinase were 1. 533,1. 767,1. 312,and1. 210 mmol/L respectively. The lower Ki value indicated that higher concentrations of Selenium could enhance higher inhibitory effect of Gynostemma pentaphyllum on tyrosinase,otherwise,the oxidation stage of monophenolase was a quick step. The kinetic analysis of enzyme reaction showed that the inhibitory effects of four gypenosides and its selenium-complexes on tyrosine were all mixed-competitive inhibition. The specific pharmacological and chemical characteristics provided a theoretical basis and reference for further research and development of Gynostemma pentaphyllum and selenium related whitening cosmetics.
By in vitro enzymatic reaction,the kinetic parameters and mechanism of Gypenosides on tyrosinase were compared with their selenium-complexes. L-tyrosine and L-DOPA were used as substrates to simulate the catalytic oxidation process of tyrosinase monophenol and diphenol enzyme in vitro. The Ki valures of gypenoside on 50%,70% ethanol elution segments and gypenoside-selenium complexes on 50%,70% ethanol eluting gypenoside on tyrosinase were 1. 533,1. 767,1. 312,and1. 210 mmol/L respectively. The lower Ki value indicated that higher concentrations of Selenium could enhance higher inhibitory effect of Gynostemma pentaphyllum on tyrosinase,otherwise,the oxidation stage of monophenolase was a quick step. The kinetic analysis of enzyme reaction showed that the inhibitory effects of four gypenosides and its selenium-complexes on tyrosine were all mixed-competitive inhibition. The specific pharmacological and chemical characteristics provided a theoretical basis and reference for further research and development of Gynostemma pentaphyllum and selenium related whitening cosmetics.
引文
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2 Chen QX,Song KK.Research progress in tyrosinase[J].JXiamen Univ:Nat Sci(厦门大学学报:自科版),2006,45:731-737.
3 Hong XY.Extraction,isolation and purification of Gypenosides and their antioxidation effects[D].Guangzhou:South China University of Technology(华南理工大学),2012.
4 Chen SK.Classification system and distribution of Gynostemma[J].Acta Phytotaxon Sin(植物分类学报),1995,33:403-410.
5 Bao FX,Tao LX,Zhang HY.Research progress on pharmacological effects of active ingredients of Gynostemma pentaphyllum[J].Chin J New Drug and Clin Med(中国新药与临床医学杂志),2018,37:11-17.
6 Guo JJ,Ren DY,Li Z.Protective effect of Gynostemma pentaphyllum saponins on vascular endothelial damage in mice induced by high choline diet[J].Food Bio J(食品与生物技术杂志),2017,36:1131-1136.
7 Qu LZ,Yang SJ,Zhuo S,et al.Role of trace essential elements in selenium[J].Chin Agr Bul(中国农业通报),2010,26(7):94-97.
8 Chen LH,Liu X,Ming YL,et al.Inhibition and antioxidant effect of extracts from leaves of leaves to tyrosinase[J].JXiamen Univ:Nat Sci(厦门大学学报:自科版),2012,51:410-414.
9 Wang J,Xu DP.Tyrosinase-Inhibition components from buckwheat bee pollen extract[J].Nat Prod Res Dev(天然产物研究与开发),2017,29:101-105.
10 Mu Y.Purification and inhibition mechanism of a thousand day red tyrosinase inhibitor[D].Guangzhou:South China U-niversity of Technology(华南理工大学),2012.
11 Stadtman TC.Seleaoproteins-tracing the role of a trace element in protein function[J].P Biol,2005,3:2077-2079.
12 Su TJ,Wu C,Liu Q,et al.Tyrosinase inhibition activity of the fermented product of endophytic fungi isolated from Fritillariae cirrhosae Bulbus[J].Nat Prod Res Dev(天然产物研究与开发),2017,29:1368-1373.
13 Valentina RN,Tom HWH,Van HT,et al.Chemistry and pharmacology of Gynostemma pentaphyllum[J].Phyto Chem Rev,2005,4:197-219.
14 Carey AM,Lombi E,Donner E,et al.A review of recent developments in the speciation and location of arsenic and selenium in rice grain[J].Anal Bioanal Chem,2012,402:3379-3379.
15 Kang SM,Heo SJ,Kim KN,et al.Isolation and identification of new compound 2,7-phloroglucinol-6,6-bieckol from brown algae eckloniacava and its antioxidant effect[J].J Func Foods,2012,4:158-166.
16 Feng YC,Studies on tyrosinase inhibition and antioxidant activity of Glycyrrhiza uralensis and figs aqueous extract[D].Wuhan:Huazhong University of Science and Technology(华中科技大学),2013.
17 Zhang SN,Ma DX,Zhang J.Luo Shen Hua Hua calyxpolyphenol extraction and antioxidant,tyrosinase inhibition study[C].Shanghai:China perfume essence Cosmetics Industry Association,2016.
18 Zhao CM.Tyrosinase inhibitor screening and antioxidant activity of Zanthoxylum bungeanum and tyrosinase inhibitory activity[D].Lanzhou:Lanzhou University of Technology(兰州理工大学),2014.