海洋无脊椎动物抗氧化酶研究进展
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  • 英文篇名:Progress in antioxidant enzymes study of marine invertebrates
  • 作者:李亚男 ; 张海滨
  • 英文作者:LI Ya-nan;ZHANG Hai-bin;Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences;University of Chinese Academy of Sciences;
  • 关键词:无脊椎动物 ; SOD ; GST ; GPX ; 环境 ; 基因表达 ; 功能蛋白
  • 英文关键词:invertebrates;;SOD;;GST;;GPX;;environment;;gene expression;;functional proteins
  • 中文刊名:HUTB
  • 英文刊名:Marine Science Bulletin
  • 机构:中国科学院深海科学与工程研究所;中国科学院大学;
  • 出版日期:2018-06-15
  • 出版单位:海洋通报
  • 年:2018
  • 期:v.37;No.218
  • 基金:海南省重大科技计划项目(ZDKJ2016009);; 中科院知识创新工程领域前沿项目(SIDSSE-2014);; 国家重点研发项目(2017YFC0306604)
  • 语种:中文;
  • 页:HUTB201803002
  • 页数:13
  • CN:03
  • ISSN:12-1076/P
  • 分类号:4-16
摘要
海洋无脊椎动物是重要的经济水产生物和海洋生态系统的主要组成者。抗氧化酶系统在其环境适应性以及非特异性免疫中发挥着重要作用。本文从海洋无脊椎动物主要抗氧化酶类的种类、结构、环境适应性、酶学、基因和蛋白研究等几个方面进行综述并对今后海洋无脊椎动物抗氧化酶的相关研究提出展望。
        Marine invertebrates are one of the main groups of important economic organisms and components in the marine ecosystem. Antioxidant enzymes play an important role in both their environmental adaption and nonspecific immunity. In this paper, several main antioxidant enzymes were reviewed in terms of types, structures, environmental adaptability, enzyme researches, gene and protein researches. A future research on antioxidant enzymes of marine invertebrates was also discussed.
引文
Arhontaki K,Eliopoulos E,Goulielmos G,et al,2002.Functional constraints of the cu,znsuperoxide dismutase in species of the Drosophila melanogaster subgroup and phylogenetic analysis.Journal of Molecular Evolution,55(6):745-756.
    Ayala FJ,1986.On the virtues and pitfalls of the molecular evolutionary clock.Journal of Heredity,77(4):226.
    Bainy ACD,Saito E,Carvalho PSM,et al,1996.Oxidative stress in gill,erythrocytes,liver and kidney of Nile tilapia(Oreochromis niloticus)from a polluted site.Aquatic Toxicology,34(2):151-162.
    Balbi T,Fabbri R,Montagna M,et al,2017.Seasonal variability of different biomarkers in mussels(Mytilus galloprovincialis)farmed at different sites of the gulf of la spezia,ligurian sea,italy.Marine Pollution Bulletin,116(1):1-9.
    Bao Y,Li L Zhang G,2008.The manganese superoxide dismutase gene in bay scallop Argopecten irradians:Cloning,3d modelling and mrna expression.Fish&Shellfish Immunology,25(4):425-432.
    Bao Y,Li L,Wu Q,et al,2009a.Cloning,characterization,and expression analysis of extracellular copper-zinc superoxide dismutase gene from bay scallop Argopecten irradians.Fish&Shellfish Immunology,27(1):17-25.
    Bao Y,Li L,Xu F,et al,2009b.Intracellular copper-zinc superoxide dismutase from bay scallop Argopecten irradians:Its gene structure,mrna expression and recombinant protein.Fish&Shellfish Immunology,27(2):210-220.
    Barondeau DP,Kassmann CJ,Bruns CK,et al,2004.Nickel superoxide dismutase structure and mechanism.Biochemistry,43(25):8038-8047.
    Beck MW,2001.The identification,conservation,and management of estuarine and marine nurseries for fish and invertebrates.Bio Science,51(8):633-641.
    Beyer J,Sandvik M,Hylland K,et al,1996.Contaminant accumulation and biomarker responses in flounder(Platichthys flesus)and atlantic cod(Gadus morhua)exposed by caging to polluted sediments in s覬rfjorden,norway.Aquatic Toxicology,36(1-2):75-98.
    Booth J,Boyland E Sims P,1961.An enzyme from rat liver catalysing conjugations with glutathione.Biochemical Journal,79(3):516-524.
    Brouwer M,Hoexum BT,Grater W,et al,2003.Replacement of a cytosolic copper-zinc superoxide dismutase by a novel cytosolic manganese superoxide dismutase in crustaceans that use copper(haemo-cyanin)for oxygen transport.Biochemical Journal,374(Pt 1):219-228.
    Campa-Cordova AI,Hernandez-Saavedra NY Ascencio F,2002.Superoxide dismutase as modulator of immune function in american white shrimp(Litopenaeus vannamei).Comparative Biochemistry&Physiology Toxicology&Pharmacology Cbp,133(4):557-565.
    Chary P,Hallewell RA Natvig DO,1990.Structure,exon pattern,and chromosome mapping of the gene for cytosolic copper-zinc superoxide dismutase(sod-1)from Neurospora crassa.Journal of Biological Chemistry,265(31):18961-18967.
    Chaturvedi S,2001.Molecular cloning,phylogenetic analysis and threedimensional modeling of cu,zn superoxide dismutase(cnsod1)from three varieties of Cryptococcus neoformans.Gene,268(1-2):41-51.
    Chen M,Yang H,Delaporte M,et al,2007.Immune condition of Chlamys farreri in response to acute temperature challenge.Aquaculture,271(1-4):479-487.
    Ciappellano S,Testolin G Porrini M,1989.Effects of durum wheat dietary selenium on glutathione peroxidase activity and se content in longterm-fed rats.Annals of Nutrition&Metabolism,33(1):22.
    Dallas LJ Jha AN,2015.Applications of biological tools or biomarkers in aquatic biota:A case study of the tamar estuary,south west england.Marine Pollution Bulletin,95(2):618.
    Davies IM,2012.Integrated marine environmental monitoring of chemicals and their effects.Fluid Mechanics&Thermodynamics of Our Environment,59:403-408.
    Dong XQ,Zhang DM,Chen YK,et al,2015.Effects of antimicrobial peptides(amps)on blood biochemical parameters,antioxidase activity,and immune function in the common carp(Cyprinus carpio).Fish&Shellfish Immunology,47(1):429-434.
    Epp O,Ladenstein R,Wendel A,1983.The refined structure of the selenoenzyme glutathione peroxidase at 0.2 nm resolution.European Journal of Biochemistry,133(1):51-69.
    Fattman CL,Schaefer LM Oury TD,2003.Extracellular superoxide dismutase in biology and medicine.Free Radical Biology&Medicine,35(3):236-256.
    Flohe L,Günzler WA Schock HH,1973.Glutathione peroxidase:A selenoenzyme.Febs Letters,32(1):132-134.
    Fukuhara R,Kageyama T,2003.Tissue distribution,molecular cloning,and gene expression of cytosolic glutathione peroxidase in japanese monkey.Zoolog Sci,20(7):861-868.
    Gamble SC,Goldfarb PS,Porte C,et al,1995.Glutathione peroxidase and other antioxidant enzyme function in marine invertebrates(Mytilus edulis,Pecten maximus,Carcinus maenas and Asterias rubens).Marine Environmental Research,39(1-4):191-195.
    Giorgio M,Migliaccio E,Orsini F,et al,2005.Electron transfer between cytochrome c and p66generates reactive oxygen species that trigger mitochondrial apoptosis.Cell,122(2):221-233.
    Gonalves R,Scholze M,Ferreira AM,et al,2008.The joint effect of polycyclic aromatic hydrocarbons on fish behavior.Environmental Research,108(2):205.
    Grimaldi DA,1990.A phylogenetic,revised classification of genera in the Drosophilidae(Diptera).Bulletin of the American Museum of Natural History,197(197):1-139.
    Gül S,Belge-Kuruta拶E,Yildiz E,et al,2004.Pollution correlated modifications of liver antioxidant systems and histopathology of fish(Cyprinidae)living in seyhan dam lake,Turkey.Environment International,30(5):605-609.
    Han J,Lu Y,Zheng H,et al,2016.Differential expression of cuznsod gene under low temperature stress in noble scallop Chlamys nobilis with different carotenoid content.Fish&Shellfish Immunology,54:30-39.
    Holan JR,King CK,Sfiligoj BJ,et al,2017.Toxicity of copper to three common subantarctic marine gastropods.Ecotoxicology&Environmental Safety,136:70-77.
    Horng C Y,Lin H C,Lee W,2010.A reproductive toxicology study of phenanthrene in medaka(Oryzias latipes).Archives of Environmental Contamination&Toxicology,58(1):131-139.
    Khessiba A,Roméo M A觙ssa P,2005.Effects of some environmental parameters on catalase activity measured in the mussel(Mytilus galloprovincialis)exposed to lindane.Environmental Pollution,133(2):275-281.
    Kim EJ,Kim HP,Hah YC,et al,1996.Differential expression of superoxide dismutases containing ni and fe-zn in Streptomyces coelicolor.European Journal of Biochemistry,241(1):178-185.
    Lenartova V,Holovska K,Pedrajas JR,et al,1997.Antioxidant and detoxifying fish enzymes as biomarkers of river pollution.Biomarkers,2(4):247-252.
    Lotufo GR,Fleeger JW,1997.Effects of sediment-associated phenanthrene on survival,development and reproduction of two species of meiobenthic copepods.Marine Ecology Progress,151(1-3):91-102.
    Ludwig ML,Metzger AL,Pattridge KA,et al,1991.Manganese superoxide dismutase from Thermus thermophilus:A structural model refined at 1.8 resolution.Journal of Molecular Biology,219(2):335-358.
    Mccord JM Fridovich I,1969.Superoxide dismutase an enzymic function for erythrocuprein(hemocuprein).Journal of Biological Chemistry,244(22):6049-6055.
    Meng X,Liu M,Wang B,et al,2013.Cadmium bioaccumulation and its effect on antioxidant defense system of japanese scallop Mizuhopecten yessoensis.Journal of Animal&Veterinary Advances,12(1):10-16.
    Mourente G,Diazsalvago E,Bell JG,et al,2002.Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream(Sparus aurata l.)fed dietary oxidised oil:Attenuation by dietary vitamin e.Aquaculture,214(1):343-361.
    Murphy R Decoursey TE,2006.Charge compensation during the phagocyte respiratory burst.Biochimica Et Biophysica Acta,1757(8):996-1011.
    Ni D,Song L,Gao Q,et al,2007.The cdna cloning and mrna expressionof cytoplasmic Cu,Zn superoxide dismutase(SOD)gene in scallop Chlamys farreri.Fish&Shellfish Immunology,23(5):1032-1042.
    Nordberg J,Arnér ES,2001.Reactive oxygen species,antioxidants,and the mammalian thioredoxin system.Free Radical Biology&Medicine,31(11):1287-1312.
    Nozikgrayck E,Suliman HB Piantadosi CA,2005.Extracellular superoxide dismutase.International Journal of Biochemistry&Cell Biology,37(12):2466-2471.
    Oost RVD,Beyer J,Vermeulen NPE,2003.Fish bioaccumulation and biomarkers in environmental risk assessment:A review.Environmental Toxicology&Pharmacology,13(2):57-149.
    Oruc EO,Sevgiler Y,Uner N,2004.Tissue-specific oxidative stress responses in fish exposed to 2,4-d and azinphosmethyl.Comparative Biochemistry&Physiology Part C Toxicology&Pharmacology,137(1):43-51.
    Parker MW,Blake CC,1988.Crystal structure of manganese superoxide dismutase from Bacillus stearothemophilus at 2.4魡resolution.Journal of Molecular Biology,199(4):649-661.
    Parker MW,Blake CC,1988.Iron-and manganese-containing superoxide dismutases can be distinguished by analysis of their primary structures.229(2):377-382.
    Penicaud V,Lacoue-Labarthe T,Bustamante P,2017.Metal bioaccumulation and detoxification processes in cephalopods:A review.Environmental Research,123-133.
    Pérez E,Blasco J,SoléM,2004.Biomarker responses to pollution in two invertebrate species:Scrobicularia plana and Nereis diversicolor from the cádiz bay(sw spain).Marine Environmental Research,58(2-5):275-279.
    Porte C,Sole M,Albaigés J,et al,1991.Responses of mixed-function oxygenase and antioxidase enzyme system of Mytilus sp.to organic pollution.Comparative Biochemistry&Physiology C Comparative Pharmacology&Toxicology,100(1-2):183-186.
    Ren X,Wang Z,Gao B,et al,2017.Toxic responses of swimming crab(Portunus trituberculatus)larvae exposed to environmentally realistic concentrations of oxytetracycline.Chemosphere,173:563-571.
    Richardson J,Thomas KA,Rubin BH,et al,1975.Crystal structure of bovine Cu,Zn superoxide dismutase at 3魡resolution:Chain tracing and metal ligands.Proceedings of the National Academy of Sciences,72(4):1349-1353.
    Rodrigues AP,Lehtonen KK,Guilhermino L,et al,2013.Exposure of carcinus maenas to waterborne fluoranthene:Accumulation and multibiomarker responses.Science of the Total Environment,443(3):454.
    Rotruck JT,Pope AL,Ganther HE,et al,1973.Selenium:Biochemical role as a component of glutathione peroxidase.Science,179(4073):588-590.
    Ryan KC,Guce AI,Johnson OE,et al,2015.Nickel superoxide dismutase:Structural and functional roles of his1 and its h-bonding network.Biochemistry,54(4):1016-1027.
    Schmidt W,Power E,Quinn B,2013.Seasonal variations of biomarker responses in the marine blue mussel(Mytilus spp.).Marine Pollution Bulletin,74(1):50-55.
    Serafim A,Lopes B,Company R,et al,2008.Comparative petroleum hydrocarbons levels and biochemical responses in mussels from hydrothermal vents(Bathymodiolus azoricus)and coastal environments(Mytilus galloprovincialis).Marine Pollution Bulletin,57(6-12):529.
    Shi H,Liao X,Wu L,et al,2004.Tissue culture of cucumber and changes in protein content and activity of antioxidases.Journal of Hebei University,24(6):628-631.
    Stallings WC,Metzger AL,Pattridge KA,et al,1991.Structure-function relationships in iron and manganese superoxide dismutases.Free Radical Research,12-13 Pt 1(1):259-268.
    Stallings WC,Pattridge KA,Strong RK,et al,1985.The structure of manganese superoxide dismutase from Thermus thermophilus HB8 at2.4-A resolution.Journal of Biological Chemistry,260(30):16424-16432.
    Stallings WC,Powers TB,Pattridge KA,et al,1983.Iron superoxide dismutase from escherichia coli at 3.1魡resolution:A structure unlike that of copper-zinc protein at both monomer and dimer levels.Proceedings of the National Academy of Sciences,80(13):3884-3888.
    Tainer JA,Getzoff ED,Beem KM,et al.1982.Determination and analysis of the 2魡structure of copper,zinc superoxide dismutase.Journal of Molecular Biology,160(2):181-217.
    Takebe G,Yarimizu J,Saito Y,et al,2002.A comparative study on the hydroperoxide and thiol specificity of the glutathione peroxidase family and selenoprotein p.Journal of Biological Chemistry,277(43):41254-41258.
    Tao Y,Pan L,Zhang H,et al,2013.Identification of genes differentially expressed in clams Ruditapes philippinarum in response to endosulfan after different exposure time.Ecotoxicology&Environmental Safety,89(11):108.
    Wang H,Yang H,Liu J,et al,2016.Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis.Acta Oceanlogica Sinica,35(4):51-57.
    Winston GW,Giulio RTD,1991.Prooxidant and antioxidant mechanisms in aquatic organisms.Aquatic Toxicology,19(2):137-161.
    Winterbourn CC,1995.Australasian society of clinical and experimental pharmacologists and toxicologists,1994:Free radical toxicology and antioxidant defence.Clinical&Experimental Pharmacology&Physiology,22(11):877-880.
    Xu X,Leng JY,Gao F,et al,2014.Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization.Febs Letters,588(9):1580.
    Yang J,Wei X,Xu J,et al,2012.A sigma-class glutathione s-transferase from Solen grandis that responded to microorganism glycan and organic contaminants.Fish and Shellfish Immunology,32(6):1198-1204.
    Youn HD,Kim EJ,Roe JH,et al,1996.A novel nickel-containing superoxide dismutase from Streptomyces spp.Biochemical Journal,318(3):889-896.
    Zhang K,Zhang Y,Zi J,et al,2017.Production of human Cu,Zn SODwith higher activity and lower toxicity in E.coli via mutation of free cysteine residues.
    Zhang T,Chabory E,Britan A,et al,2008.Gpx5,the selenium-independent glutathione peroxidase-encoding single copy gene is differentially expressed in mouse epididymis.20(5):615-625.
    Zheng L,Wu B,Liu Z,et al,2015.A manganese superoxide dismutase(Mn SOD)from ark shell,Scapharca broughtonii:Molecular characterization,expression and immune activity analysis.Fish&Shellfish Immunology,45(2):656-665.
    Zhang Z,Guo R,Li M,2017.Identification,characterization and expression analysis of superoxide dismutase genes in Dastarcus helophoroides(coleoptera:Bothrideridae).Gene,606:25.
    包永波,2009.海湾扇贝超氧化物歧化酶家族基因结构、表达和多态性分析.中国科学院研究生院(海洋研究所).
    蔡立哲,马丽,高阳,等,2005.蒽、菲、芘混合液对菲律宾蛤仔抗氧化酶活性的影响.海洋科学,29(8):47-52.
    蔡中华,陈艳萍,周进,等,2012.生物标志物(biomarkers)在海洋环境监测中的研究与进展.生命科学,(9):73-86.
    常悦,2013.四角蛤蜊CAT、GPX抗氧化酶基因的克隆.内蒙古农业大学.
    陈鸿鹏,谭晓风,2007.超氧化物歧化酶(SOD)研究综述.经济林研究,25(1):59-65.
    陈淮杨,刘望夷,1996.从超氧化物歧化酶的分布和结构看其分子进化.生物化学与生物物理进展,23(5):408-413.
    陈琳琳,张高生,陈静,等,2011.汞、硒暴露对紫贻贝(Mytilus edulis)抗氧化酶系统的影响.生态毒理学报,06(4):383-388.
    邓思平,赵云涛,朱春华,等,2012.镉对尖紫蛤抗氧化酶活性及脂质过氧化的影响.水生生物学报,36(4):689-695.
    董冉,蒋玫,李磊,等,2016.0#柴油水溶性成分对黑鲷肝脏、鳃、肌肉组织中抗氧化酶活性的影响.海洋渔业,2016,38(2):190-197.
    段立柱,湛垚垚,胡婉彬,等,2014.Cd2+、Cr6+单一胁迫对幼参体壁三种免疫相关酶活力的影响.“全球变化下的海洋与湖沼生态安全”学术交流会论文摘要集.
    方春华,乔琨,刘智禹,等.2016.海洋生物中抗氧化酶的研究进展.福建水产,38(4):331-342.
    管越强,李利,王慧春,等,2010.低氧胁迫对日本沼虾呼吸代谢和抗氧化能力的影响.河北大学学报(自然科学版),30(3):301-306.
    季延滨,于雯雯,孙金辉,等.2008.盐度骤降对南美白对虾仔虾抗氧化机能的影响.天津农学院学报,15(4):19-23.
    蒋凤华,高伟,赵美丽,等,2012.原油污染对栉孔扇贝抗氧化酶活性的影响.海洋科学,36(7):28-33.
    蒋闰兰,2015.菲对中华绒螯蟹(Eriocheir sinensis)毒性效应的研究.华东师范大学.
    孔祥会,王桂忠,艾春香,等,2003.锯缘青蟹不同器官组织中总抗氧化能力和SOD活性的比较研究.应用海洋学学报,22(4):469-474.
    李娟,2010.海湾扇贝抗氧化相关基因的克隆、表达和结构分析.中国科学院研究生院(海洋研究所).
    李明,张思,张建清,等,2016.温度、盐度和p H对仿刺参体腔细胞活性氧产生的影响.水产科学,35(4):346-351.
    李巧梅,王清,于倩,等,2013.As(Ⅲ)暴露对紫贻贝抗氧化酶活性和脂质过氧化的影响.海洋通报,32(4):429-433.
    林群,梁旭方,胡永乐,等,2009.近江牡蛎4种类型gst基因cdna全序列的克隆与分析.生态毒理学报,4(2):237-243.
    刘娜,2012.菲律宾蛤仔(Venerupis philippinarum)在苯并(a)芘胁迫下差异基因的筛选与分子生物标志物的研究.中国海洋大学.
    米盛景,王立俊,王秀利,2011.菲、芘、壬基酚对黑褐新糠虾的急性毒性.河北渔业,(8):5-8.
    倪多娇,2007.栉孔扇贝抗氧化酶基因的克隆与表达分析.中国科学院研究生院(海洋研究所).
    钱佳慧,栗志民,申玉春,等,2015.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究.南方水产科学,11(6):49-57.
    乔新荣,张继英,2016.植物谷胱甘肽过氧化物酶(GPX)研究进展.生物技术通报,32(9):7-13.
    任加云,潘鲁青,苗晶晶,2006.苯并(a)芘和苯并(k)荧蒽混合物对栉孔扇贝毒理学指标的影响.环境科学学报,26(7):1180-1186.
    孙福红,2006.沙蚕(Nereis diversicolor)暴露于镉、铜和石油烃单一与复合污染的生态毒理化学研究.东北农业大学.
    孙虎山,李光友,2000.硒多糖和酵母聚糖对栉孔扇贝血淋巴中二种抗氧化酶活力的影响.中国海洋湖沼药物学术开发研讨会.
    汪飞,黄小平,2012.海洋沉积物中重金属对底栖无脊椎动物的生物有效性.生态学杂志,31(1):207-214.
    王冬群,李太武,苏秀榕,2004.贝类同工酶研究进展.海洋科学,28(11):72-76.
    王冬群,李太武,苏秀榕,2004.三种蛏不同组织同工酶的比较分析.应用海洋学学报,23(2):131-137.
    王静凤,2004.重金属在海产贝类体内的累积及其影响因素的研究.中国海洋大学.
    王秋实,李霞,李丽萍,2007.镉对中间球海胆性腺脂质过氧化的影响.大连海洋大学学报,22(4):270-272.
    王伟伟,2006.不同虾类超氧化物歧化酶性质比较研究.河北大学.
    王振,2010.多齿围沙蚕(Periereis nuntia savigy)对苯并(a)芘的毒性响应.汕头大学.
    吴成龙,2010.皱纹盘鲍抗氧化基因的克隆及其在营养调控下表达的研究.中国海洋大学.
    奚倩,2014.伴随氧化应激的紫外线诱导刺参Stichopus japonicus自溶启动相关因素的研究.大连工业大学.
    谢秋玲,郭勇,1997.超氧化物歧化酶活力测定方法及其进展.今日药学,(4):1-4.
    辛瑞晓,易飞,张蕊,等,2012.哈维氏弧菌铁超氧化物歧化酶基因表达及免疫作用研究.水生生物学报,36(2):212-219.
    杨海灵,聂力嘉,朱圣庚,等,2006.谷胱甘肽硫转移酶结构与功能研究进展.成都大学学报(自然科学版),25(1):19-24.
    杨涛,陈海刚,蔡文贵,等,2011.菲胁迫对红鳍笛鲷急、慢性毒性效应的研究.水产学报,35(2):298-304.
    殷悦,熊德琪,张欣欣,等,2016.消油剂处理180~#燃料油对黄海胆(Glyptocidaris crenularis)抗氧化酶系统影响的初步探究.海洋科学进展,34(2):292-303.
    臧元奇,2012.刺参(Apostichopus japonicus selenka)对典型环境胁迫的生理生态学响应及其机制研究.中国海洋大学.
    张警予,2015.紫外线诱导刺参相关酶的变化及SOD酶学性质的研究.大连工业大学.
    张培玉,唐学玺,董双林,2007.蒽对太平洋牡蛎不同组织抗氧化酶活性差异性影响与膜脂质过氧化研究.海洋环境科学,26(5):434-437.
    张艳红,廖晓全,袁勤生,2001.人锰超氧化物歧化酶(Hmn-SOD)的研究进展.药物生物技术,8(6):352-356.
    赵欢,赵新达,岳宗豪,等,2014.苯并(a)芘对双齿围沙蚕抗氧化酶活性和细胞色素p450基因表达的影响.大连海洋大学学报,(4):342-346.
    郑慧,李彬,荣小军,等,2014.盐度和溶解氧对刺参非特异性免疫酶活性的影响.渔业科学进展,35(1):118-124.
    郑利兵,2015.魁蚶转录组文库的构建分析及免疫相关基因的克隆、表达、抗菌活性研究.上海海洋大学.
    郑萍萍,王春琳,宋微微,等,2010.盐度胁迫对三疣梭子蟹血清非特异性免疫因子的影响.水产科学,29(11):634-638.
    庄树宏,刘雪梅,1998.烟台海域潮间带无脊椎动物对Pb,Cd,Cu,Zn的富集作用.海洋通报,(2):42-50.
    邹慧斌,2005.栉孔扇贝和海湾扇贝g型溶菌酶基因及栉孔扇贝硒结合蛋白基因的克隆和表达研究.中国海洋大学.

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