漆酶降解梗丝中木质素工艺条件的优化
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摘要
采用3因素5水平的正交实验方法研究了不同酶解时间、酶解温度和漆酶用量对梗丝中木质素降解率的影响,同时对漆酶处理前后梗丝的化学成分和填充值进行测量,结果表明:不同酶解时间、酶解温度和漆酶用量极显著影响着木质素的降解率。使用漆酶降解梗丝中木质素的最佳参数是:酶解时间4 h,酶解温度50℃,漆酶用量10.0μL/g。在该参数条件下,梗丝中的木质素降解效果最好,降解率高达47.3%。使用漆酶处理梗丝后,除了木质素之外,梗丝的其它化学成分含量基本不发生变化,填充值也基本没有影响。在最优的参数条件下,使用漆酶能在不降低梗丝原有品质的前提下有效的降解梗丝中的木质素,提高梗丝的品质和可用性。
The experiment uses three factors five levels of orthogonal experimental method to study the different digestion times,enzymolysis temperatures and dosage of laccase influence on cut stem in lignin degradation rate. the change of chemical composition and filling values in cut stem before and after processing also have been measured. The result showed that:(1) Digestion time,enzyme solution temperature,dosage of laccase in cut stem have significantly effected on the degradation of lignin. The best parameter of the enzymatic hydrolysis is time 4 h,enzymolysis temperature 50 ℃,the dosage of laccase 10.0 μL/g. Under this parameter,the lignin degradation effect is best and the degradation rate is as high as 47.3%.(2) The chemical composition of cut stem don't change after the treatment of laccase except lignin and the same situation appeared on the filling valuses. Under the condition of optimal parameters,the use of laccase can effectively degrade lignin,raising the quality and availability of cut stem,and don't lower the quality of cut stem.
The experiment uses three factors five levels of orthogonal experimental method to study the different digestion times,enzymolysis temperatures and dosage of laccase influence on cut stem in lignin degradation rate. the change of chemical composition and filling values in cut stem before and after processing also have been measured. The result showed that:(1) Digestion time,enzyme solution temperature,dosage of laccase in cut stem have significantly effected on the degradation of lignin. The best parameter of the enzymatic hydrolysis is time 4 h,enzymolysis temperature 50 ℃,the dosage of laccase 10.0 μL/g. Under this parameter,the lignin degradation effect is best and the degradation rate is as high as 47.3%.(2) The chemical composition of cut stem don't change after the treatment of laccase except lignin and the same situation appeared on the filling valuses. Under the condition of optimal parameters,the use of laccase can effectively degrade lignin,raising the quality and availability of cut stem,and don't lower the quality of cut stem.
引文
[1]闫克玉,赵铭钦,褚国海,等.烟草原料学[M].北京:科学出版社,2008:432-446.
[2]于建军.卷烟工艺学[M].北京:中国农业出版社,2003:203-216.
[3]QIU Zengchang,WANG Haiyi.The application research status and progress of lignin[J].South West Pulp And Paper,2004(3):29-32.(in Chinese)
[4]WANG Peng,GU Zhengbiao,CHENG Li.Effect of modification by laccase on property of wood material[J].Journal of Food Science and Biotechnology,2012(9):991-995.(in Chinese)
[5]TAO Hong,SHEN Guanglin,ZHAO Mouming,et al.A kaline Treatment of tobacco stem[J].Tobacco Science&Technology,2009(4):37-40.(in Chinese)
[6]ZHOU Yuanqing,ZHOU Liqing,ZHANG Xin,et al.Preliminary study on enhancing the use value of the cut-stem tobacco through the degaradation of lignin by biotechnology[Q].Journal of Yuxi Teachers’College,2006(6):61-63.(in Chinese)
[7]WANG Jing,LI Yongkuan,Li Kun,et al.Optimization of conditions for reducing pectin in waste tobacco stem with pectinase[J].Tobacco Science&Technology,2013(10):59-62.(in Chinese)
[8]CHEN Xing,SHEN Xiaofeng,GONG Xiaowei,et al.Improving tobacco cut stem quality with microbial preparation[J].Acta Tabacaria Sinica,2013,19(3):83-86.(in Chinese)
[9]ZHAO Mingqin,LI Fangfang,LI Xiaoqiang,et al.study on the influence of different biological agent treatment to the content of aroma component of the ferm entation fluecured tobacco[J].Journal of Yunnan Agricultural University,2007,22(4):519-524.(in Chinese)
[10]YU Jianjun,MA Haiyan,YANG Hanwen,et al.Study on using pectinase to degradate pectin in flute-cured tobacco[J].Acta Agriculturae Jiangxi,2009,21(3):136-138.(in Chinese)
[11]ZHANG Hui.New advance of study on ligninolytic enzymes[J].Tianjin Agricultural Sciences,2006,12(3):8-12.(in Chinese)
[12]WANG Na,LI Xian,WANG Dingwei.The screening of lign in and metabo lice of a fungus from tobacco and its application on tobacco technology[J].Journal of Yunnan Agricultural University,2008(23):64-67.(in Chinese)
[13]DUAN Meng,LI Xian,LI Zhengyong,et al.Application of lignin-degrading fungus to paper-making reconstituted tobacco[J].Chinese Tobacco Science,2009,30(3):69-70.(in Chinese)
[2]于建军.卷烟工艺学[M].北京:中国农业出版社,2003:203-216.
[3]QIU Zengchang,WANG Haiyi.The application research status and progress of lignin[J].South West Pulp And Paper,2004(3):29-32.(in Chinese)
[4]WANG Peng,GU Zhengbiao,CHENG Li.Effect of modification by laccase on property of wood material[J].Journal of Food Science and Biotechnology,2012(9):991-995.(in Chinese)
[5]TAO Hong,SHEN Guanglin,ZHAO Mouming,et al.A kaline Treatment of tobacco stem[J].Tobacco Science&Technology,2009(4):37-40.(in Chinese)
[6]ZHOU Yuanqing,ZHOU Liqing,ZHANG Xin,et al.Preliminary study on enhancing the use value of the cut-stem tobacco through the degaradation of lignin by biotechnology[Q].Journal of Yuxi Teachers’College,2006(6):61-63.(in Chinese)
[7]WANG Jing,LI Yongkuan,Li Kun,et al.Optimization of conditions for reducing pectin in waste tobacco stem with pectinase[J].Tobacco Science&Technology,2013(10):59-62.(in Chinese)
[8]CHEN Xing,SHEN Xiaofeng,GONG Xiaowei,et al.Improving tobacco cut stem quality with microbial preparation[J].Acta Tabacaria Sinica,2013,19(3):83-86.(in Chinese)
[9]ZHAO Mingqin,LI Fangfang,LI Xiaoqiang,et al.study on the influence of different biological agent treatment to the content of aroma component of the ferm entation fluecured tobacco[J].Journal of Yunnan Agricultural University,2007,22(4):519-524.(in Chinese)
[10]YU Jianjun,MA Haiyan,YANG Hanwen,et al.Study on using pectinase to degradate pectin in flute-cured tobacco[J].Acta Agriculturae Jiangxi,2009,21(3):136-138.(in Chinese)
[11]ZHANG Hui.New advance of study on ligninolytic enzymes[J].Tianjin Agricultural Sciences,2006,12(3):8-12.(in Chinese)
[12]WANG Na,LI Xian,WANG Dingwei.The screening of lign in and metabo lice of a fungus from tobacco and its application on tobacco technology[J].Journal of Yunnan Agricultural University,2008(23):64-67.(in Chinese)
[13]DUAN Meng,LI Xian,LI Zhengyong,et al.Application of lignin-degrading fungus to paper-making reconstituted tobacco[J].Chinese Tobacco Science,2009,30(3):69-70.(in Chinese)