多指标正交实验法优选当归止痒口服液的提取工艺
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摘要
目的优选当归止痒口服液的提取工艺。方法以芍药苷转移率、阿魏酸转移率和干浸膏得率为指标,采用正交实验法考察料液比、提取时间和提取次数对当归止痒口服液提取工艺的影响,得出当归止痒口服液的最佳提取工艺。采用HPLC法测定芍药苷和阿魏酸的含量。结果最佳提取工艺为:加12倍量水,回流提取3次,每次1h。结论优选的提取工艺稳定,具有良好的重复性和可操作性,可为工业化生产提供科学依据。
Objective To study and optimize the extraction conditions of Dangguizhiyang Oral Liquid.Methods The extraction procedure of Dangguizhiyang Oral Liquid was optimized by orthogonal experiment with paeoniflorin,ferulic acid and dry extract rate as detection index.Orthogonal experiment was used to study the effects of material-liquid ratio,extraction time and extraction times on the extraction process of Dangguizhiyang Oral Liquid,and the optimum extraction process of Dangguizhiyang Oral Liquid was obtained.And the determination of paeoniflorin and ferulic acid was performed by HPLC.Results The optimized extraction process conditions were determined as follows:the herbs were extracted reflux 3 times,each time for 1 hby 12 folds of water.Conclusion This optimized extraction technique of Dangguizhiyang Oral Liquid is stable,repeatable and operable,and could be extended to industrial production.
Objective To study and optimize the extraction conditions of Dangguizhiyang Oral Liquid.Methods The extraction procedure of Dangguizhiyang Oral Liquid was optimized by orthogonal experiment with paeoniflorin,ferulic acid and dry extract rate as detection index.Orthogonal experiment was used to study the effects of material-liquid ratio,extraction time and extraction times on the extraction process of Dangguizhiyang Oral Liquid,and the optimum extraction process of Dangguizhiyang Oral Liquid was obtained.And the determination of paeoniflorin and ferulic acid was performed by HPLC.Results The optimized extraction process conditions were determined as follows:the herbs were extracted reflux 3 times,each time for 1 hby 12 folds of water.Conclusion This optimized extraction technique of Dangguizhiyang Oral Liquid is stable,repeatable and operable,and could be extended to industrial production.
引文
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[18]张静娇,康凯,赵月然,等.RP-HPLC法测定田七痛经胶囊中阿魏酸、藁本内酯、欧当归内酯A[J].药物评价研究,2018,41(9):1657-1660.
[19]梅全喜,梁斌.川芎茶调散(冲剂)的临床新用途[J].中药材,2001,24(12):911-912.
[2]张敬杰.中医治疗老年性皮肤瘙痒症的研究进展分析[J].四川解剖学杂志,2018,26(2):133-134.
[3]龙枚飞,龙雄初,刘小虎,等.老年性皮肤瘙痒症中西医治疗研究进展[J].河北中医,2017,39(6):945-949.
[4]林星游,王炎,翁雪云.当归饮子加减治疗老年皮肤瘙痒症51例[J].中医药学报,2009,37(5):84-85.
[5]陈坤,余晓玲,陈凌云.参威骨痹颗粒的提取工艺研究[J].西北药学杂志,2018,33(3):323-327.
[6]李凡,王卫锋,张军茹,等.养心颗粒的制备工艺优选[J].西北药学杂志,2017,32(4):490-493.
[7]陶松,刘玲,魏筱华,等.多指标正交试验优选菟杞强肾口服液提取工艺[J].江西医药,2018,53(5):481-485.
[8]韩忠耀,周福军,刘芳,等.多指标综合评分法优选柴胡桂枝颗粒提取工艺[J].天津中医药大学学报,2015,34(2):108-111.
[9]陈亚龙,李昭,曹林林,等.正交实验优选益视口服液纯化工艺[J].长春中医药大学学报,2015,31(6):1115-1118.
[10]李娉婷,陈子娇,贾薇,等.中药胶囊剂中芍药苷含量测定方法优化[J].时珍国医国药,2014,25(10):2357-2359.
[11]谢晓梅,余长柱,徐衡,等.赤芍饮片质量标准研究---芍药苷的含量测定[J].中国中药杂志,2004,29(8):759-762.
[12]刘发全,宋亚伟,吴昊,等.HPLC法测定金芩芍注射液中绿原酸、黄芩苷和芍药苷的含量[J].安徽农业科学,2018,46(24):160-161,219.
[13]王红娟,李彦科,程雯,等.HPLC法测定气血和胶囊中芍药苷的含量[J].中国民族民间医药,2018,27(16):15-16,19.
[14]辛振杰,李庆国.HPLC法测定益坤宁酊中梓醇、麦角甾苷、马蹄诺皂苷、氧化芍药苷、芍药苷内酯苷、芍药苷和α-香附酮[J].现代药物与临床,2018,33(8):1875-1878.
[15]王中华.当归中阿魏酸含量测定及质量标准的研究[J].河北中医,2012,34(7):1058-1063.
[16]王欢欢,黄晓舞.HPLC法测定肾乐胶囊中阿魏酸含量[J].中国药物应用与监测,2018,15(4):204-206.
[17]叶晓娅,薛晓会.HPLC法同时测定当归片中阿魏酸、洋川芎内酯Ⅰ和藁本内酯的含量[J].中国药品标准,2018,19(4):295-298.
[18]张静娇,康凯,赵月然,等.RP-HPLC法测定田七痛经胶囊中阿魏酸、藁本内酯、欧当归内酯A[J].药物评价研究,2018,41(9):1657-1660.
[19]梅全喜,梁斌.川芎茶调散(冲剂)的临床新用途[J].中药材,2001,24(12):911-912.