摘要
目的:探讨苦参颗粒溶液对兔耳痤疮模型P38MAPK/ERK相关信号通路的影响及其治疗效果。方法:家兔除空白对照组外,其余各组用kligman法建立兔耳痤疮模型,于造模成功后,予模型组、夫西地酸乳膏组、苦参颗粒100、200、400mg/ml组分别涂敷生理盐水和相应药物,每日一次,连续14d。通过HE染色显微镜下判断其治疗效果;免疫组化检测TNF-α、IL-6、IL-8蛋白的表达;Western blot法检测p-p38MAPK、p-ERK、p38MAPK、ERK蛋白表达;ELISA测定法p-p38MAPK、p-ERK蛋白的含量。结果:与空白组比较,模型组TNF-α、IL-6、IL-8 MOD值和p-p38MAPK、p-ERK表达明显升高,p38MAPK、ERK表达明显下降;与模型组相比,给药各组TNF-α、IL-6和IL-8 MOD值和p-p38MAPK、p-ERK表达明显降低,p38MAPK、ERK表达明显增加。结论:苦参颗粒溶液能降低TNF-α、IL-6、IL-8含量,下调p-p38MAPK、p-ERK蛋白表达,上调p38MAPK、ERK蛋白表达,干预ERK、p38MAPK通路,从而干预痤疮的形成,对痤疮有疗效。
Objective:To investigate the effect of Kushen granule solution on p38 MAPK/ERK related signaling pathway in rabbit ear acne model and its therapeutic effect on acne.Methods:Kligman method was used to established rabbit ear acne model, except for the blank control group. After modeling, drugs or saline were daily given to rats in corresponding groups: the model group,the positive control group,and three doses of Kushen granule solution groups(100、200、400 mg/ml), for 14 days constantly. The therapeutic effect was observed by HE staining microscope. The expressions of TNF-α,IL-6 and IL-8 protein was detected by IHC method(immunohistochemistry). Protein expression of p-p38 MAPK,p-ERK,p38 MAPK and ERK was determined by Western blot. Protein expression of p-p38 MAPK and p-ERK was detected by ELISA.Results:Compared with the blank control group, levels of TNF-α,IL-6 and IL-8 in the model control group were increased,the protein expression of p-p38 MAPK and p-ERK was significantly increased, the protein expression of p38 MAPK and ERK was significantly decreased. Compared with the model group,levels of TNF-α,IL-6 and IL-8 protein in all Kushen granule groups were significantly decreased, the protein expression of p-p38 MAPK and p-ERK protein was significantly decreased,the protein expression of p38 MAPK and ERK protein was significantly increased.Conclusion:Kushen granule solution can decrease the expression levels of TNF-α、IL-6、IL-8、p-p38 MAPK and p-ERK,increase the levels of p38 MAPK and ERK,and intervene in the p38 MAPK/ERK signal pathway. It has certain effect on acne by interfering the formation of acne.
引文
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