高效液相色谱多波长法同时测定中风合剂中梓醇、哈巴苷、绿原酸和芍药苷含量
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摘要
目的建立同时测定中风合剂中梓醇、哈巴苷、绿原酸和芍药苷4种有效成分含量的高效液相色谱多波长法。方法色谱柱为Welch XB-C18柱(250 mm×4. 6 mm,5μm),流动相A为0. 06%磷酸溶液、B为乙腈(梯度洗脱),流速为1. 0 m L/min,检测波长分别为200,231,327 nm,柱温为30℃,进样量为10μL。结果梓醇、哈巴苷、绿原酸、芍药苷进样量分别在0. 156～1. 248μg,0. 154～1. 232μg,0. 136～1. 086μg,0. 231～1. 845μg范围内与峰面积线性关系良好(r=0. 999 9,0. 999 9,1. 000 0,0. 999 9),平均加样回收率分别为99. 47%,99. 54%,99. 89%,99. 94%,RSD分别为0. 54%,0. 91%,0. 23%,0. 35%(n=6)。结论该方法操作简单、专属性强,可用于中风合剂的质量控制。
Objective To establish a multiwavelength HPLC method for simultaneous determination of 4 active components of catalpol,harpagide,chlorogenic acid and paeoniflorin in Zhongfeng Mixture. Methods The chromatographic column was Welch XB-C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase A was 0. 06% phosphoric acid solution,the mobile phase B was acetonitrile( gradient elution),the flow rate was 1. 0 m L/min,the detection wavelength was 200,231,327 nm,the column temperature was 30 ℃ and the sample size was 10 μL. Results The linear range was 0. 156-1. 248 μg( r = 0. 999 9) for catalpol,0. 154-1. 232 μg( r = 0. 999 9)for harpagide,0. 136-1. 086 μg( r = 1. 000 0) for chlorogenic acid and 0. 231-1. 845 μg( r = 0. 999 9) for paeoniflorin. The average recoveries were 99. 47%,99. 54%,99. 89%,99. 94%,RSDs were 0. 54%,0. 91%,0. 23% and 0. 35%( n = 6). Conclusion The method is simple and specific,which can be used for the quality control of Zhongfeng Mixture.
Objective To establish a multiwavelength HPLC method for simultaneous determination of 4 active components of catalpol,harpagide,chlorogenic acid and paeoniflorin in Zhongfeng Mixture. Methods The chromatographic column was Welch XB-C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase A was 0. 06% phosphoric acid solution,the mobile phase B was acetonitrile( gradient elution),the flow rate was 1. 0 m L/min,the detection wavelength was 200,231,327 nm,the column temperature was 30 ℃ and the sample size was 10 μL. Results The linear range was 0. 156-1. 248 μg( r = 0. 999 9) for catalpol,0. 154-1. 232 μg( r = 0. 999 9)for harpagide,0. 136-1. 086 μg( r = 1. 000 0) for chlorogenic acid and 0. 231-1. 845 μg( r = 0. 999 9) for paeoniflorin. The average recoveries were 99. 47%,99. 54%,99. 89%,99. 94%,RSDs were 0. 54%,0. 91%,0. 23% and 0. 35%( n = 6). Conclusion The method is simple and specific,which can be used for the quality control of Zhongfeng Mixture.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:105,117,124,193.
[2]杨辉.双波长UPLC法同时测定艾滋病口腔含漱液中梓醇、哈巴苷和哈巴俄苷的含量[J].中国药房,2015,26(24):3414-3416.
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[4]季宁平,徐晓秋,傅超美,等. HPLC法同时测定艾附暖宫丸中芍药苷、阿魏酸和川断续皂苷Ⅵ[J].中成药,2015,37(3):534-537.
[5]周佳,齐红艺. HPLC-MS/MS法同时测定玄参中8个成分的含量[J].中国现代中药,2017,19(5):670-674.
[6]印酬,向红霞,王洪凤,等.贵州不同地区山银花中4种异绿原酸含量测定方法的建立[J].贵州医科大学学报,2016,41(10):1176-1180.
[7]张申亮,聂黎行. HPLC法同时测定小儿清咽颗粒中5种成分的含量[J].中国药房,2017,28(21):3004-3007.
[8]何建雄,赖小平,魏刚,等. HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷[J].中国实验方剂学杂志,2011,17(6):48-50.
[9]梁化菊.桃红四物汤水提液中芍药苷及梓醇含量检测分析[J].中国处方药,2015,13(10):23-24.
[2]杨辉.双波长UPLC法同时测定艾滋病口腔含漱液中梓醇、哈巴苷和哈巴俄苷的含量[J].中国药房,2015,26(24):3414-3416.
[3]龚友兰,向大雄,邓长凤,等. HPLC-UV双波长法同时测定玄参中5种主要成分的含量[J].中南药学,2008,6(6):660-663.
[4]季宁平,徐晓秋,傅超美,等. HPLC法同时测定艾附暖宫丸中芍药苷、阿魏酸和川断续皂苷Ⅵ[J].中成药,2015,37(3):534-537.
[5]周佳,齐红艺. HPLC-MS/MS法同时测定玄参中8个成分的含量[J].中国现代中药,2017,19(5):670-674.
[6]印酬,向红霞,王洪凤,等.贵州不同地区山银花中4种异绿原酸含量测定方法的建立[J].贵州医科大学学报,2016,41(10):1176-1180.
[7]张申亮,聂黎行. HPLC法同时测定小儿清咽颗粒中5种成分的含量[J].中国药房,2017,28(21):3004-3007.
[8]何建雄,赖小平,魏刚,等. HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷[J].中国实验方剂学杂志,2011,17(6):48-50.
[9]梁化菊.桃红四物汤水提液中芍药苷及梓醇含量检测分析[J].中国处方药,2015,13(10):23-24.