高砷煤矿区大金发藓生化指标反映砷氧化应激特征
|
摘要
通过对贵州省兴仁县猫石头水库上游高砷煤矿区酸性矿山废水流域及附近未污染区设置点位并采集优势藓种——大金发藓(Polytrichum commune Hedw.),测定其可溶性蛋白质含量、过氧化氢(H2O2)含量、总抗氧化水平等生化指标,探究高砷煤矿区优势藓种大金发藓在砷氧化应激环境下表现出的生物地球化学特征。结果表明:大金发藓能吸收砷并进入代谢,污染区大金发藓可溶性蛋白质含量均出现降低,在矿洞出水口处最低,仅为0.151 mg·g~(-1),未污染区含量则最高达0.404 mg·g~(-1),并与各点位砷含量呈负相关,地下部分低于地上部分;大金发藓体内活性氧(ROS)出现累积现象,砷的氧化应激在其体内产生作用,污染区大金发藓H_2O_2含量最高达5.93μmol·g~(-1),相比于未污染区苔藓(3.37μmol·g~(-1))明显升高,地下部分低于地上部分,与砷含量呈正相关;污染区大金发藓对砷氧化应激存在一定抵抗性,总抗氧化水平在极高砷含量情况下出现明显降低,A3点位的总抗氧化能力(ferric reducing/antioxidant power,FRAP值)为0.577,距矿洞距离在A3点位之后的点位砷含量较低,FRAP值也基本平稳。
We measured soluble protein concentration,hydrogen peroxide( H_2O_2) concentration,total antioxidant level and other biochemical indices of samples of dominant moss Polytrichum commune Hedw.,which were collected from the acid mine wastewater basin of arsenic-rich coal district and nearby unpolluted area in the upper reaches of Maoshitou Reservoir of Xingren County,Guizhou Province. The biogeochemical characteristics of P. commune in the arsenic-rich coal district under the conditions of arsenic oxidative stress were examined. The results showed that P. commune absorbed arsenic which was involved in metabolism. The soluble protein concentration in P. commune in the polluted area was lower,with a lowest value of 0.151 mg·g~(-1) in the water outlet of mine hole,while the value was up to 0.404 mg·g~(-1) in the unpolluted area. The concentration of soluble protein had negative correlation with arsenic concentration across all sampling locations. The belowground part of P. commune had lower soluble protein concentration than aboveground part. The active oxygen( ROS) in P. commune was accumulated,indicating that the arsenic oxidative stress on P. commune. The H_2O_2 concentration of P. commune in the polluted area was up to 5.93 μmol·g~(-1),being obviously higher than that in the unpolluted area( 3.37μmol·g~(-1)). The belowground part had lower H_2O_2 concentration than the aboveground part. The H_2O_2 concentration had a positive correlation with the arsenic concentration. P. commune in the polluted area was resistant to the arsenic oxidative stress to a certain degree. The total antioxidant level was significantly decreased under extremely high concentration of arsenic. The ferric reducing/antioxidant power( FRAP value) of site A3 was 0.577. The arsenic concentration was relatively low in the sampling locations after site A3,with the FRAP value being generally stable.
We measured soluble protein concentration,hydrogen peroxide( H_2O_2) concentration,total antioxidant level and other biochemical indices of samples of dominant moss Polytrichum commune Hedw.,which were collected from the acid mine wastewater basin of arsenic-rich coal district and nearby unpolluted area in the upper reaches of Maoshitou Reservoir of Xingren County,Guizhou Province. The biogeochemical characteristics of P. commune in the arsenic-rich coal district under the conditions of arsenic oxidative stress were examined. The results showed that P. commune absorbed arsenic which was involved in metabolism. The soluble protein concentration in P. commune in the polluted area was lower,with a lowest value of 0.151 mg·g~(-1) in the water outlet of mine hole,while the value was up to 0.404 mg·g~(-1) in the unpolluted area. The concentration of soluble protein had negative correlation with arsenic concentration across all sampling locations. The belowground part of P. commune had lower soluble protein concentration than aboveground part. The active oxygen( ROS) in P. commune was accumulated,indicating that the arsenic oxidative stress on P. commune. The H_2O_2 concentration of P. commune in the polluted area was up to 5.93 μmol·g~(-1),being obviously higher than that in the unpolluted area( 3.37μmol·g~(-1)). The belowground part had lower H_2O_2 concentration than the aboveground part. The H_2O_2 concentration had a positive correlation with the arsenic concentration. P. commune in the polluted area was resistant to the arsenic oxidative stress to a certain degree. The total antioxidant level was significantly decreased under extremely high concentration of arsenic. The ferric reducing/antioxidant power( FRAP value) of site A3 was 0.577. The arsenic concentration was relatively low in the sampling locations after site A3,with the FRAP value being generally stable.
引文
丁振华,郑宝山,Finkelman RB,et al.2000.黔西南高砷煤的分布及地球化学特征研究.地球化学,29(5):490-494.
段明宇,吴攀,张翅鹏,等.2017.高砷煤矿污染土壤的小麦砷累积研究.麦类作物学报,37(7):985-991.
官庆松,张瑞雪,吴攀,等.2013.酸性高砷煤矿区苔藓植物的适生性及其砷含量特征.生态学杂志,32(12):3325-3329.
官庆松.2013.高砷煤矿区苔藓植物分布及其砷含量研究初探.中国矿物岩石地球化学学会.中国矿物岩石地球化学学会第14届学术年会论文摘要专辑.中国矿物岩石地球化学学会.南京.11.
李合生.2000.植物生理生化实验原理和技术.北京:高等教育出版社.
刘宏,吴攀.2014.高砷煤矿废水灌溉土壤中砷的垂直分布特征.干旱环境监测,28(2):55-59.
娄玉霞.2013.苔藓植物对重金属污染的响应机理和生物指示的研究(博士学位论文).上海:上海师范大学.
乔绪强.2014.铅(Pb2+)胁迫下荇菜和菹草无菌苗的生理生化特性与容忍机制(博士学位论文).南京:南京师范大学.
冉茂良,高环,尹杰,等.2013.氧化应激与DNA损伤.动物营养学报,25(10):2238-2245.
王凤强,娄红祥,季梅.1999.苔纲植物中双联苄类化合物研究进展.国外医药(植物药分册),(6):242-246.
吴攀,裴廷权,冯丽娟,等.2006.贵州兴仁煤矿区土壤表土与沉积物中砷的环境调查研究.地球与环境,(4):31-35.
谢宏,聂爱国.2007.黔西南高砷煤成因研究.煤田地质与勘探,(6):10-14.
张斌,朱江,王朋,等.2018.柠檬香蜂草对铜的耐性及其积累特征研究.西北植物学报,(1):131-139.
张伟倩.2015.白藜芦醇在砷诱导大鼠肝氧化应激中的作用研究(博士学位论文).哈尔滨:东北农业大学.
张显强.2012.贵州石生藓类对石漠化干旱环境的生态适应性研究(博士学位论文).重庆:西南大学.
朱玲,吴攀,袁旭,等.2013.酸性矿山排水对岩溶地下水的影响---基于特征污染物Fe、Mn、As等的对比.贵州大学学报:自然科学版,30(4):128-131.
Benzie IF,Strain J.1996.The ferric reducing ability of plasma(FRAP)as a measure of"antioxidant power":The FRAPassay.Analytical Biochemistry,239:70-76.
Bradford MM.1976.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry,72:248-254.
Gray SF,Scher JM,Speakman JB,et al.2004.Bioactivity guided isolation of antifungal compounds from the liverwort Bazzania trilobata(L.).Phytochemistry,65:2583-2588.
Kulis M,Esteller M.2004.DNA methylation and cancer.Journal of Clinical Oncology,22:4632-4642.
Kanninen K,White AR,Koistinaho J,et al.2011.Targeting glycogen synthase kinase-3βfor therapeutic benefit against oxidative stress in Alzheimer’s disease:Involvement of the Nrf2-ARE Pathway.International Journal of Alzheimer’s Disease,10:1-9.
Kiychin KT,Ahmad S.2003.Oxidative stress as a possible mode of action for arsenic carcinogenesis.Toxicology Letters,137:3-13
Lin YF,Aarts MG.2012.The molecular mechanism of zinc and cadmium stress response in plants.Cellular&Molecular Life Sciences,69:3187-3206.
Mukherjee SP,Choudhuri MA.1983.Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings.Physiologia Plantarum,58:166-170.
Scher JM,Burgess EJ,Lorimerb SD,et al.2002.A cytotoxic sesquiterpene and unprecedented sesquiterpene-bisbibenzyl compounds from the liverwort Schistochila glaucescens.Tetrahedron,58:7875-7882.
Schwartner C,Bors W,Michel C,et al.1995.Effect of marchantins and related compounds on 5-lipoxygenase and cyclooxygenase and their antioxidant properties:A structure activity relationship study.Phytomedicine,2:113-117.
Schwartner C,Michel C,Stettmaier K,et al.1996.Marchantins and related polyphenols from liverwort:Physico-chemical studies of their radical-scavenging properties.Free Radical Biology and Medicine,20:237-244.
Taira Z,Takei M,Endo K,et al.1994.Marchantin A trimethyl ether:Its molecular structure and tubocurarine-like skeletal muscle relaxation activity.Chemical and Pharmaceutical Bulletin,42:52-56.
段明宇,吴攀,张翅鹏,等.2017.高砷煤矿污染土壤的小麦砷累积研究.麦类作物学报,37(7):985-991.
官庆松,张瑞雪,吴攀,等.2013.酸性高砷煤矿区苔藓植物的适生性及其砷含量特征.生态学杂志,32(12):3325-3329.
官庆松.2013.高砷煤矿区苔藓植物分布及其砷含量研究初探.中国矿物岩石地球化学学会.中国矿物岩石地球化学学会第14届学术年会论文摘要专辑.中国矿物岩石地球化学学会.南京.11.
李合生.2000.植物生理生化实验原理和技术.北京:高等教育出版社.
刘宏,吴攀.2014.高砷煤矿废水灌溉土壤中砷的垂直分布特征.干旱环境监测,28(2):55-59.
娄玉霞.2013.苔藓植物对重金属污染的响应机理和生物指示的研究(博士学位论文).上海:上海师范大学.
乔绪强.2014.铅(Pb2+)胁迫下荇菜和菹草无菌苗的生理生化特性与容忍机制(博士学位论文).南京:南京师范大学.
冉茂良,高环,尹杰,等.2013.氧化应激与DNA损伤.动物营养学报,25(10):2238-2245.
王凤强,娄红祥,季梅.1999.苔纲植物中双联苄类化合物研究进展.国外医药(植物药分册),(6):242-246.
吴攀,裴廷权,冯丽娟,等.2006.贵州兴仁煤矿区土壤表土与沉积物中砷的环境调查研究.地球与环境,(4):31-35.
谢宏,聂爱国.2007.黔西南高砷煤成因研究.煤田地质与勘探,(6):10-14.
张斌,朱江,王朋,等.2018.柠檬香蜂草对铜的耐性及其积累特征研究.西北植物学报,(1):131-139.
张伟倩.2015.白藜芦醇在砷诱导大鼠肝氧化应激中的作用研究(博士学位论文).哈尔滨:东北农业大学.
张显强.2012.贵州石生藓类对石漠化干旱环境的生态适应性研究(博士学位论文).重庆:西南大学.
朱玲,吴攀,袁旭,等.2013.酸性矿山排水对岩溶地下水的影响---基于特征污染物Fe、Mn、As等的对比.贵州大学学报:自然科学版,30(4):128-131.
Benzie IF,Strain J.1996.The ferric reducing ability of plasma(FRAP)as a measure of"antioxidant power":The FRAPassay.Analytical Biochemistry,239:70-76.
Bradford MM.1976.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry,72:248-254.
Gray SF,Scher JM,Speakman JB,et al.2004.Bioactivity guided isolation of antifungal compounds from the liverwort Bazzania trilobata(L.).Phytochemistry,65:2583-2588.
Kulis M,Esteller M.2004.DNA methylation and cancer.Journal of Clinical Oncology,22:4632-4642.
Kanninen K,White AR,Koistinaho J,et al.2011.Targeting glycogen synthase kinase-3βfor therapeutic benefit against oxidative stress in Alzheimer’s disease:Involvement of the Nrf2-ARE Pathway.International Journal of Alzheimer’s Disease,10:1-9.
Kiychin KT,Ahmad S.2003.Oxidative stress as a possible mode of action for arsenic carcinogenesis.Toxicology Letters,137:3-13
Lin YF,Aarts MG.2012.The molecular mechanism of zinc and cadmium stress response in plants.Cellular&Molecular Life Sciences,69:3187-3206.
Mukherjee SP,Choudhuri MA.1983.Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings.Physiologia Plantarum,58:166-170.
Scher JM,Burgess EJ,Lorimerb SD,et al.2002.A cytotoxic sesquiterpene and unprecedented sesquiterpene-bisbibenzyl compounds from the liverwort Schistochila glaucescens.Tetrahedron,58:7875-7882.
Schwartner C,Bors W,Michel C,et al.1995.Effect of marchantins and related compounds on 5-lipoxygenase and cyclooxygenase and their antioxidant properties:A structure activity relationship study.Phytomedicine,2:113-117.
Schwartner C,Michel C,Stettmaier K,et al.1996.Marchantins and related polyphenols from liverwort:Physico-chemical studies of their radical-scavenging properties.Free Radical Biology and Medicine,20:237-244.
Taira Z,Takei M,Endo K,et al.1994.Marchantin A trimethyl ether:Its molecular structure and tubocurarine-like skeletal muscle relaxation activity.Chemical and Pharmaceutical Bulletin,42:52-56.