夏南牛犊牛口蹄疫母源抗体衰减变化试验研究
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摘要
[目的]探讨夏南牛犊牛口蹄疫母源抗体衰减变化规律,为犊牛口蹄疫科学适时免疫提供参考依据。[方法]血样采集使用一次性真空采血管,抗体检测采用液相阻断ELISA检测方法,抗体效价判定依据畜牧兽医主管部门动物疫病监测方案。[结果]母牛产后、犊牛出生当天,O型抗体效价合格率达100%,A型抗体效价合格率达92%。O型口蹄疫第4周抗体合格率达86.96%,到第5周已降至66.67%;A型口蹄疫第3周抗体合格率达75%,第4周抗体合格率已降至39.13%。牛口蹄疫O型疫苗抗体衰减时间稍长于A型。[结论]犊牛获得母源抗体的保护:O型口蹄疫4周内有较强的保护作用,A型口蹄疫3周内有较强的保护作用。建议夏南牛犊牛口蹄疫首免时间在出生后40 d左右为宜。
[Objective] To investigate the variation of maternal antibody attenuation for foot-and-mouth disease in Xia'nan calf, and to provide reference for scientific and timely immunization against foot-and-mouth disease in calf. [Method] Blood samples were collected by one-time vacuum blood vessel collection, antibody detection was performed by liquid phase blocking ELISA method, and antibody titer was determined according to animal disease surveillance program of animal husbandry and veterinary authorities. [Results] The qualified rate of O-type antibody and A-type antibody was 100% and 92% respectively on postpartum cow and calf birth day. The qualified rate of antibodies against foot-and-mouth disease type O reached 86.96% in the fourth week and 66.67% in the fifth week. The qualified rate of antibodies against foot-and-mouth disease type A reached 75% in the third week and 39.13% in the fourth week. The attenuation time of antibody of type O vaccine was slightly longer than that of type A vaccine. [Conclusion] Protection of calves against mother-derived antibodies: Type O had a strong protective effect within four weeks and a strong protective effect within three weeks. It is suggested that the first immunization time of foot-and-mouth disease in Xia'nan calves should be around 40 days after birth.
[Objective] To investigate the variation of maternal antibody attenuation for foot-and-mouth disease in Xia'nan calf, and to provide reference for scientific and timely immunization against foot-and-mouth disease in calf. [Method] Blood samples were collected by one-time vacuum blood vessel collection, antibody detection was performed by liquid phase blocking ELISA method, and antibody titer was determined according to animal disease surveillance program of animal husbandry and veterinary authorities. [Results] The qualified rate of O-type antibody and A-type antibody was 100% and 92% respectively on postpartum cow and calf birth day. The qualified rate of antibodies against foot-and-mouth disease type O reached 86.96% in the fourth week and 66.67% in the fifth week. The qualified rate of antibodies against foot-and-mouth disease type A reached 75% in the third week and 39.13% in the fourth week. The attenuation time of antibody of type O vaccine was slightly longer than that of type A vaccine. [Conclusion] Protection of calves against mother-derived antibodies: Type O had a strong protective effect within four weeks and a strong protective effect within three weeks. It is suggested that the first immunization time of foot-and-mouth disease in Xia'nan calves should be around 40 days after birth.
引文
[1] 刘贺.O型口蹄疫疫苗免疫牛抗体消长动态规律[J].畜牧与兽医,2016,48(10):100-102.
[2] 郭爱珍,殷宏,张继瑜,等.肉牛常见病防制图册[M].北京:中国农业科学技术出版社,2013.
[2] 郭爱珍,殷宏,张继瑜,等.肉牛常见病防制图册[M].北京:中国农业科学技术出版社,2013.