HPLC法测定麸炒枳壳中柚皮苷的含量及其临床用量分析
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摘要
目的研究麸炒炮制后枳壳中橙皮苷含量的变化并确定其临床用量。方法含量测定采用HPLC法,使用Agilent 1200高效液相色谱仪,Agilent SB C_(18)色谱柱(4.6×250,5μm),甲醇-0.2%冰醋酸为流动相,梯度洗脱,流速1.0mL·min~(-1),柱温25℃,进样量10μL,检测波长320nm;根据测得麸炒枳壳中柚皮苷含量及相应批次药材含量计算转移率及临床用量。结果 10批麸炒枳壳中,橙皮苷的含量在0.980～1.928mg·g~(-1)范围内,对应转移率分别为45.36～89.06%,即每g饮片相当于1.33g生品。结论本研究所用方法能够准确、高效地分析麸炒枳壳中柚皮苷的含量并计算转移率,临床用量分析结果显示,麸炒炮制后枳壳较生品橙皮苷含量存在显著变化。
OBJECTIVE To investigate variation of hesperidin in Bran fried Fructus aurantii and determine its clinical dosage.METHODS An Agilent 1200 high performance liquid chromatography equipped with Agilent SB C_(18) column(4.6×250,5μm) was used for content determination,the mobile phase was methanol-0.2% glacial acetic acid in gradient elution,the flow rate was 1.0 mL·min~(-1),the column temperature was 25℃,inject volume was 10μL,and the detection wavelength was 320 nm;the obtained content was introduced to calculate transition rate of Bran fried Fructus aurantii with its crude material,and the clinical dosage was finally reckoned.RESULTS In 10 batches of Bran fried Fructus aurantii,the hesperidin content lied in the range of 0.980～1.928 mg·g~(-1),with the transition rate between 45.36～89.06%,indicating 1 g of the processed product was equivalent to 1.33 g of crude drug.CONCLUSION The method used was capable of determining hesperidin content in Bran fried Fructus aurantii and calculating transition rate;the results of clinical dosage showed that processing the crude drug brought about significant change in hesperidin content.
OBJECTIVE To investigate variation of hesperidin in Bran fried Fructus aurantii and determine its clinical dosage.METHODS An Agilent 1200 high performance liquid chromatography equipped with Agilent SB C_(18) column(4.6×250,5μm) was used for content determination,the mobile phase was methanol-0.2% glacial acetic acid in gradient elution,the flow rate was 1.0 mL·min~(-1),the column temperature was 25℃,inject volume was 10μL,and the detection wavelength was 320 nm;the obtained content was introduced to calculate transition rate of Bran fried Fructus aurantii with its crude material,and the clinical dosage was finally reckoned.RESULTS In 10 batches of Bran fried Fructus aurantii,the hesperidin content lied in the range of 0.980～1.928 mg·g~(-1),with the transition rate between 45.36～89.06%,indicating 1 g of the processed product was equivalent to 1.33 g of crude drug.CONCLUSION The method used was capable of determining hesperidin content in Bran fried Fructus aurantii and calculating transition rate;the results of clinical dosage showed that processing the crude drug brought about significant change in hesperidin content.
引文
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[2]范蕾,吴查青,王伟影,等.市售枳壳饮片的质量调查与指纹图谱研究[J].中国现代应用药学,2015,32(4):432-436.
[3]张栋健,李薇,何庆文,等.UHPLC-Q-TOF-MS分析枳壳炮制前后成分变化[J].中国中药杂志,2016,41(11):2070-2080.
[4]李正红,夏放高,陈海芳,等.枳壳麸炒前、后主要活性成分的含量变化[J].中国实验方剂学杂志,2013,19(19):18-21.
[5]陈向东,李荣,孙冬梅,等.麸炒枳壳配方颗粒HPLC指纹图谱[J].中国实验方剂学杂志,2013,19(3):100-103.
[6]徐以亮.麸炒枳壳配方颗粒特征图谱研究[J].中国药业,2012,21(22):46-47.
[7]曹君,龚千锋,肖兵华,等.不同产地枳壳麸炒品HPLC指纹图谱研究[J].中国中药杂志,2016,31(4):301-303.
[8]钱俊臻,王伯初.橙皮苷的药理作用研究进展[J].天然产物研究与开发,2010,22:176-180.