摘要
为了揭示陆地棉生长调控因子(growth-regulating factors, GRF)家族的功能,本研究利用全基因组信息在陆地棉基因组中鉴定了33个GRF蛋白,并对这些家族成员进行了保守结构域分析、蛋白的基本理化性质分析、进化分析、基因的结构分析、染色体定位、保守基序分析和组织表达模式分析。研究表明,陆地棉GRF均含有QLQ和WRC 2个保守结构域,系统进化分为4个亚家族,基因定位在19条染色体上,基因上有2~19个蛋白编码区域,大多数的基因在柱头、子房和20 d的种子中高量表达。本研究弄清了棉花GRF家族的基本信息,为进一步研究棉花GRF家族成员的功能提供了理论指导。
In order to reveal the function of growth-regulating factors(GRF) in Upland cotton, we used wholegenome bioinformatics to identify 33 GRF in Gossypium hirsutum, and analyzed conservative structure domain,the physicochemical properties, phylogenetic evolution, gene structure, chromosome positioning, conservative motif and gene expression pattern in different tissue. The results showed that 33 GRF genes all contained QLQ and WRC two conserved domains, and were divided into four subfamilies. These genes were locationed on 19 chromosomes, contained 2~19 protein coding regions. Many genes expressed higher in stigma, ovule and seed(20 days post anthesis [dpa]) than other tissues. This results clarify the basic information of GRF family in cotton and provide basis information for further research on the function of GRF gene family members in Gossypium hirsutum.
引文
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