含腐殖酸还原菌的污泥降解底泥中溶解性有机质
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摘要
在实验室条件下,以天津市某污水处理厂二沉池剩余污泥为活性污泥,探究其在投入河道后对底泥中溶解性有机质(DOM)的降解效果及优化措施。结果表明:使用蒽醌-2,6-双磺酸钠(AQDS)驯化活性污泥30 d,可以将其中的腐殖酸还原菌浓度提高约33倍,同时显著提高对底泥DOM的降解效果,降解率由负值提高至22%;腐殖酸还原菌对DOM的降解率随底泥腐殖化程度升高而升高,底泥的E_3/E_4值为6. 34时,DOM降解率仅为30%,E_3/E_4值为4. 54时,DOM降解率为49%;投加Ca(NO_3)_2可以显著提高DOM降解率,由54%提高至71%,最佳投加量为0. 32 g/L;投加促生剂同样可以显著提高DOM降解率,由52%提高至75%,最佳投加量为0. 04 g/L。
The residual sludge from the secondary sedimentation tank of a sewage treatment plant in Tianjin was used as activated sludge under laboratory conditions,to study its degradation effect on dissolved organic matter( DOM) in sediment and optimization measures after it was put into river. The results showed that: the concentration of humic acid reducing bacteria in activated sludge could be increased by about 33 times after acclimated with anthraquinone-2,6-disulfonic acid sodium( AQDS) for 30 days,and the degradation effect of DOM in sediment could be significantly improved,with the degradation rate increasing to 22% from negative value; the degradation rate of DOM by humic acid reducing bacteria increased with the increase of the degree of sediment colonization; when E_3/E_4 value of sediment was 6. 34,the degradation rate of DOM was only 30%; when E_3/E_4 value was 4. 54,the degradation dosage rate of DOM was 49%; the degradation rate of DOM could be increased significantly through addition of Ca( NO_3)_2,from 54% to 71%,and the optimal addition dosage was 0. 32 g/L; the degradation rate of DOM also could be increased significantly through addition of biostimulant,from 52% to 75%,and the optimal addition dosage was 0. 04 g/L.
The residual sludge from the secondary sedimentation tank of a sewage treatment plant in Tianjin was used as activated sludge under laboratory conditions,to study its degradation effect on dissolved organic matter( DOM) in sediment and optimization measures after it was put into river. The results showed that: the concentration of humic acid reducing bacteria in activated sludge could be increased by about 33 times after acclimated with anthraquinone-2,6-disulfonic acid sodium( AQDS) for 30 days,and the degradation effect of DOM in sediment could be significantly improved,with the degradation rate increasing to 22% from negative value; the degradation rate of DOM by humic acid reducing bacteria increased with the increase of the degree of sediment colonization; when E_3/E_4 value of sediment was 6. 34,the degradation rate of DOM was only 30%; when E_3/E_4 value was 4. 54,the degradation dosage rate of DOM was 49%; the degradation rate of DOM could be increased significantly through addition of Ca( NO_3)_2,from 54% to 71%,and the optimal addition dosage was 0. 32 g/L; the degradation rate of DOM also could be increased significantly through addition of biostimulant,from 52% to 75%,and the optimal addition dosage was 0. 04 g/L.
引文
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[2]唐东民,伍钧,唐勇,等.溶解性有机质对有机污染物环境行为的影响及其环境意义[J].四川环境,2009,28(2):86-90.
[3]高忠霞,周建斌,王祥,等.不同培肥处理对土壤溶解性有机碳含量及特性的影响[J].土壤学报,2010,47(1):115-121.
[4]周江敏,陈华林,代静玉.溶解性有机质在土壤固碳中的意义[J].土壤通报,2011,42(6):1508-1514.
[5] Stevenson F J. Humus chemistry:genesis,composition,reactions[J]. Soil Science,1982,135(2):129-130.
[6]李俊,栾富波,谢丽,等.腐殖酸还原Fe(Ⅲ)的影响因素研究[J].环境污染与防治,2009,31(2):23-26.
[7]贾华丽,郗敏,孔范龙,等.土壤溶解性有机质生物降解研究进展[J].生态科学,2016,35(2):183-188.
[8]范廷玉,严家平,王顺,等.采煤沉陷水域底泥及周边土壤性质差异分析及其环境意义[J].煤炭学报,2014,39(10):2075-2082.
[9] Baham J,Sposito G. Chemistry of water-soluble,metal-complexing ligands extracted from an anaerobically-digested sewage sludge[J].Journal of Environmental Quality,1983,12(1):96-100.
[10] Artinger R, Buckau G, Geyer S, et al. Characterization of groundwater humic substances:influence of sedimentary organic carbon[J]. Applied Geochemistry,2000,15(1):97-116.
[11] Martinez C M,Alvarez L H,Celis L B,et al. Humus-reducing microorganisms and their valuable contribution in environmental processes[J]. Applied Microbiology&Biotechnology,2013,97(24):10293-10308.
[12] Lovley D R, Coates J D, Blunt-harris E L, et al. Humic substances as electron acceptors for microbial respiration[J].Nature,1996,382(6950):445-448.
[13] Block J C,Mathieu L,Servais P,et al. Indigenous bacterial inocula for measuring the biodegradable dissolved organic carbon(BDOC)in waters[J]. Water Research,1992,26(4):481-486.
[14] Qualls R G,Haines B L. Biodegradability of dissolved organic matter in forest throughfall,soil solution,and stream water[J]. Soil Science Society of America Journal,1992,56(2):578-586.
[15] Wang H,Holden J,Zhang Z J,et al. Concentration dynamics and biodegradability of dissolved organic matter in wetland soils subjected to experimental warming[J]. Science of the Total Environment,2014,470-471:907-916.
[16] Li J,Ziegler S,Lane C S,et al. Warming-enhanced preferential microbial mineralization of humified boreal forest soil organic matter:Interpretation of soil profiles along a climate transect using laboratory incubations[J]. Journal of Geophysical Research Biogeosciences,2015,117(G2):502-504.
[17] Li M T,Zhao L P,Zhang J J. Effect of temperature,p H and salt on fluorescent quality of water extractable organic matter in black soil[J]. Journal of Integrative Agriculture,2013,12(7):1251-1257.
[18]黄建军.城市河道底泥营养盐释放及化学修复研究[D].天津:天津大学,2009.
[19]王霖,种云霄,余光伟,等.黑臭底泥硝酸钙原位氧化的温度影响及微生物群落结构全过程分析[J].农业环境科学学报,2015,34(6):1187-1195.
[20]宗栋良,张光明.硝酸钙在底泥修复中的作用机理及应用现状[J].中国农村水利水电,2006(4):52-54.