基于非线性动力学方法研究钙离子在针刺神经电信号传导中的作用
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摘要
目的研究钙离子(Ca~(2+))对针刺神经电信号的影响,为揭示Ca~(2+)在针刺信号传导过程中的作用及针效产生的始动机制进一步提供实验依据。方法选用健康成年雄性SD大鼠,实验组采用0.1 mol/L乙二醇双乙胺醚四乙酸(EGTA)5mL穴位注射络合足三里穴区Ca~(2+),对照组采用0.05 mol/L Ca~(2+)-EGTA对照液5mL穴位注射。分别记录穴位注射前(YQ)及穴位注射后即刻(YH0)、7 min(YH7)、14 min(YH14)、21 min(YH21)、28 min(YH28)时神经细束的放电情况。应用非线性时间序列分析方法、小波能量熵分析法、峰峰间期等方法对采集的数据进行分析。结果实验组各时间段(YH0、YH21、YH28)的放电频率均降低,与同组YQ比较差异均具有统计学意义(P<0.01);对照组各时间段(YH14、YH21、YH28)的放电频率显著增高,与同组YQ比较差异均具有统计学意义(P<0.05)。实验组YH21、YH28的放电频率与对照组比较差异均具有统计学意义(P<0.05)。通过放电频率、波形幅值的统计发现穴区Ca~(2+)浓度降低对针刺引发的放电序列有比较明显的影响。结论 Ca~(2+)是针效产生机制的重要环节之一,其在针刺神经电信号传导过程中起着重要作用。
Objective To investigate the effect of calcium ions(Ca~(2+)) on electroneurographic signals of acupuncture, and to provide experimental evidence to reveal the effect of Ca~(2+) on the process of signal transduction of acupuncture and the initial mechanism of acupuncture effect. Method Healthy adult male Sprague-Dawley(SD) rats were selected. The experimental group received acupoint injection of 5 mL ethyleneglycol-bis-(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid(EGTA)(0.1 mol/L) to chelate Ca~(2+) in the area of Zusanli(ST36), while the control group received acupoint injection of 5 mL Ca~(2+)-EGTA(0.05 mol/L). The electrical discharge of nerve fiber bundle was respectively recorded before acupoint injection(YQ), immediately after acupoint injection(YH0), and at 7 min(YH7), 14 min(YH14), 21 min(YH21) and 28 min(YH28) after acupoint injection. The collected data were analyzed by nonlinear time series analysis, wavelet energy entropy analysis and interspike intervals. Result In the experimental group, the discharge frequency at different time points(YH0, YH21 and YH28) decreased, and was significantly different from that at YQ(P<0.01). In the control group, the discharge frequency at different time points(YH14, YH21 and YH28) increased, and was significantly different from that at YQ(P<0.05). There was a significant difference in the discharge frequency at different time points(YH21 and YH28) between the two groups(P<0.05). The reduction of Ca~(2+)concentration in the area of acupoint had a relatively obvious effect on the discharge sequence caused by acupuncture according to the statistics of the discharge frequency and the waveform amplitude. Conclusion Ca~(2+) is one of the important links in the mechanism of acupuncture effect, as it plays an important role in the process of electroneurographic signal transduction of acupuncture.
Objective To investigate the effect of calcium ions(Ca~(2+)) on electroneurographic signals of acupuncture, and to provide experimental evidence to reveal the effect of Ca~(2+) on the process of signal transduction of acupuncture and the initial mechanism of acupuncture effect. Method Healthy adult male Sprague-Dawley(SD) rats were selected. The experimental group received acupoint injection of 5 mL ethyleneglycol-bis-(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid(EGTA)(0.1 mol/L) to chelate Ca~(2+) in the area of Zusanli(ST36), while the control group received acupoint injection of 5 mL Ca~(2+)-EGTA(0.05 mol/L). The electrical discharge of nerve fiber bundle was respectively recorded before acupoint injection(YQ), immediately after acupoint injection(YH0), and at 7 min(YH7), 14 min(YH14), 21 min(YH21) and 28 min(YH28) after acupoint injection. The collected data were analyzed by nonlinear time series analysis, wavelet energy entropy analysis and interspike intervals. Result In the experimental group, the discharge frequency at different time points(YH0, YH21 and YH28) decreased, and was significantly different from that at YQ(P<0.01). In the control group, the discharge frequency at different time points(YH14, YH21 and YH28) increased, and was significantly different from that at YQ(P<0.05). There was a significant difference in the discharge frequency at different time points(YH21 and YH28) between the two groups(P<0.05). The reduction of Ca~(2+)concentration in the area of acupoint had a relatively obvious effect on the discharge sequence caused by acupuncture according to the statistics of the discharge frequency and the waveform amplitude. Conclusion Ca~(2+) is one of the important links in the mechanism of acupuncture effect, as it plays an important role in the process of electroneurographic signal transduction of acupuncture.
引文
[1]Zheng J,Zeng XH,Wang SQ.Calcium ion as cellular messenger[J].Science China Life Sciences,2015,58(1):1-5.
[2]于玮,苏兴利.钙离子和神经系统环路的整合作用[J].国外医学(医学地理分册),2008,29(1):9-13.
[3]姚凯,郭义,胡利民,等.针刺镇痛与中枢神经系统神经元内外游离钙离子浓度的关系[J].天津中医药,2005,22(5):399-400.
[4]郭义,张艳军,王秀云,等.络合足三里穴Ca2+对针刺足三里抑制家兔亢进胃运动的影响[J].上海针灸杂志,1998,17(5):40-41.
[5]郭义,张艳军,王秀云,等.络合足三里穴Ca2+对针刺足三里抑制正常家兔胃运动影响的实验研究[J].针刺研究,1998,23(2):146-148.
[6]张艳军,郭义,王秀云,等.改变“内关”穴处Ca2+浓度对针刺“内关”治疗家兔实验性心律失常效应的影响[J].针刺研究,1995,20(2):63-67.
[7]沈德凯.关于腧穴针感形态结构的研究[J].中国医药学报,1989,4(4):57-64.
[8]薛明,王江,邓斌,等.基于广义线性模型的针刺足三里脊髓背根神经活动解码[J].物理学报,2013,62(9):483-492.
[9]萨喆燕,黄猛,张迪,等.手针刺激“足三里”穴局部肥大细胞活动与外周神经放电的相关性研究[J].针刺研究,2013,38(2):118-122.
[10]Uchida S,Shimura M,Ohsawa H,et al.Neural mechanism of bradycardiac responses elicited by acupuncture-like stimulation to a hind limb in anesthetized rats[J].J Physiol Sci,2007,57(6):377-382.
[11]李为民,陈颖波,王晓艳.手针和电针大鼠“足三里”穴位诱发的外周传人神经信号特征研究[J].针刺研究,2008,33(1):65-70.
[12]Men C,Wang J,Deng B,et al.Decoding acupuncture electrical signals in spinal dorsal root ganglion[J].Neurocomputing,2012,79(9):12-17.
[13]张保真,王俊明.人皮肤内神经-肥大细胞联接在经络线上的发现--Ⅰ.传出性神经-肥大细胞联接[J].神经解剖学杂志,1985,1(1):47-52.
[14]Liu YY,Guo Y,Guo YM,et al.Characteristics extraction and analysis on the electrical signals of spinal dorsal root nerve evoked by acupuncture manipulations[C].The 4th International Conference on Biomedical Engineering and Informatics,2011:680-684.
[15]冯加纯.细胞内钙超载和自由基与迟发性神经元坏死[J].中风与神经疾病杂志,1993,10(3):187-189.
[16]姚凯,郭义,胡利民,等.针刺对大鼠脑缺血超早期脑细胞内外游离钙离子浓度的影响[J].中国中西医结合急救杂志,2005,12(5):303-305.
[17]李忠仁.实验针灸学[M].第2版,北京:中国中医药出版社,2007:1-3.
[18]景丹丹,李忠仁.钙离子生物活性与针刺调节作用的研究进展[J].北京中医药,2008,27(8):663-665.
[19]张阔,陈波,赵雪,等.穴位是针刺信息传递的“放大器”[J].辽宁中医杂志,2015,42(1):166-167.
[20]白杨,郭义.针刺效应/信号调节网络初探[J].针刺研究,2013,38(4):330-333.
[21]李鑫举,赵雪,郭义,等.试论针刺反应与针刺效应的关系[J].中医学报,2017,32(1):155-158.
[22]郭义,马东明,张春煦,等.家兔足三里穴钙元素浓度的推挽灌流观察[J].上海针灸杂志,2003,22(7):26-27.
[23]郭义,徐汤苹,陈进生,等.家兔经穴钙离子浓度特异性的研究[J].针刺研究,1991,16(1):66-68.
[24]邓元江,易受乡,严洁,等.足阳明经穴针刺血清对家兔离体胃窦平滑肌细胞内钙离子浓度影响的实验研究[J].新中医,2005,37(10):91-93.
[25]易受乡,邓元江,严洁,等.针刺足阳明经穴对家兔胃窦平滑肌细胞舒缩长度及胞内钙离子浓度的影响[J].中国中医药科技,2005,12(1):1-3.
[26]裴莹,赵雪,李忠正,等.钙离子与肥大细胞在针刺效应产生中作用的研究[J].临床和实验医学杂志,2017,16(12):1145-1148.
[27]王俊玲,余谦,冯君平,等.牛磺酸对氟致神经细胞内钙离子浓度和细胞凋亡改变的干预研究[J].毒理学杂志,2016,30(3):189-191.
[28]庄严,时京,吕燕华,等.人参皂甙Rb1对香烟烟雾诱导的大鼠神经细胞凋亡和细胞内Ca2+浓度的影响[J].现代生物医学进展,2013,13(31):6019-6022.
[29]闫晓英,姜亚磊,狄烊,等.脑缺血损伤后Ca2+超载引起神经细胞凋亡的机制[J].吉林医药学院学报,2013,34(4):277-280.
[2]于玮,苏兴利.钙离子和神经系统环路的整合作用[J].国外医学(医学地理分册),2008,29(1):9-13.
[3]姚凯,郭义,胡利民,等.针刺镇痛与中枢神经系统神经元内外游离钙离子浓度的关系[J].天津中医药,2005,22(5):399-400.
[4]郭义,张艳军,王秀云,等.络合足三里穴Ca2+对针刺足三里抑制家兔亢进胃运动的影响[J].上海针灸杂志,1998,17(5):40-41.
[5]郭义,张艳军,王秀云,等.络合足三里穴Ca2+对针刺足三里抑制正常家兔胃运动影响的实验研究[J].针刺研究,1998,23(2):146-148.
[6]张艳军,郭义,王秀云,等.改变“内关”穴处Ca2+浓度对针刺“内关”治疗家兔实验性心律失常效应的影响[J].针刺研究,1995,20(2):63-67.
[7]沈德凯.关于腧穴针感形态结构的研究[J].中国医药学报,1989,4(4):57-64.
[8]薛明,王江,邓斌,等.基于广义线性模型的针刺足三里脊髓背根神经活动解码[J].物理学报,2013,62(9):483-492.
[9]萨喆燕,黄猛,张迪,等.手针刺激“足三里”穴局部肥大细胞活动与外周神经放电的相关性研究[J].针刺研究,2013,38(2):118-122.
[10]Uchida S,Shimura M,Ohsawa H,et al.Neural mechanism of bradycardiac responses elicited by acupuncture-like stimulation to a hind limb in anesthetized rats[J].J Physiol Sci,2007,57(6):377-382.
[11]李为民,陈颖波,王晓艳.手针和电针大鼠“足三里”穴位诱发的外周传人神经信号特征研究[J].针刺研究,2008,33(1):65-70.
[12]Men C,Wang J,Deng B,et al.Decoding acupuncture electrical signals in spinal dorsal root ganglion[J].Neurocomputing,2012,79(9):12-17.
[13]张保真,王俊明.人皮肤内神经-肥大细胞联接在经络线上的发现--Ⅰ.传出性神经-肥大细胞联接[J].神经解剖学杂志,1985,1(1):47-52.
[14]Liu YY,Guo Y,Guo YM,et al.Characteristics extraction and analysis on the electrical signals of spinal dorsal root nerve evoked by acupuncture manipulations[C].The 4th International Conference on Biomedical Engineering and Informatics,2011:680-684.
[15]冯加纯.细胞内钙超载和自由基与迟发性神经元坏死[J].中风与神经疾病杂志,1993,10(3):187-189.
[16]姚凯,郭义,胡利民,等.针刺对大鼠脑缺血超早期脑细胞内外游离钙离子浓度的影响[J].中国中西医结合急救杂志,2005,12(5):303-305.
[17]李忠仁.实验针灸学[M].第2版,北京:中国中医药出版社,2007:1-3.
[18]景丹丹,李忠仁.钙离子生物活性与针刺调节作用的研究进展[J].北京中医药,2008,27(8):663-665.
[19]张阔,陈波,赵雪,等.穴位是针刺信息传递的“放大器”[J].辽宁中医杂志,2015,42(1):166-167.
[20]白杨,郭义.针刺效应/信号调节网络初探[J].针刺研究,2013,38(4):330-333.
[21]李鑫举,赵雪,郭义,等.试论针刺反应与针刺效应的关系[J].中医学报,2017,32(1):155-158.
[22]郭义,马东明,张春煦,等.家兔足三里穴钙元素浓度的推挽灌流观察[J].上海针灸杂志,2003,22(7):26-27.
[23]郭义,徐汤苹,陈进生,等.家兔经穴钙离子浓度特异性的研究[J].针刺研究,1991,16(1):66-68.
[24]邓元江,易受乡,严洁,等.足阳明经穴针刺血清对家兔离体胃窦平滑肌细胞内钙离子浓度影响的实验研究[J].新中医,2005,37(10):91-93.
[25]易受乡,邓元江,严洁,等.针刺足阳明经穴对家兔胃窦平滑肌细胞舒缩长度及胞内钙离子浓度的影响[J].中国中医药科技,2005,12(1):1-3.
[26]裴莹,赵雪,李忠正,等.钙离子与肥大细胞在针刺效应产生中作用的研究[J].临床和实验医学杂志,2017,16(12):1145-1148.
[27]王俊玲,余谦,冯君平,等.牛磺酸对氟致神经细胞内钙离子浓度和细胞凋亡改变的干预研究[J].毒理学杂志,2016,30(3):189-191.
[28]庄严,时京,吕燕华,等.人参皂甙Rb1对香烟烟雾诱导的大鼠神经细胞凋亡和细胞内Ca2+浓度的影响[J].现代生物医学进展,2013,13(31):6019-6022.
[29]闫晓英,姜亚磊,狄烊,等.脑缺血损伤后Ca2+超载引起神经细胞凋亡的机制[J].吉林医药学院学报,2013,34(4):277-280.