不同用途酵母菌株环境适应性差异研究
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摘要
为明确不同的酵母菌株的环境适应性,以便为工业化生产中筛选理想的菌株,本课题主要研究了6种不同用途的酵母菌株在不同环境胁迫条件下的耐受性,初步分析酵母细胞内Na~+、K~+浓度与环境pH的相关性以及其胞内海藻糖积累量与环境胆酸盐浓度的相关性,并对6株酵母菌株的产气能力进行了比较。结果显示,不同用途的酵母菌株的环境耐受性及产气能力存在差异,不同菌株的酸胁迫耐受性与其细胞内Na~+、K~+浓度变化有密切关系,胆酸盐耐受性与其胞内海藻糖积累量密切相关。研究试验菌株中布拉迪酵母、反刍酵母和畜禽酵母3株酵母菌株的酸胁迫耐受性和胆酸盐耐受性较其余3株酵母菌株强,且6株酵母菌中,反刍酵母、畜禽酵母和布拉迪酵母的产气能力均在中等产气能力以下,故此3种酵母菌株适合作为饲用酵母益生菌株使用。
In order to clarify the environmental adaptability of different yeast strains as well as to lay the foundation for screening the ideal strains in industrial production.This study investigated the different environmental stress tolerance of six different yeast strains,including the correlation between Na~+and K~+concentrations and environmental pH in yeast cells as well as the correlation between intracellular trehalose accumulation and environmental biliary acid salt concentration.Whats' s more,the gas production capacity of six yeast strains were compared.The results showed that the environmental tolerance and gas production ability of yeast strains with different uses were different.The acid stress tolerance of different strains were closely related to the changes in intracellular Na~+and K~+concentrations.Cholic acid tolerance and intracellular seaweed the amount of sugar accumulated was closely related.The strains of Brady yeast,ruminant yeast and livestock yeast were tested for acid stress tolerance and cholate tolerance compared with the other three yeast strains.Moreover,as for the gas production abilities of these six yeast strains,ruminant yeast,livestock yeast and Brady yeast were below the moderate level,so these three yeast strains are eligible strains for feed yeast probiotic use.
In order to clarify the environmental adaptability of different yeast strains as well as to lay the foundation for screening the ideal strains in industrial production.This study investigated the different environmental stress tolerance of six different yeast strains,including the correlation between Na~+and K~+concentrations and environmental pH in yeast cells as well as the correlation between intracellular trehalose accumulation and environmental biliary acid salt concentration.Whats' s more,the gas production capacity of six yeast strains were compared.The results showed that the environmental tolerance and gas production ability of yeast strains with different uses were different.The acid stress tolerance of different strains were closely related to the changes in intracellular Na~+and K~+concentrations.Cholic acid tolerance and intracellular seaweed the amount of sugar accumulated was closely related.The strains of Brady yeast,ruminant yeast and livestock yeast were tested for acid stress tolerance and cholate tolerance compared with the other three yeast strains.Moreover,as for the gas production abilities of these six yeast strains,ruminant yeast,livestock yeast and Brady yeast were below the moderate level,so these three yeast strains are eligible strains for feed yeast probiotic use.
引文
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[2]谭斌.饲用高活性干酵母的研发:[硕士学位论文][D].武汉:华中农业大学,2008.
[3]王山宏,王艳杰.瘤胃膨气在临床方面的治疗和预防[J].畜牧兽医科技信息,2006,5:35~36.
[4]张国顺,洪青,刘智,等.一株特耐酸酵母R1的分离及其耐酸机理研究[J].微生物学报,2004,44(5):631~635.
[5] Didari T,Solki S,Mozaffari S,et al.A systematic review of the safety of probiotics[J].Expert Opin Drug Saf,2014,13(2):227~239.
[6] Hatoum R.,Labrie S.and Fliss I.Antimicrobial and probiotic properties of yeasts:From fundamental to novel applications[J].Front.Microbiol,2012,3:421.
[7] Hofmann A,Hagey L.Bileacids:chemistry,pathochemistry,biology,pathobiology,and therapeutics[J].Cellular and Molecular Life Sciences,2008,65(16):2461~2483.
[8] Isreal S,Uri P.Purification and propeties of a plasma membrane H+-ATPase from the extremily acidophilic alga Dunaliella acidophila[J].Plant Physiol,1993,101:1055~1061.