高温胁迫对滇重楼生理指标的影响
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摘要
目的探究滇重楼对高温抗性适应能力,为滇重楼在中南地区规模化引种栽培提供依据。方法以滇重楼生长健壮苗为实验材料,在25℃/20℃(昼温/夜温)、33℃/28℃(昼温/夜温)、39℃/34℃(昼温/夜温)的不同温度条件下连续胁迫处理7 d,测定其叶片生理指标,并进行显著性与相关性分析。结果随着温度的升高与胁迫时间的持续延长,在33℃/28℃与39℃/34℃下处理7 d后,滇重楼叶相对含水量明显下降,分别比对照(25℃/20℃)低17.38%、58.25%;相对电导率与丙二醛(MDA)含量均持续上升,其中39℃/34℃处理下上升幅度最大,电导率比对照高3.59倍,MDA含量比对照高2.55倍;39℃/34℃处理下可溶性糖含量逐渐下降,比对照低33.25%;可溶性蛋白含量均呈波浪状变化,33℃/28℃处理组波动幅度较大,7 d后上升为对照的1.32倍;处理组超氧化物歧化酶(SOD)活性均先升高后下降,39℃/34℃处理组4 d后达到峰值,比对照高2.35倍;处理组过氧化物酶(POD)活性均逐渐上升,39℃/34℃处理组4 d后上升为对照的2.33倍,之后变化幅度逐渐减缓。结论温度对滇重楼的各项生理指标均有明显的影响,温度为25℃/20℃条件下,植物生长状态良好,为适宜生长温度,33℃/28℃温度条件下,长时间的持续胁迫处理会引起植株叶片损伤,39℃/34℃持续高温胁迫则会严重损伤植株生理活性与形态结构,严重时甚至会导致植株死亡。
Objective To provide a reference for the introduction and cultivation of Paris polyphylla var. yunnanensis in mid-south region of China, its resistance adaptation ability of high temperature was explored. Methods Strong seedlings of P. polyphylla var. yunnanensis were chosen and treated under different temperature conditions(25 ℃/20 ℃, 33 ℃/28 ℃, 39 ℃/34 ℃) for 7 d, and then investigated the effect on their related physiological indexes. Results The results showed that with the increase of temperature and stress time, the relative water content of the leaves of P. polyphylla var. yunnanensis decreased significantly under the treatment of 33 ℃/28 ℃ and 39 ℃/34 ℃ for 7 d, which was 17.38% and 58.25% lower than the control(25 ℃/20 ℃) respectively. The relative conductivity and the content of malondialdehyde(MDA) were both increased continuously, and it was respectively 3.59 times and 2.55 times higher than that of control under the temperature of 39 ℃/34 ℃. The content of soluble sugar decreased gradually at 39 ℃/34 ℃, which was 33.25% lower than that of the control. The changing of soluble protein content showed a wavy trend, and the fluctuation range of 33 ℃/28 ℃ treatment groups was larger than that of the control group, which increased to 1.32 times of the control after 7 d. The SOD increased firstly and then decreased. The peak value was reached after 4 d at 39 ℃/34 ℃, which was 2.35 times higher than that of the control group. The POD increased gradually in treatment group, which increased to 2.33 times of that in control group at 39 ℃/34 ℃ for 4 d, and the amplitude of the change gradually slowed down. Conclusion In the experimental temperature range, P. polyphylla var. yunnanensis grew well at 25 ℃/20 ℃, which was its optimal growth temperature. Long-term continuous temperature stress treatment at 33 ℃/28 ℃ can cause damage to plant leaves; High temperature at 39 ℃/34 ℃will seriously damage the physiological and morphological structure of the plant, and even lead to the death of the plant.
Objective To provide a reference for the introduction and cultivation of Paris polyphylla var. yunnanensis in mid-south region of China, its resistance adaptation ability of high temperature was explored. Methods Strong seedlings of P. polyphylla var. yunnanensis were chosen and treated under different temperature conditions(25 ℃/20 ℃, 33 ℃/28 ℃, 39 ℃/34 ℃) for 7 d, and then investigated the effect on their related physiological indexes. Results The results showed that with the increase of temperature and stress time, the relative water content of the leaves of P. polyphylla var. yunnanensis decreased significantly under the treatment of 33 ℃/28 ℃ and 39 ℃/34 ℃ for 7 d, which was 17.38% and 58.25% lower than the control(25 ℃/20 ℃) respectively. The relative conductivity and the content of malondialdehyde(MDA) were both increased continuously, and it was respectively 3.59 times and 2.55 times higher than that of control under the temperature of 39 ℃/34 ℃. The content of soluble sugar decreased gradually at 39 ℃/34 ℃, which was 33.25% lower than that of the control. The changing of soluble protein content showed a wavy trend, and the fluctuation range of 33 ℃/28 ℃ treatment groups was larger than that of the control group, which increased to 1.32 times of the control after 7 d. The SOD increased firstly and then decreased. The peak value was reached after 4 d at 39 ℃/34 ℃, which was 2.35 times higher than that of the control group. The POD increased gradually in treatment group, which increased to 2.33 times of that in control group at 39 ℃/34 ℃ for 4 d, and the amplitude of the change gradually slowed down. Conclusion In the experimental temperature range, P. polyphylla var. yunnanensis grew well at 25 ℃/20 ℃, which was its optimal growth temperature. Long-term continuous temperature stress treatment at 33 ℃/28 ℃ can cause damage to plant leaves; High temperature at 39 ℃/34 ℃will seriously damage the physiological and morphological structure of the plant, and even lead to the death of the plant.
引文
[1]李恒.重楼属植物[M].北京:科学出版社,1998.
[2]中国科学院昆明植物研究所.云南植物志[M].北京:科学出版社,1997.
[3]颜璐璐,张艳军,高文远,等.滇重楼皂苷对10种肿瘤细胞株的细胞毒谱及构效关系研究[J].中国中药杂志,2008,33(6):2057-2060.
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[5]Chan J Y,Koon J C,Liu X,et al.Polyphyllin D,a steroidal saponin from Paris polyphylla inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo[J].J Ethnopharmacol,2011,137(1):64-69.
[6]Wang Y C,Yi T Y,Lin K H,et al.In vitro activity of Paris polyphylla smith against enterovirus 71 and coxsackievirus B3 and its immune modulation[J].Amer J Chin Med,2011,39(6):1219-1234.
[7]周浓,李自勤,姜北,等.超声波对滇重楼种子萌发能力的影响[J].湖北农业科学,2012,51(1):114-116.
[8]张琳,李海峰,张德全,等.层积和赤霉素协同作用对解除滇重楼种子休眠的影响[J].中国药学杂志,2013,48(20):1719-1724.
[9]张朝阳,赵庭周.重楼资源再生策略及其关键技术环节探讨[J].中草药,2009,40(2):319-323.
[10]苏菲菲,卢木娣,曾惠红,等.重楼总苷I对人视网膜色素上皮细胞系ARPE-19增殖、迁移的影响[J].安徽医科大学学报,2016,51(7):956-960.
[11]张珂,邓清华,马胜林.重楼醇提取物对胃癌SGC-7901细胞增殖和凋亡的影响[J].中华中医药刊,2016,34(1):145-148.
[12]刘涛,王玲,李玛,等.不同温度对滇重楼光合作用及有效成分含量的影响[J].中国现代中药,2015,17(10):1041-1043.
[13]太光聪.滇重楼的特征特性及栽培技术[J].现代农业科技,2012(4):171-172.
[14]李运昌.重楼属植物引种栽培的研究——I.滇重楼的有性繁殖试验初报[J].云南植物研究,1982,4(4):429-431.
[15]王丽萍,起学伟.云南重楼野生驯化及栽培技术研究初探[J].中国野生植物资源,2002,21(1):62-63.
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[17]张金渝,虞泓,张时刚,等.滇重楼与华重楼的野生驯化和繁殖技术研究[J].西南农业学报,2004,17(3):314-317.
[18]袁小坦.滇重楼与华重楼的引种驯化栽培研究初报[J].福建热作科技,2013,38(4):17-19.
[19]陈翠,汤王外,谭敬菊,等.不同遮荫方式及遮光度对滇重楼生长的影响研究[J].中国农学通报,2010,26(10):149-151.
[20]辛雅芬,石玉波.高温对八仙花生理指标的影响[J].安徽农业科学,2009,37(11):5204-5206.
[21]高俊凤.植物生理学实验技术[M].西安:世界图书出版社,1999.
[22]朱广廉.植物生理学试验指南[M].北京:北京大学出版社,1993.
[23]李合生.植物生理生化试验原理及技术[M].北京:高等教育出版社,2000.
[24]郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.
[25]刘凯歌,宋云鹏,龚繁荣,等.高温胁迫对甜椒幼苗生长和生理生化指标的影响[J].上海农业学报,2015,31(3):63-67.
[26]张恩平,张淑红,司龙亭.Na Cl胁迫对黄瓜幼苗子叶膜脂过氧化的影响[J].沈阳农业大学学报,2001,32(6):446-448.
[27]黄显波,严寒,胡建林,等.高温胁迫下水稻幼苗几个相关生理指标的变化[J].长江大学学报:自然科学版,2008,5(2):50-53.
[28]马英姿,张慧,王志毅,等.高温胁迫对蛇足石杉质膜透性及叶绿体结构的影响[J].中草药,2013,44(18):2605-2610.
[29]孙玉芳,王三根,尹丽,等.高温胁迫对黄连生理特性的影响研究[J].中国农学通报,2006,22(4):236-238.
[30]谢晓金,李秉柏,程高峰,等.高温对不同水稻品种剑叶生理特性的影响[J].农业现代化研究,2009,30(4):483-486.
[31]王志勇,廖丽,林文雄.高温对高羊茅叶片中渗透调节物质的影响[J].江西农业大学学报,2007,29(2):176-186.
[32]曾德静,王铖,刘军,等.高温胁迫对海州常山形态和生理特性的影响[J].东北林业大学学报,2013,41(3):90-94.
[33]Noctor G,Foyer C H.Aseorbate and glutathione:Keeping active oxygen under control[J].Annu Rev Plant Physiol Plant Mol Biol,1998,49(49):249-279.
[34]王江英,吴斌,刘伟鑫,等.24种连蕊茶在夏季极端髙温干旱条件下的耐旱潜力评价[J].热带作物学报,2015,36(3):563-569.
[35]邢晓蕾,刘冬云,胡松竹.高温胁迫对兰州百合幼苗生理指标的影响[J].河北农业大学学报,2010,33(5):65-68.
[36]苏文华,张光飞.滇重楼光合作用与环境因子的关系[J].云南大学学报:自然科学版,2003,25(6):545-548.
[2]中国科学院昆明植物研究所.云南植物志[M].北京:科学出版社,1997.
[3]颜璐璐,张艳军,高文远,等.滇重楼皂苷对10种肿瘤细胞株的细胞毒谱及构效关系研究[J].中国中药杂志,2008,33(6):2057-2060.
[4]颜璐璐,张艳军,高文远,等.滇重楼皂苷成分体内外抗肺癌活性研究[J].中草药,2009,40(3):424-428.
[5]Chan J Y,Koon J C,Liu X,et al.Polyphyllin D,a steroidal saponin from Paris polyphylla inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo[J].J Ethnopharmacol,2011,137(1):64-69.
[6]Wang Y C,Yi T Y,Lin K H,et al.In vitro activity of Paris polyphylla smith against enterovirus 71 and coxsackievirus B3 and its immune modulation[J].Amer J Chin Med,2011,39(6):1219-1234.
[7]周浓,李自勤,姜北,等.超声波对滇重楼种子萌发能力的影响[J].湖北农业科学,2012,51(1):114-116.
[8]张琳,李海峰,张德全,等.层积和赤霉素协同作用对解除滇重楼种子休眠的影响[J].中国药学杂志,2013,48(20):1719-1724.
[9]张朝阳,赵庭周.重楼资源再生策略及其关键技术环节探讨[J].中草药,2009,40(2):319-323.
[10]苏菲菲,卢木娣,曾惠红,等.重楼总苷I对人视网膜色素上皮细胞系ARPE-19增殖、迁移的影响[J].安徽医科大学学报,2016,51(7):956-960.
[11]张珂,邓清华,马胜林.重楼醇提取物对胃癌SGC-7901细胞增殖和凋亡的影响[J].中华中医药刊,2016,34(1):145-148.
[12]刘涛,王玲,李玛,等.不同温度对滇重楼光合作用及有效成分含量的影响[J].中国现代中药,2015,17(10):1041-1043.
[13]太光聪.滇重楼的特征特性及栽培技术[J].现代农业科技,2012(4):171-172.
[14]李运昌.重楼属植物引种栽培的研究——I.滇重楼的有性繁殖试验初报[J].云南植物研究,1982,4(4):429-431.
[15]王丽萍,起学伟.云南重楼野生驯化及栽培技术研究初探[J].中国野生植物资源,2002,21(1):62-63.
[16]唐吉鸿,李光美,郑贤庄.野生滇重楼驯化栽培技术[J].现代农业科技,2012(5):172.
[17]张金渝,虞泓,张时刚,等.滇重楼与华重楼的野生驯化和繁殖技术研究[J].西南农业学报,2004,17(3):314-317.
[18]袁小坦.滇重楼与华重楼的引种驯化栽培研究初报[J].福建热作科技,2013,38(4):17-19.
[19]陈翠,汤王外,谭敬菊,等.不同遮荫方式及遮光度对滇重楼生长的影响研究[J].中国农学通报,2010,26(10):149-151.
[20]辛雅芬,石玉波.高温对八仙花生理指标的影响[J].安徽农业科学,2009,37(11):5204-5206.
[21]高俊凤.植物生理学实验技术[M].西安:世界图书出版社,1999.
[22]朱广廉.植物生理学试验指南[M].北京:北京大学出版社,1993.
[23]李合生.植物生理生化试验原理及技术[M].北京:高等教育出版社,2000.
[24]郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.
[25]刘凯歌,宋云鹏,龚繁荣,等.高温胁迫对甜椒幼苗生长和生理生化指标的影响[J].上海农业学报,2015,31(3):63-67.
[26]张恩平,张淑红,司龙亭.Na Cl胁迫对黄瓜幼苗子叶膜脂过氧化的影响[J].沈阳农业大学学报,2001,32(6):446-448.
[27]黄显波,严寒,胡建林,等.高温胁迫下水稻幼苗几个相关生理指标的变化[J].长江大学学报:自然科学版,2008,5(2):50-53.
[28]马英姿,张慧,王志毅,等.高温胁迫对蛇足石杉质膜透性及叶绿体结构的影响[J].中草药,2013,44(18):2605-2610.
[29]孙玉芳,王三根,尹丽,等.高温胁迫对黄连生理特性的影响研究[J].中国农学通报,2006,22(4):236-238.
[30]谢晓金,李秉柏,程高峰,等.高温对不同水稻品种剑叶生理特性的影响[J].农业现代化研究,2009,30(4):483-486.
[31]王志勇,廖丽,林文雄.高温对高羊茅叶片中渗透调节物质的影响[J].江西农业大学学报,2007,29(2):176-186.
[32]曾德静,王铖,刘军,等.高温胁迫对海州常山形态和生理特性的影响[J].东北林业大学学报,2013,41(3):90-94.
[33]Noctor G,Foyer C H.Aseorbate and glutathione:Keeping active oxygen under control[J].Annu Rev Plant Physiol Plant Mol Biol,1998,49(49):249-279.
[34]王江英,吴斌,刘伟鑫,等.24种连蕊茶在夏季极端髙温干旱条件下的耐旱潜力评价[J].热带作物学报,2015,36(3):563-569.
[35]邢晓蕾,刘冬云,胡松竹.高温胁迫对兰州百合幼苗生理指标的影响[J].河北农业大学学报,2010,33(5):65-68.
[36]苏文华,张光飞.滇重楼光合作用与环境因子的关系[J].云南大学学报:自然科学版,2003,25(6):545-548.