野生软枣猕猴桃种子萌发及离体快繁技术研究
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摘要
试验以野生软枣猕猴桃的幼嫩茎段为外植体,对软枣猕猴桃无菌系建立、增殖培养、生根培养技术等快繁关键技术及种子萌发特性进行研究,以期为野生软枣猕猴桃资源的开发利用提供技术支持。结果表明,采用2.5%次氯酸钠浸泡10min再用0.1%升汞浸泡9min为最佳消毒方法,外植体的污染率和死亡率都比较低,分别是27.5%和17.5%;最佳增殖培养基为MS+6-BA 0.8mg/L+NAA 0.3mg/L,增殖系数达4.84;最佳生根培养基为1/2MS+IBA 1.0mg/L+NAA 0.1mg/L,根系健壮,生根率达到100%;种子在45℃恒温水中浸泡30min,然后用0.08%赤霉素处理24h发芽率最高,为70%。
For well utilization of the rich Actinidia arguta genetics resources,taking the young shoots A. arguta as explants,key technologies on its rapid propagation such as sterile materials,multiplication culture,rooting,and the characteristics of seed germination were studied. The results showed that: The best disinfection way for young shoots explants was soaking in sodium hypochlorite 2. 5% for 10 min,then Hg Cl20. 1% for 9 min,and the pollution rate and death rate was 27. 5% and 17. 5% respectively; The best medium for multiplication culture was MS+6-BA 0. 8 mg/L + 0. 3 mg/L NAA,and the multiplication coefficient was 4. 84; The best medium for rooting was 1/2 MS+IBA 1. 0 mg/L + NAA 0. 1 mg/L,roots robust and the rooting rate was 100%; The germination rate of seed was highest( 70%) when they were soaking in the water with 45℃ constant temperature for 30 min followed by 0. 08% GA for 24 h.
For well utilization of the rich Actinidia arguta genetics resources,taking the young shoots A. arguta as explants,key technologies on its rapid propagation such as sterile materials,multiplication culture,rooting,and the characteristics of seed germination were studied. The results showed that: The best disinfection way for young shoots explants was soaking in sodium hypochlorite 2. 5% for 10 min,then Hg Cl20. 1% for 9 min,and the pollution rate and death rate was 27. 5% and 17. 5% respectively; The best medium for multiplication culture was MS+6-BA 0. 8 mg/L + 0. 3 mg/L NAA,and the multiplication coefficient was 4. 84; The best medium for rooting was 1/2 MS+IBA 1. 0 mg/L + NAA 0. 1 mg/L,roots robust and the rooting rate was 100%; The germination rate of seed was highest( 70%) when they were soaking in the water with 45℃ constant temperature for 30 min followed by 0. 08% GA for 24 h.
引文
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[2]马月申,袁福贵,赵淑兰.软枣猕猴桃果实营养成分的测定[J].特产研究,1999(1):44-45.
[3]刘延吉,郝桂杰,姜岩岩,等.不同品种野生软枣猕猴桃最佳组培方法的研究[J].北方园艺,2012(17):124-126.
[4]龙茹,秘树青,王子华,等.外源激素对软枣猕猴桃硬枝扦插生根的影响[J].河北科技师范学院学报,2010,24(2):12-15.
[5]黄祥童,刘军,金哲军,等.软枣猕猴桃光雾扦插育苗试验[J].北华大学学报(自然科学版),2010,11(2):177-182.
[6]张伟庆,李红莉.软枣猕猴桃播种栽培技术初探[J].林业勘查设计,2009(3):100-101.
[7]曹坳程,郑建秋,郭美霞,等.土壤消毒技术及要点[J].蔬菜,2011(4):41-44.
[8]陈魁.实验设计与分析[M].北京:清华大学出版社,2005:72-138,276-293.
[9]刘文卿.实验设计[M].北京:清华大学出版社,2005:64-100,103-133,222-229.
[10]赵选民.实验设计方法[M].北京:科学出版社,2006:64-118,169-189.
[11]李云雁,胡传荣.试验设计与数据处理[M].北京:化学工业出版社&教材出版中心,2005:79-121,208-227.
[12]黄文江,周守标,阚显照,等.越橘属植物克隆的体外繁殖[J].中国野生植物资源,2005,24(2):62-64.
[13]贾景明,马纯艳,郑国,等.软枣猕猴桃体细胞培养获得再生植株[J].沈阳师范学院学报(自然科学版),1999(4):63-65.
[14]胡皓,张志东.软枣猕猴桃魁绿品种组培微繁技术研究[J].吉林农业,2011(2):71-72.