不同碳源对Delftia tsuruhatensis HT01脱氮性能的影响
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摘要
以一株异养氨氧化菌Delftia tsuruhatensis HT01为研究对象,比较了以十二烷基硫酸钠(SDS)、甘蔗糖蜜、丁二酸钠、乙酸钠、蔗糖、葡萄糖、果糖或柠檬酸钠等为唯一碳源时的生长情况及对TOC、NH_4~+-N、TN的去除率,并通过两轮中试测试了该菌对皮革污水的处理效果.结果表明:HT01在异养条件下能够生成NO_2~--N,并可以在利用SDS(去除率为34%)的同时去除NH_4~+-N和TN(去除率分别为74%和14%);丁二酸钠和乙酸钠分别有利于实现最快的生长速度和最高的TOC去除率(71%),而果糖则有利于实现最高的NH_4~+-N和TN去除率(分别为98%和29%).HT01能够在皮革污水中生长,第2轮中试对COD,NH_4~+-N和TN的去除率分别达到38%,49%和22%.
The growth and the removal ef?ciency of TOC, NH_4~+-N and TN of a heterotrophic ammonia oxidizer Delftia tsuruhatensis HT01 were studied when SDS, cane molasses, sodium succinate, sodium acetate, sucrose, glucose, fructose or sodium citrate was used as sole carbon source. Furthermore, two rounds of the pilot-scale test were performed using leather sewage. The results indicated that NO_2~--N was produced in heterotrophic condition by HT01 and NH_4~+-N, TN and SDS were removed simultaneously at the removal ef?ciency of 74%, 14% and 34%, respectively. Moreover, the highest growth rate and the highest removal ef?ciency of TOC(71%) were observed when sodium succinate and sodium was used as sole carbon source, respectively.While the highest removal ef?ciency of NH_4~+-N(98%) and TN(29%) were observed when fructose was used as sole carbon source.The growth of HT01 was observed in leather sewage, and the removal efficiency of COD, NH_4~+-N and TN was 38%, 49% and 22%,respectively, in the second round of pilot-scale test.
The growth and the removal ef?ciency of TOC, NH_4~+-N and TN of a heterotrophic ammonia oxidizer Delftia tsuruhatensis HT01 were studied when SDS, cane molasses, sodium succinate, sodium acetate, sucrose, glucose, fructose or sodium citrate was used as sole carbon source. Furthermore, two rounds of the pilot-scale test were performed using leather sewage. The results indicated that NO_2~--N was produced in heterotrophic condition by HT01 and NH_4~+-N, TN and SDS were removed simultaneously at the removal ef?ciency of 74%, 14% and 34%, respectively. Moreover, the highest growth rate and the highest removal ef?ciency of TOC(71%) were observed when sodium succinate and sodium was used as sole carbon source, respectively.While the highest removal ef?ciency of NH_4~+-N(98%) and TN(29%) were observed when fructose was used as sole carbon source.The growth of HT01 was observed in leather sewage, and the removal efficiency of COD, NH_4~+-N and TN was 38%, 49% and 22%,respectively, in the second round of pilot-scale test.
引文
[1]叶剑锋.废水生物脱氮处理新技术[M].北京:化学工业出版社,2006:10-12.Ye J F.New technology for biological denitrification of wastewater[M].Beijing:Chemical Industry Press:2006:10-12.
[2]乔森,刘雪洁,周集体.异养硝化-好氧反硝化在生物脱氮方面的研究进展[J].安全与环境学报,2014,14(2):128-135.Qiao S,Liu X J,Zhou J T.Research progress of heterotrophic nitrification-aerobic denitrification in biological denitrification[J].Journal of Safety and Environment,2014,14(2):128-135.
[3]林燕,孔海南,王茸影,等.异养硝化作用的主要特点及其研究动向[J].环境科学,2008,29(11):3291-3296.Lin Y,Kong H N,Wang R Y,et al.Characteristic and prospects of heterotrophic nitrification[J].Environmental science,2008,29(11):3291-3296.
[4]苟莎,黄钧.异养硝化细菌脱氮特性及研究进展[J].微生物学通报,2009,36(2):255-260.Gou S,Huang J.Advances in denitrification characteristics of heterotrophic nitrification bacteria[J].Microbiology,2009,36(2):255-260.
[5]Kim J K,Park K J,Cho K S,et al.Aerobic nitrification-denitrification by heterotrophic Bacillus strains[J].Bioresource Technology,2005,96(17):1897-1906.
[6]邹艳艳,张宇,李明智,等.一株异养硝化-好氧反硝化细菌的分离鉴定及脱氮活性研究[J].中国环境科学,2016,36(3):887-893.Zou Y Y,Zhang Y,Li M Z,et al.Isolation and identification of a heterotrophic nitrification-aerobic denitrification bacterium and its denitrification ability[J].China Environmental Science,2016,36(3):887-893.
[7]王骁静,于德爽,李津,等.海洋异养硝化-好氧反硝化菌y6同步脱氮除碳特性[J].中国环境科学,2017,37(2):686-695.Wang X J,Yu D S,LI J,et al.Separation identification and the characteristics research of simultaneous removal of nitrogen and carbon about Marine heterotrophic nitrification and aerobic denitrification strain y6[J].China Environmental Science,2017,37(2):686-695.
[8]Duan J M,Fang H D,Su B,et al.Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater[J].Bioresource Technology,2015,179(3):421-428.
[9]张树松,樊月婷,孙兴滨,等.菌株Diaphorobacter polyhydroxybutyrativorans SL-205的反硝化特性[J].中国环境科学,2017,37(9):3532-3539.Zhang S S,Fan Y T,Sun X B,et al.Denitrification performance of the bacterium Diaphorobacter polyhydroxybutyrativorans SL-205[J].China Environmental Science,2017,37(9):3532-3539.
[10]Lei Y,Wang Y Q,Liu H J,et al.A novel heterotrophic nitrifying and aerobic denitrifying bacterium,Zobellella Taiwanensis DN-7,can remove high-strength ammonium[J].Applied Microbiology Biotechnology,2016,100(9):4219-4229.
[11]Yang L,Ren Y X,Zhao S Q,et al.Isolation and characterization of three heterotrophic nitrifying-aerobic denitrifying bacteria from a sequencing batch reactor[J].Annals of Microbiology,2016,66(2):737-747.
[12]Chen Q,Ni J R.Ammonium removal by Agrobacterium sp.LAD9capable of heterotrophic nitrification-aerobic denitrification[J].Journal of Bioscience and Bioengineering,2012,113(5):619-623.
[13]Paulo A M S,Plugge C M,García-encina P A,et al.Anaerobic degradation of sodium dodecyl sulfate(SDS)by denitrifying bacteria[J].International Biodeterioration and Biodegradation,2013,84(5):14-20.
[14]Yilmaz F,Icgen B.Characterization of SDS-degrading Delftia acidovorans and in situmonitoring of its temporal succession in SDS-contaminated surface waters[J].Environmental Science&Pollution Research International,2014,21(12):7413-7424.
[15]郑琰晶,陈琳,陈燕平,等.十二烷基硫酸钠对水生生物的急性毒性影响[J].农业环境科学学报,2006,25(增刊):496-498.Zheng Y J,Chen L,Chen Y P,et al.Acute toxicity of sodium dodecyl sulfate(SDS)on selected aquatic organisms[J].Journal of AgroEnvionment Science,2006,25(Supplements):496-498.
[16]Furmanczyk E M,Kaminski M A,Spolnik G,et al.Isolation and characterization of Pseudomonas spp.strains that efficiently decompose sodium dodecyl sulfate[J].Frontiers in Microbiology,2017,8:1-16.
[17]John E M,Rebello S,Asok A K,et al.Pseudomonas plecoglossicida S5,a novel nonpathogenic isolate for sodium dodecyl sulfate degradation[J].Environmental Chemistry Letters,2015,13(1):117-123.
[18]Rahman M F,Rusnam M,Gusmanizar N,et al.Molybdate-reducing and SDS-degrading Enterobacter sp.strain Neni-13[J].Nova Biotechnologica et Chimica,2016,15(2):166-181.
[19]Masdor N.Isolation and characterization of a molybdenum-reducing and SDS-degrading Klebsiella oxytoca strain Aft-7and its bioremediation application in the environment[J].Biodiversitas,2015,16(2):238-246.
[20]关向杰,杨海君,黄水娥,等.一株耐铬不动杆菌对十二烷基硫酸钠的降解特性[J].环境工程学报,2014,8(5):2117-2123.Guan X J,Yang H J,Huang S E,et al.Degradation characteristics of sodium dodecyl sulfate by a chromium resistant Acinetobater Strain[J].Chinese Journal of Environmental Engineering,2014,8(5):2117-2123.
[21]颜丙花,杨海君,罗琳,等.十二烷基硫酸钠降解菌的分离鉴定及其降解能力[J].化工环保,2011,31(2):110-113.Yan B H,Yang H J,Luo L,et al.Isolation,identification of SDS-degrading strain and its degrading capability[J].Environmental Protection of Chemical Industry,2011,31(2):110-113.
[22]刘晶晶,汪苹.一株好氧反硝化菌的反硝化性能研究[J].北京工商大学学报(自然科学版),2008,26(4):5-8.Liu J J,Wang P.Study on denitrification characteristic of an aerobic denitrifier[J].Journal of Beijing Technology and Business University(Natural Science Edition),2008,26(4):8-11.
[23]叶杰旭,林彤晖,骆煜昊,等.1株氯苯高效降解菌的分离鉴定及降解特性[J].环境科学,2017,38(2):802-808.Ye J X,Lin T H,Luo Y H,et al.Isolation and identification of a chlorobenzene-degrading bacterium and its degradation characteristic[J].Environmental Science,2017,38(2):802-808.
[24]金德才,吴学玲,梁任星,等.一株DMP降解菌的分离鉴定及其降解特性[J].微生物学通报,2009,36(9):1318-1323.Jin D C,Wu X L,Liang R X,et al.Isolation,identification and degradation characteristics of a DMP-degrading strain[J].Microbiology,2009,36(9):1318-1323.
[25]王欢,汪苹,张海波.一株戴尔福特菌的异养硝化与好氧反硝化性能研究[J].北京工商大学学报(自然科学版),2008,26(2):1-5.Wang H,Wang P,Zhang H B.Characteristics of heterotrophic nitrification and aerobic denitrification by Delftia tsuruhatensis[J].Journal of Beijing Technology and Business University(Natural Science Edition),2008,26(2):1-5.
[26]王娟,汪苹,项慕飞,等.2株好氧反硝化菌还原能力测定及菌种鉴定[J].环境与健康杂志,2008,25(6):525-529.Wang J,Wang P,Xiang M F,et al.Study on reducing capacity and identification of aerobic denitrifiers[J].Journal of Environment and Health,2008,25(6):525-529.
[27]王欢,汪苹,刘晶晶.好氧反硝化菌的异养硝化性能研究[J].环境科学与技术,2008,31(11):45-47.Wang H,Wang P,Liu J J.Characteristics of heterotrophic nitrification by aerobic denitrifiers[J].Environmental Science&Technology,2008,31(11):45-47.
[28]Li P,Liu D L,Liberat N,et al.High nitrogen removal from wastewater with several new aerobic bacteria isolated from diverse ecosys[J].Journal of Environmental Sciences,2006,18(3):525-529.
[29]侯颖,王飞,李静,等.Delftia sp.T3-6菌株及其粗酶液对2',6'-甲乙基-2-氯乙酰苯胺的降解[J].环境科学学报,2014,34(6):1396-1402.Hou Y,Wang F,Li J Q,et al.Degradation characteristics of2',6'-methylethyl-2-chloroacetanilide by strain Delftia sp.T3-6 and its crude enzyme[J].Acta Scientiae Circumstantiae,2014,34(6):6,1396-1402.
[30]陈香琪,李科,张利平.异氧硝化-好氧反硝化菌株的筛选及初步鉴定[J].河南农业科学,2014,43(3):59-64.Chen X Q,Li K,Zhang L P.Screening and identification of simultaneous nitrification and denitrification bacteria[J].Journal of Henan Agricultural Sciences,2014,43(3):59-64.
[31]匡燕,梁运祥.一株水体高效氨化菌的分离及特性研究[J].环境科学与技术,2013,36(2):19-22.Kuang Y,Liang Y X.Isolation of an efficient ammonifying bacterium and study of its transformation properties[J].Environmental Science&Technology,2013,36(2):19-22.
[32]Joo H S,Hirai M,Shoda M.Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis no.4[J].Journal of Bioscience&Bioengineering.2005,100(2):184-191.
[33]Jin C P,Cheng K.Delftia tsuruhatensis strain KHN-HT01 16Sribosomal RNA gene,partial sequence,GenBank:MG543680.1[EB/OL].https://www.ncbi.nlm.nih.gov/nuccore/MG543680.1/,2018-12-01/2018-12-16.
[34]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002:258-284.State Environmental Protection Administration.Monitoring Method of Water and Waste Water[M],4th ed.Beijing:Chinese Environmental Science Press,2002:258-284.
[35]Jin C P.Unverified:Delftia tsuruhatensis SDS degradation transcriptional activation protein-like gene,partial sequence,GenBank:MH393620.1[EB/OL].https://www.ncbi.nlm.nih.gov/nuccore/MH393620,2018-12-04/2018-12-16.
[36]张若木,李泽琴,陈杨武,等.十二烷基硫酸钠微生物降解研究进展[J].再生资源与循化经济,2016,9(1):34-40.Zhang R M,Li Z Q,Chen Y W,et al.Research advances on microbial degradation of sodium dodecyl sulfate[J].Recyclable Resources and Cyclecar Economy,2016,9(1):34-40.
[37]崔文娟,董琳茜,孙珮石,等.硝化菌的分离及特性分析[J].环境工程学报,2012,6(2):683-686.Cui W J,Dong Li Q,Sun P S,et al.Isolation and characterization of nitrifiers[J].Chinese Journal of Environmental Engineering,2012,6(2):683-686.
[38]何厚波,熊杨,周敬超.生活垃圾填埋场渗滤液的特点及处理技术[J].环境卫生工程,2002,10(4):159-163.He H B,Xiong Y,Zhou J C.The characteristic and treatment technology of municipal solid waste landfill leachate[J].Environmental Sanitation Engineering,2002,10(4):159-163.
[39]董春松,樊耀波,李刚,等.我国垃圾渗滤液的特点和处理技术探讨[J].中国给水排水,2005,21(12):27-31.Dong C S,Fan Y B,Li G,et al.Characteristics of domestic refuse landfill leachate and discussion on treatment technology[J].China Water&Wastewater,2005,21(12):27-31.
[40]陈雷,贺磊,吴立群,等.垃圾渗滤液的处理现状及发展方向[J].环境工程,2016,34(增刊):295-298.Chen L,He L,Wu L Q,et al.Status of landfill leachate treatment and its development direction[J].Environmental Engineering,2016,34(Supplements):295-298.
[41]张培玉,曲洋,于德爽,等.菌株qy37的异养硝化/好氧反硝化机制比较及氨氮加速降解特性研究[J].环境科学,2010,31(8):1819-1826.Zhang P Y,Qu Y,Yu D S,et al.Comparison of heterotrophic nitrification and aerobic denitrification system by strain qy37 and its accelerating removal characteristic of NH4+-N[J].Environmental Science,2010,31(8):1819-1826.
[42]王欣,黄瑞敏,陈克复.制革废水氨氮处理的研究进展[J].皮革化工,2004,21(2):9-13.Wang X,Huang R M,Chen K F.Advances in ammonium removal of tannery wastewater[J].Leather Chemicals,2004,21(2):9-13.
[43]冯景伟,孙亚兵,郑正,等.制革废水处理技术研究进展[J].环境科学与技术,2008,31(6):73-77.Feng J W,Sun Y B,Zheng Z,et al.Recent advances of technology for treatment of tannery wastewater[J].Environmental Science&Technology,2008,31(6):73-77.
[44]游伟民.皮革废水治理技术的研究进展[J].皮革与化工,2009,26(2):16-20.You W M.Research progress in techniques of tannery wastewater treatment[J].Leather and Chemicals,2009,26(2):16-20.
[2]乔森,刘雪洁,周集体.异养硝化-好氧反硝化在生物脱氮方面的研究进展[J].安全与环境学报,2014,14(2):128-135.Qiao S,Liu X J,Zhou J T.Research progress of heterotrophic nitrification-aerobic denitrification in biological denitrification[J].Journal of Safety and Environment,2014,14(2):128-135.
[3]林燕,孔海南,王茸影,等.异养硝化作用的主要特点及其研究动向[J].环境科学,2008,29(11):3291-3296.Lin Y,Kong H N,Wang R Y,et al.Characteristic and prospects of heterotrophic nitrification[J].Environmental science,2008,29(11):3291-3296.
[4]苟莎,黄钧.异养硝化细菌脱氮特性及研究进展[J].微生物学通报,2009,36(2):255-260.Gou S,Huang J.Advances in denitrification characteristics of heterotrophic nitrification bacteria[J].Microbiology,2009,36(2):255-260.
[5]Kim J K,Park K J,Cho K S,et al.Aerobic nitrification-denitrification by heterotrophic Bacillus strains[J].Bioresource Technology,2005,96(17):1897-1906.
[6]邹艳艳,张宇,李明智,等.一株异养硝化-好氧反硝化细菌的分离鉴定及脱氮活性研究[J].中国环境科学,2016,36(3):887-893.Zou Y Y,Zhang Y,Li M Z,et al.Isolation and identification of a heterotrophic nitrification-aerobic denitrification bacterium and its denitrification ability[J].China Environmental Science,2016,36(3):887-893.
[7]王骁静,于德爽,李津,等.海洋异养硝化-好氧反硝化菌y6同步脱氮除碳特性[J].中国环境科学,2017,37(2):686-695.Wang X J,Yu D S,LI J,et al.Separation identification and the characteristics research of simultaneous removal of nitrogen and carbon about Marine heterotrophic nitrification and aerobic denitrification strain y6[J].China Environmental Science,2017,37(2):686-695.
[8]Duan J M,Fang H D,Su B,et al.Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater[J].Bioresource Technology,2015,179(3):421-428.
[9]张树松,樊月婷,孙兴滨,等.菌株Diaphorobacter polyhydroxybutyrativorans SL-205的反硝化特性[J].中国环境科学,2017,37(9):3532-3539.Zhang S S,Fan Y T,Sun X B,et al.Denitrification performance of the bacterium Diaphorobacter polyhydroxybutyrativorans SL-205[J].China Environmental Science,2017,37(9):3532-3539.
[10]Lei Y,Wang Y Q,Liu H J,et al.A novel heterotrophic nitrifying and aerobic denitrifying bacterium,Zobellella Taiwanensis DN-7,can remove high-strength ammonium[J].Applied Microbiology Biotechnology,2016,100(9):4219-4229.
[11]Yang L,Ren Y X,Zhao S Q,et al.Isolation and characterization of three heterotrophic nitrifying-aerobic denitrifying bacteria from a sequencing batch reactor[J].Annals of Microbiology,2016,66(2):737-747.
[12]Chen Q,Ni J R.Ammonium removal by Agrobacterium sp.LAD9capable of heterotrophic nitrification-aerobic denitrification[J].Journal of Bioscience and Bioengineering,2012,113(5):619-623.
[13]Paulo A M S,Plugge C M,García-encina P A,et al.Anaerobic degradation of sodium dodecyl sulfate(SDS)by denitrifying bacteria[J].International Biodeterioration and Biodegradation,2013,84(5):14-20.
[14]Yilmaz F,Icgen B.Characterization of SDS-degrading Delftia acidovorans and in situmonitoring of its temporal succession in SDS-contaminated surface waters[J].Environmental Science&Pollution Research International,2014,21(12):7413-7424.
[15]郑琰晶,陈琳,陈燕平,等.十二烷基硫酸钠对水生生物的急性毒性影响[J].农业环境科学学报,2006,25(增刊):496-498.Zheng Y J,Chen L,Chen Y P,et al.Acute toxicity of sodium dodecyl sulfate(SDS)on selected aquatic organisms[J].Journal of AgroEnvionment Science,2006,25(Supplements):496-498.
[16]Furmanczyk E M,Kaminski M A,Spolnik G,et al.Isolation and characterization of Pseudomonas spp.strains that efficiently decompose sodium dodecyl sulfate[J].Frontiers in Microbiology,2017,8:1-16.
[17]John E M,Rebello S,Asok A K,et al.Pseudomonas plecoglossicida S5,a novel nonpathogenic isolate for sodium dodecyl sulfate degradation[J].Environmental Chemistry Letters,2015,13(1):117-123.
[18]Rahman M F,Rusnam M,Gusmanizar N,et al.Molybdate-reducing and SDS-degrading Enterobacter sp.strain Neni-13[J].Nova Biotechnologica et Chimica,2016,15(2):166-181.
[19]Masdor N.Isolation and characterization of a molybdenum-reducing and SDS-degrading Klebsiella oxytoca strain Aft-7and its bioremediation application in the environment[J].Biodiversitas,2015,16(2):238-246.
[20]关向杰,杨海君,黄水娥,等.一株耐铬不动杆菌对十二烷基硫酸钠的降解特性[J].环境工程学报,2014,8(5):2117-2123.Guan X J,Yang H J,Huang S E,et al.Degradation characteristics of sodium dodecyl sulfate by a chromium resistant Acinetobater Strain[J].Chinese Journal of Environmental Engineering,2014,8(5):2117-2123.
[21]颜丙花,杨海君,罗琳,等.十二烷基硫酸钠降解菌的分离鉴定及其降解能力[J].化工环保,2011,31(2):110-113.Yan B H,Yang H J,Luo L,et al.Isolation,identification of SDS-degrading strain and its degrading capability[J].Environmental Protection of Chemical Industry,2011,31(2):110-113.
[22]刘晶晶,汪苹.一株好氧反硝化菌的反硝化性能研究[J].北京工商大学学报(自然科学版),2008,26(4):5-8.Liu J J,Wang P.Study on denitrification characteristic of an aerobic denitrifier[J].Journal of Beijing Technology and Business University(Natural Science Edition),2008,26(4):8-11.
[23]叶杰旭,林彤晖,骆煜昊,等.1株氯苯高效降解菌的分离鉴定及降解特性[J].环境科学,2017,38(2):802-808.Ye J X,Lin T H,Luo Y H,et al.Isolation and identification of a chlorobenzene-degrading bacterium and its degradation characteristic[J].Environmental Science,2017,38(2):802-808.
[24]金德才,吴学玲,梁任星,等.一株DMP降解菌的分离鉴定及其降解特性[J].微生物学通报,2009,36(9):1318-1323.Jin D C,Wu X L,Liang R X,et al.Isolation,identification and degradation characteristics of a DMP-degrading strain[J].Microbiology,2009,36(9):1318-1323.
[25]王欢,汪苹,张海波.一株戴尔福特菌的异养硝化与好氧反硝化性能研究[J].北京工商大学学报(自然科学版),2008,26(2):1-5.Wang H,Wang P,Zhang H B.Characteristics of heterotrophic nitrification and aerobic denitrification by Delftia tsuruhatensis[J].Journal of Beijing Technology and Business University(Natural Science Edition),2008,26(2):1-5.
[26]王娟,汪苹,项慕飞,等.2株好氧反硝化菌还原能力测定及菌种鉴定[J].环境与健康杂志,2008,25(6):525-529.Wang J,Wang P,Xiang M F,et al.Study on reducing capacity and identification of aerobic denitrifiers[J].Journal of Environment and Health,2008,25(6):525-529.
[27]王欢,汪苹,刘晶晶.好氧反硝化菌的异养硝化性能研究[J].环境科学与技术,2008,31(11):45-47.Wang H,Wang P,Liu J J.Characteristics of heterotrophic nitrification by aerobic denitrifiers[J].Environmental Science&Technology,2008,31(11):45-47.
[28]Li P,Liu D L,Liberat N,et al.High nitrogen removal from wastewater with several new aerobic bacteria isolated from diverse ecosys[J].Journal of Environmental Sciences,2006,18(3):525-529.
[29]侯颖,王飞,李静,等.Delftia sp.T3-6菌株及其粗酶液对2',6'-甲乙基-2-氯乙酰苯胺的降解[J].环境科学学报,2014,34(6):1396-1402.Hou Y,Wang F,Li J Q,et al.Degradation characteristics of2',6'-methylethyl-2-chloroacetanilide by strain Delftia sp.T3-6 and its crude enzyme[J].Acta Scientiae Circumstantiae,2014,34(6):6,1396-1402.
[30]陈香琪,李科,张利平.异氧硝化-好氧反硝化菌株的筛选及初步鉴定[J].河南农业科学,2014,43(3):59-64.Chen X Q,Li K,Zhang L P.Screening and identification of simultaneous nitrification and denitrification bacteria[J].Journal of Henan Agricultural Sciences,2014,43(3):59-64.
[31]匡燕,梁运祥.一株水体高效氨化菌的分离及特性研究[J].环境科学与技术,2013,36(2):19-22.Kuang Y,Liang Y X.Isolation of an efficient ammonifying bacterium and study of its transformation properties[J].Environmental Science&Technology,2013,36(2):19-22.
[32]Joo H S,Hirai M,Shoda M.Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis no.4[J].Journal of Bioscience&Bioengineering.2005,100(2):184-191.
[33]Jin C P,Cheng K.Delftia tsuruhatensis strain KHN-HT01 16Sribosomal RNA gene,partial sequence,GenBank:MG543680.1[EB/OL].https://www.ncbi.nlm.nih.gov/nuccore/MG543680.1/,2018-12-01/2018-12-16.
[34]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002:258-284.State Environmental Protection Administration.Monitoring Method of Water and Waste Water[M],4th ed.Beijing:Chinese Environmental Science Press,2002:258-284.
[35]Jin C P.Unverified:Delftia tsuruhatensis SDS degradation transcriptional activation protein-like gene,partial sequence,GenBank:MH393620.1[EB/OL].https://www.ncbi.nlm.nih.gov/nuccore/MH393620,2018-12-04/2018-12-16.
[36]张若木,李泽琴,陈杨武,等.十二烷基硫酸钠微生物降解研究进展[J].再生资源与循化经济,2016,9(1):34-40.Zhang R M,Li Z Q,Chen Y W,et al.Research advances on microbial degradation of sodium dodecyl sulfate[J].Recyclable Resources and Cyclecar Economy,2016,9(1):34-40.
[37]崔文娟,董琳茜,孙珮石,等.硝化菌的分离及特性分析[J].环境工程学报,2012,6(2):683-686.Cui W J,Dong Li Q,Sun P S,et al.Isolation and characterization of nitrifiers[J].Chinese Journal of Environmental Engineering,2012,6(2):683-686.
[38]何厚波,熊杨,周敬超.生活垃圾填埋场渗滤液的特点及处理技术[J].环境卫生工程,2002,10(4):159-163.He H B,Xiong Y,Zhou J C.The characteristic and treatment technology of municipal solid waste landfill leachate[J].Environmental Sanitation Engineering,2002,10(4):159-163.
[39]董春松,樊耀波,李刚,等.我国垃圾渗滤液的特点和处理技术探讨[J].中国给水排水,2005,21(12):27-31.Dong C S,Fan Y B,Li G,et al.Characteristics of domestic refuse landfill leachate and discussion on treatment technology[J].China Water&Wastewater,2005,21(12):27-31.
[40]陈雷,贺磊,吴立群,等.垃圾渗滤液的处理现状及发展方向[J].环境工程,2016,34(增刊):295-298.Chen L,He L,Wu L Q,et al.Status of landfill leachate treatment and its development direction[J].Environmental Engineering,2016,34(Supplements):295-298.
[41]张培玉,曲洋,于德爽,等.菌株qy37的异养硝化/好氧反硝化机制比较及氨氮加速降解特性研究[J].环境科学,2010,31(8):1819-1826.Zhang P Y,Qu Y,Yu D S,et al.Comparison of heterotrophic nitrification and aerobic denitrification system by strain qy37 and its accelerating removal characteristic of NH4+-N[J].Environmental Science,2010,31(8):1819-1826.
[42]王欣,黄瑞敏,陈克复.制革废水氨氮处理的研究进展[J].皮革化工,2004,21(2):9-13.Wang X,Huang R M,Chen K F.Advances in ammonium removal of tannery wastewater[J].Leather Chemicals,2004,21(2):9-13.
[43]冯景伟,孙亚兵,郑正,等.制革废水处理技术研究进展[J].环境科学与技术,2008,31(6):73-77.Feng J W,Sun Y B,Zheng Z,et al.Recent advances of technology for treatment of tannery wastewater[J].Environmental Science&Technology,2008,31(6):73-77.
[44]游伟民.皮革废水治理技术的研究进展[J].皮革与化工,2009,26(2):16-20.You W M.Research progress in techniques of tannery wastewater treatment[J].Leather and Chemicals,2009,26(2):16-20.