不同提取方法对朱砂七总大黄素含量的影响
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摘要
目的研究不同提取方法对朱砂七总大黄素含量的影响。方法分别采用乙醇回流提取法、超声提取法、酶辅助提取法和超声-酶辅助提取法对总大黄素进行提取,紫外-可见分光光度法测定其吸光度,以标准曲线法测定含量。结果与结论不同提取方法对朱砂七总大黄素含量影响较大,超声-酶辅助法效率最高。
OBJECTIVE Effect of different extracting method on the content of total flavines in Polygonum cillinervewas investigated. METHODS The total flavines were extracted using ethanol reflux extraction,ultrasound-assisted extraction,enzyme assisted extraction and ultrasonic-assisted enzymatic hydrolysis extraction. The absorbance was measured with UV-visible spectrophotometry. The content of total flavines were calculated according to the standard curve. RESULTS and CONCLUSION Different extracting methods possess different effect on the content of total flavines. The highest extraction yield was found using ultrasonic-assisted enzymatic hydrolysis extraction.
OBJECTIVE Effect of different extracting method on the content of total flavines in Polygonum cillinervewas investigated. METHODS The total flavines were extracted using ethanol reflux extraction,ultrasound-assisted extraction,enzyme assisted extraction and ultrasonic-assisted enzymatic hydrolysis extraction. The absorbance was measured with UV-visible spectrophotometry. The content of total flavines were calculated according to the standard curve. RESULTS and CONCLUSION Different extracting methods possess different effect on the content of total flavines. The highest extraction yield was found using ultrasonic-assisted enzymatic hydrolysis extraction.
引文
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[6]Suriyaporn Nipornram,Worasit Tochampa,PuntarikaRattanatraiwong,Riantong Singanusong.Optimization of low power ultrasound-assisted extraction of phenolic compounds from mandarin(Citrus reticulata Blanco cv.Sainampueng)peel[J].Food Chemistry,2018,241(15):338-345.
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[8]William Tchabo,Yongkun Ma,Felix N.Engmann,Haining Zhang.Ultrasound-assisted enzymatic extraction(UAEE)of phytochemical compounds from mulberry(Morusnigra)must and optimization study using response surface methodology[J].Industrial Crops and Products,2015,63:214-225.
[2]Yanfang Wu,Xinsheng Wang,Pu Liu,et al.Quantitative Determination of Anthraquinones and Resveratrol in Polygonum Cillinerve(Nakai)Ohwi by HPLC-PAD[J].Journal of AOAC International,2017,100(1):25-29.
[3]李煜,陈波,王卫锋.正交实验法优选朱砂七中总大黄素的最佳提取工艺[J].陕西中医,2004,25(2):169-170
[4]吴艳芳,王新胜,张延萍.酶法提取朱砂七中总大黄素的工艺[J].湖北农业科学,2009,48(5):1214-1216.
[5]王晓梅,苏安明,段虹,等.超声波辅助提取朱砂七总蒽醌的工艺研究[J].广州化工,2010,37(211):26-27.
[6]Suriyaporn Nipornram,Worasit Tochampa,PuntarikaRattanatraiwong,Riantong Singanusong.Optimization of low power ultrasound-assisted extraction of phenolic compounds from mandarin(Citrus reticulata Blanco cv.Sainampueng)peel[J].Food Chemistry,2018,241(15):338-345.
[7]Qian Zhang,Ming-ming Zhou,Pei-lin Chen,et al.Optimization of Ultrasonic-Assisted Enzymatic Hydrolysis for the Extraction of Luteolin and Apigenin from Celery[J].Journal of Food Science,2011,76(5):680-685.
[8]William Tchabo,Yongkun Ma,Felix N.Engmann,Haining Zhang.Ultrasound-assisted enzymatic extraction(UAEE)of phytochemical compounds from mulberry(Morusnigra)must and optimization study using response surface methodology[J].Industrial Crops and Products,2015,63:214-225.