利用酵母双杂交系统筛选与草莓镶脉病毒移动蛋白P1互作的寄主因子
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摘要
构建感染草莓镶脉病毒(SVBV)森林草莓的酵母c DNA文库,利用酵母双杂交系统,筛选出与SVBV P1蛋白互作的15种寄主因子。生物信息学分析发现,这15种寄主因子参与茉莉酸途径、泛素化、光合作用、抗病抗逆、蛋白修饰、蛋白运输和氧化还原等多种生物过程。另外,这些寄主因子还具有其他分子功能,包括氧化还原酶活性、蛋白二硫化物异构酶活性和金属离子结合活性等。本研究初步探讨了P1与寄主因子的互作机理,为揭示SVBV侵染森林草莓以及SVBV在寄主中扩展的分子机制提供理论依据。
A yeast cDNA library of woodland strawberry infected with Strawberry vein banding virus( SVBV)was constructed,and 15 SVBV P1-interacting host factors were screened through a yeast two-hybrid system.Bioinformatics analysis showed that these genes were involved in various biological processes such as jasmonic acid pathway,ubiquitination,photosynthesis,defense response,protein modification,protein transportation and oxidation-reduction process. In addition,these factors also possessed other molecular functions,including oxidoreductase activity,protein disulfide isomerase activity and metal ion binding activity,etc. This study preliminarily discussed the interaction mechanism between P1 and the host factors,providing a theoretical basis for revealing the molecular mechanism of SVBV infection and transport in woodland strawberry.
A yeast cDNA library of woodland strawberry infected with Strawberry vein banding virus( SVBV)was constructed,and 15 SVBV P1-interacting host factors were screened through a yeast two-hybrid system.Bioinformatics analysis showed that these genes were involved in various biological processes such as jasmonic acid pathway,ubiquitination,photosynthesis,defense response,protein modification,protein transportation and oxidation-reduction process. In addition,these factors also possessed other molecular functions,including oxidoreductase activity,protein disulfide isomerase activity and metal ion binding activity,etc. This study preliminarily discussed the interaction mechanism between P1 and the host factors,providing a theoretical basis for revealing the molecular mechanism of SVBV infection and transport in woodland strawberry.
引文
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[14]Zhao J P,Liu Q,Zhang H L,et al.The rubisco small subunit is involved in tobamovirus movement and Tm-22-mediated extreme resistance[J].Plant Physiology,2013,161(1):374-383.
[15]Georgopoulos C,Welch W J.Role of the major heat shock proteins as molecular chaperone[J].Annual Review of Cell Biology,1993,9:601-634.
[16]Wang G D,Cai G H,Kong F Y,et al.Overexpression of tomato chloroplast-targeted DnaJ protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobacco[J].Plant Physiology and Biochemistry,2014,82(3):95-104.
[17]Hennessy F,Boshoff A,Blatch G L.Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function[J].International Journal of Biochemistry&Cell Biology,2005,37(1):177-191.
[18]Kampinga H H,Craig E A.The HSP70 chaperone machinery:J proteins as drivers of functional specificity[J].Nature Reviews Molecular Cell Biology,2010,11(8):579-592.
[19]Liu J Z,Whitham S A.Overexpression of a soybean nuclear localized type-III DnaJ domain-containing HSP40 reveals its roles in cell death and disease resistance[J].The Plant Journal,2013,74(1):110-121.
[20]Shimizu T,Yoshii A,Sakurai K,et al.Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of Tobacco mosaic virus[J].Archives of Virology,2009,154(6):959-967.
[21]Pearl L H,Prodromou C.Structure and mechanism of the HSP90 molecular chaperone machinery[J].Annual Review of Biochemistry,2006,75:271-294.
[22]Krenz B,Windeisen V,Wege C,et al.A plastidtargeted heat shock cognate 70 kDa protein interacts with the Abutilon mosaic virus movement protein[J].Virology,2010,401(1):6-17.
[23]Allen K D,Staehellin L A.Biochemical characterization of photosystemII antenna polypeptide singrana and stromamembranes of spinach[J].Plant Physiology,1992,100(3):1517-1526.
[24]Jansson S,Pichersky E.A nomencalture for the genes encoding the chlorophyll a/b binding proteins of higher plants[J].Plant Molecular Biology Reporter,1992,10(3):242-253.
[25]Kong L F,Wu J X,Lu L N,et al.Interaction between rice stripe virus disease-specific protein and host PsbP enhances virus symptoms[J].Molecular Plant,2014,7(4):691-708.
[2]Honetslegrova J,Mraz I,Spak J,e t al.Detection and isolation of Strawberry vein banding virus in the Czech Republic[J].Acta Horticulturae,1995,385:29-32.
[3]Xiao M,Zhang Z H.Research advance in Strawberry vein banding virus(in Chinese)[J].Liaoning Agricultural Sciences(辽宁农业科学),2005,(4):36-38.
[4]Morris T J,Mullin R H,Schlegel D E,et al.Isolation of a Caulimovirus from strawberry tissue infected with Strawberry vein banding virus[J].Phytopathology,1980,70(2):156-160.
[5]Frazier N W.Detection of graft-transmissible diseases in strawberry by a modified leaf grafting technique[J].Plant Disease Reporter,1974,58(3):203-207.
[6]Hong J,Li D B,Zhou X P.Classification atlas of plant viruses(in Chinese)[M].Beijing:Scientific Press,(北京:科学出版社):2001,40-42.
[7]Pattanaik S,Dey N,Bhattacharyya S,et al.Isolation of full-length transcript promoter from the Strawberry vein banding virus(SVBV)and expression analysis by protoplasts transient assays and in transgenic plants[J].Plant Science,2004,176(3):427-438.
[8]Petrzik K,Benes V,Mraz I,et al.Strawberry vein banding virus-definitive member of the genus Caulimovirus[J].Virus Genes,1998,16(3):303-305.
[9]Ryabova L A,Pooggin M M,Hohn T.Viral strategies of translation initiation:ribosomal shunt and reinitiation[J].Progress in Nucleic Acid Research&Molecular Biology,2002,72(3):1-39.
[10]Hull R,Sadler J,Longstaff M.The sequence of Carnation etched ring virus DNA:comparison with Cauliflower mosaic virus and retroviruses[J].The EMBOJournal,1986,5(12):3083-3090.
[11]Ji X,Qian D,Wei C H,et al.Movement protein Pns6of Rice dwarf phytoreovirus has both ATPase and RNAbinding activities[J].PLoS One,2011,6(9):e24986.
[12]Shimizu T,Yoshii A,Sakurai K,et al.Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of Tobacco mosaic virus[J].Archives of Virology,2009,154(6):959-967.
[13]Fridborg I,Grainger J,Page A,et al.TIP,A novel host factor linking callose degradation with the cell-tocell movement of Potato virus X[J].Molecular Plant-Microbe Interactions,2003,16(2):132-140.
[14]Zhao J P,Liu Q,Zhang H L,et al.The rubisco small subunit is involved in tobamovirus movement and Tm-22-mediated extreme resistance[J].Plant Physiology,2013,161(1):374-383.
[15]Georgopoulos C,Welch W J.Role of the major heat shock proteins as molecular chaperone[J].Annual Review of Cell Biology,1993,9:601-634.
[16]Wang G D,Cai G H,Kong F Y,et al.Overexpression of tomato chloroplast-targeted DnaJ protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobacco[J].Plant Physiology and Biochemistry,2014,82(3):95-104.
[17]Hennessy F,Boshoff A,Blatch G L.Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function[J].International Journal of Biochemistry&Cell Biology,2005,37(1):177-191.
[18]Kampinga H H,Craig E A.The HSP70 chaperone machinery:J proteins as drivers of functional specificity[J].Nature Reviews Molecular Cell Biology,2010,11(8):579-592.
[19]Liu J Z,Whitham S A.Overexpression of a soybean nuclear localized type-III DnaJ domain-containing HSP40 reveals its roles in cell death and disease resistance[J].The Plant Journal,2013,74(1):110-121.
[20]Shimizu T,Yoshii A,Sakurai K,et al.Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of Tobacco mosaic virus[J].Archives of Virology,2009,154(6):959-967.
[21]Pearl L H,Prodromou C.Structure and mechanism of the HSP90 molecular chaperone machinery[J].Annual Review of Biochemistry,2006,75:271-294.
[22]Krenz B,Windeisen V,Wege C,et al.A plastidtargeted heat shock cognate 70 kDa protein interacts with the Abutilon mosaic virus movement protein[J].Virology,2010,401(1):6-17.
[23]Allen K D,Staehellin L A.Biochemical characterization of photosystemII antenna polypeptide singrana and stromamembranes of spinach[J].Plant Physiology,1992,100(3):1517-1526.
[24]Jansson S,Pichersky E.A nomencalture for the genes encoding the chlorophyll a/b binding proteins of higher plants[J].Plant Molecular Biology Reporter,1992,10(3):242-253.
[25]Kong L F,Wu J X,Lu L N,et al.Interaction between rice stripe virus disease-specific protein and host PsbP enhances virus symptoms[J].Molecular Plant,2014,7(4):691-708.