国槐根茎腐烂病病原菌的形态与分子鉴定
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摘要
为了确定引起国槐根茎腐烂病的病原菌种类,以国槐(Sophora japonica Linn.)典型根茎腐烂病病株为试材,采用组织分离法获得纯菌株,并对所得菌株进行致病性测定、形态学鉴定及rDNA-ITS序列分析,研究了国槐根茎腐烂病的病原菌种类。根据24个菌株的培养性状和形态特征,鉴定所分离菌株均为镰刀菌。致病性测定表明,24个菌株均能侵染寄主树皮组织使其腐烂变黑褐色。在Gen Bank序列数据库中,24个菌株的DNA序列分别与多隔镰刀菌(Fusarium decemcellulare)、层生镰刀菌(F.proliferatum)、木贼镰刀菌(F.equiseti)、F.keratoplasticum和腐皮镰刀菌(F.solani)的ITS序列同源性为99%~100%。
With Sophora japonica Linn.root rot,the pathogens were isolated by tissue separation,tested by the standard Koch's postulation methods,and identified by the methods of microscopy and molecules,and the pathogen species of Chinese scholartree root rot were studied.From the culture characters and morphological characteristics of the 24 strains,the isolated strains were identified as Fusarium sp.These strains of Fusarium could infect in vivo Chinese scholartree barkes and make it black brown and rot.In GenB ank sequence database,the DNA sequence homology of 24 strains with F.decemcellulare,F.proliferatum,F.equiseti,F.keratoplasticum,and F.solani were 99%-100%,respectively.
With Sophora japonica Linn.root rot,the pathogens were isolated by tissue separation,tested by the standard Koch's postulation methods,and identified by the methods of microscopy and molecules,and the pathogen species of Chinese scholartree root rot were studied.From the culture characters and morphological characteristics of the 24 strains,the isolated strains were identified as Fusarium sp.These strains of Fusarium could infect in vivo Chinese scholartree barkes and make it black brown and rot.In GenB ank sequence database,the DNA sequence homology of 24 strains with F.decemcellulare,F.proliferatum,F.equiseti,F.keratoplasticum,and F.solani were 99%-100%,respectively.
引文
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[2]SUMMERELL B A,SALLEH B,LESLIE J F.A utilitarian approach to Fusarium identification[J].Plant Disease,2003,87(2):117-128.
[3]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[4]BOOTH C.镰刀菌属[M].陈其煐,译.北京:农业出版社,1988.
[5]高克祥,郑均宝,钟继军.香椿插穗腐烂病病情和病因的研究[J].东北林业大学学报,1995,23(4):47-54.
[6]谯天敏,罗蓉,朱天辉.南方红豆杉根腐病病原及其拮抗芽孢杆菌的鉴定[J].植物保护,2015,41(6):60-66.
[7]刁朝蕾,王艳,刘桂林,等.银杏根腐病病原菌分离及防治药剂筛选[J].林业科技开发,2015,29(3):120-123.
[8]李河,周国英,何末军.油茶根腐病病原菌分子鉴定及其生物学特性研究[J].西南林学院学报,2008,28(5):45-48,56.
[9]袁孟娟,藏香银,韩军,等.丹参根腐病原菌的分离与鉴定[J].仲恺农业工程学院学报,2015,43(2):62-65.
[10]成玉梅,赵丹,康业斌.牡丹根腐病病原的形态与分子鉴定[J].北方园艺,2015(16):116-119.
[11]周亚丽.长春地区蝴蝶兰根腐病病原菌鉴定及化学防治效果研究[D].长春:吉林农业大学,2007.
[12]王中林.金枝槐主要病虫害及其防控技术[J].科学种养,2016(4):32-33.
[13]何永华.刺槐根腐病的研究[J].四川林业科技,1990,11(3):42-45.
[14]曾大鹏.我国杨树病害的研究现状与防治[J].中国森林病虫,2002,21(1):20-26.
[15]董希文,张志强,王丽敏,等.园林绿化树种烂皮病发生规律的研究[J].中国森林病虫,2006,25(4):11-15.