肉鸭消化道酵母益生菌酸胁迫耐受性及其机理研究
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摘要
从肉鸭消化道内分离出的7株酿酒酵母益生菌中,筛选出高耐酸性的菌株,并探究酵母细胞内Na+、K+浓度及H~+-ATPase活性与环境pH的相关性。结果显示:不同酵母菌株酸胁迫耐受性存在差异。酵母菌株通过泵出H~+吸收K~+来维持胞内pH稳定,表明膜H+-ATPase和K+在酵母菌株耐酸中发挥作用。7株酿酒酵母细胞膜H~+-ATPase活性与其生长环境的pH呈负相关。Y-2和Y-7两株酵母菌株相对于其他菌株耐酸性更佳,可作为后续肉鸭专用饲用酵母益生菌的备选菌株。
This experiment aimed to screen low pH resistant strains from 7 Saccharomyces cerevisiaes which separated from the digestive tract of the meat duck.Moreover,the relationship between the Na~+,K~+ concentration,the activity of H~+-ATPase and the pH of yeast cells were explored.The results showed that the ability of acid tolerance of different strains was variance.The S.cerevisiae strains could maintain a neutral cytoplasmic pH by pumping H~+ and absorbing K~+,which proved the function of H~+-ATPase and K~+ in its acidic tolerance.The H~+-ATPase activity showed a negative correlation with the growth pH of 7 S.cerevisiae strains.The tolerance to low pH of test strains Y-2 and Y-7 were better than others,which could be used as a follow-up for alternative special probiotic candidate fodder yeast strains for duck.
This experiment aimed to screen low pH resistant strains from 7 Saccharomyces cerevisiaes which separated from the digestive tract of the meat duck.Moreover,the relationship between the Na~+,K~+ concentration,the activity of H~+-ATPase and the pH of yeast cells were explored.The results showed that the ability of acid tolerance of different strains was variance.The S.cerevisiae strains could maintain a neutral cytoplasmic pH by pumping H~+ and absorbing K~+,which proved the function of H~+-ATPase and K~+ in its acidic tolerance.The H~+-ATPase activity showed a negative correlation with the growth pH of 7 S.cerevisiae strains.The tolerance to low pH of test strains Y-2 and Y-7 were better than others,which could be used as a follow-up for alternative special probiotic candidate fodder yeast strains for duck.
引文
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[3]张国顺,洪青,刘智,等.一株特耐酸酵母R1的分离及其耐酸机理研究[J].微生物学报,2004,44(5):631~635.
[4]张秋华,张敏.酵母菌发酵杂粕生产生物菌体蛋白饲料初探[J].中国饲料,2010,2:32~34.
[5]张霞,王学东,李彪,等.肉鸭消化道酵母益生菌的分离与鉴定[J].饲料工业,2015,22:59~64.
[6]Argyri A A,Zoumpopoulou G,Karatzas K A G,et al.Selection of potential probiotic lactic acid bacteria from fermented olives by invitro,tests[J].Food Microbiology,2013,33(2):282~291.
[7]Arthur H,Watson K.Thermal adaptation in yeast:growth temperatures,membrane lipid,and cytochrome composition of psychrophilic,mesophilic,and thermophilic yeasts[J].Journal of Bacteriology,1976,128(1):56~68.
[8]Cotter P D,Hill C.Surviving the acid test:responses of gram-positive bacteria to low p H[J].Microbiology and Molecular Biology Reviews,2003,67(3):429~453.
[9]Foster J W,Hall H K.Adaptive acidification tolerance response of Salmonella typhimurium.[J].Critical Reviews in Microbiology,1995,21(4):771~778.
[10]Garcíahernández Y,Rodríguez Z,Brandao L R,et al.Identification and in vitro screening of avian yeasts for use as probiotic.[J].Research in Veterinary Science,2012,93(2):798~802.
[11]Ma son A B,Kardos T B,Monk B C.Regulation and p H-dependent expression of a bilaterally truncated yeast plasma membrane H+-ATPase[J].Biochimica et Biophysica Acta(BBA)-Biomembranes,1998,1372(2):261~271.
[12]Patrizia Albertano,Gabriele Pinto,Salvatore Santisi,et al.Spermatozopsis acidophila Kalina(Chlorophyta,Volvocales),a little known alga from highly acidic environments[J].Giornale Botanico Italiano,1981,115(2~3):65~76.
[13]Sekler I,Pick U.Purification and Properties of a Plasma Membrane H+-ATPase from the Extremely Acidophilic Alga Dunaliella acidophila.[J].Plant Physiology,1993,101(101):1055~1061.
[14]Serrano R,Kiellandbrandt M C,Fink G R.Yeast plasma membrane ATPase is essential for growth and has homology with(Na++K+),K+-and Ca2+-ATPases[J].Nature,1986,319(6055):689~693.