黄鳍金枪鱼头多肽体外抗氧化活性的研究
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摘要
以VC为对照品,测定TIP3C对羟自由基、超氧阴离子自由基、DPPH自由基和ABTS自由基的清除能力,研究黄鳍金枪鱼头多肽TIP3C的体外抗氧化活性。结果表明,TIP3C对4种自由基均具有较强的清除作用,且清除率都随着质量浓度的增大而显著增加(p<0.05),剂量效应关系明显;TIP3C对羟自由基、超氧阴离子自由基、DPPH自由基和ABTS自由基的清除IC_(50)分别为1.724,0.985,2.434和0.242 g/L。TIP3C具备较高的抗氧化能力,为其作为抗氧化肽的开发利用提供理论依据。
Investigate the antioxidant effects of Thunnus albacares head polypeptides(TIP3C) in vitro. The scavenging capacities of TIP3C on OH·, O_2~-·, DPPH· and ABTS· were assayed in contrast with VC. The experimental results indicated that TIP3C had strong effect of scavenging on OH·, O_2~-·, DPPH· and ABTS·. The IC_(50) were 1.724, 0.985, 2.434 and 0.242 g/L, respectively. Moreover, with the increase of the concentration of TIP3C, the scavenging rate of OH·, O_2~-·, DPPH· and ABTS· increased significantly(p<0.05) in a dose-dependent manner. Therefore, TIP3C has strong antioxidant capacity and it provided a theoretical foundation for the development and utilization of antioxidant peptides.
Investigate the antioxidant effects of Thunnus albacares head polypeptides(TIP3C) in vitro. The scavenging capacities of TIP3C on OH·, O_2~-·, DPPH· and ABTS· were assayed in contrast with VC. The experimental results indicated that TIP3C had strong effect of scavenging on OH·, O_2~-·, DPPH· and ABTS·. The IC_(50) were 1.724, 0.985, 2.434 and 0.242 g/L, respectively. Moreover, with the increase of the concentration of TIP3C, the scavenging rate of OH·, O_2~-·, DPPH· and ABTS· increased significantly(p<0.05) in a dose-dependent manner. Therefore, TIP3C has strong antioxidant capacity and it provided a theoretical foundation for the development and utilization of antioxidant peptides.
引文
[1]KIM S Y,JE J Y,KIM S K.Purification and characterization of antioxidant peptide from hoki(Johnius belengerii)frame protein by gastrointestinal digestion[J].Journal of Nutritional Biochemistry,2007,18(1):31-38.
[2]吴晓洒,蔡路昀,曹爱玲,等.水产品加工废弃物中的蛋白及生物活性肽的研究进展[J].食品工业科技,2014,35(23):371-381.
[3]洪鹏志,杨萍,章超桦,等.金枪鱼头的营养成分及其蛋白酶解物的应用[J].食品研究与开发,2007,28(5):140-142.
[4]HERPANDI N H,ROSMA A,NADIAH WAN A W.The tuna fishing industry:A new outlook on fish protein hydrolysates[J].Comprehensive Reviews in Food Science and Food Safety,2011,10(4):195-207.
[5]洪鹏志,杨萍,章超桦,等.黄鳍金枪鱼头酶解蛋白粉营养评价及其应用[J].食品工业科技,2007,28(4):210-212.
[6]蔡本利.黄鳍金枪鱼头蛋白酶解液免疫活性的研究[D].湛江:广东海洋大学,2007.
[7]杨萍,蔡本利,洪鹏志,等.黄鳍金枪鱼头蛋白酶解液及其分级组分对小鼠免疫功能的影响[J].食品与生物技术学报,2009,28(1):132-135.
[8]杨萍,蔡本利,洪鹏志,等.黄鳍金枪鱼头蛋白酶解液及分级组分对小鼠脾淋巴细胞增殖的影响[J].天然产物研究与开发,2010,22(3):498-501.
[9]纪丽娜.金枪鱼头酶解物免疫活性肽的分离及对小鼠腹腔巨噬细胞功能的影响[D].湛江:广东海洋大学,2012.
[10]PRASAD K N,YANG B,SHI J,et al.Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultrahigh-pressure-assisted extraction[J].J Pharmaceutical Biomed Aanaly,2010,51(2):471-477.
[11]JANG M H,KIM H Y,KANG K S,et al.Hydroxyl radical scavenging activities of isoquinoline alkaloids isolated from Coptis chinensis[J].Arch Pharm Res,2009,32(3):341-345.
[12]SFAHLAN A J,MAHMOODZADEH A,HASANZADEH A,et al.Antioxidants and antiradicals in almond hull and shell(Amygdalus communis L.)as a function of genotype[J].Food Chemistry,2009,115(2):529-533.
[13]FLOEGEL A,KIM D O,CHUNG S J,et al.Comparison of ABTS/DPPH assays tomeasure antioxidant capacity in popular antioxidant-rich US foods[J].Journal of Food Composition and Analysis,2011,24(7):1043-1048.
[14]BUTKOVI?V,KLASINC L,BORS W.Kinetic study of flavonoid reactions with stable radicals[J].Journal of Agricultural and Food Chemistry,2004,52(10):2816-2820.
[15]刘微微,任虹,曹学丽,等.天然产物抗氧化活性体外评价方法研究进展[J].食品科学,2010,31(17):415-419.
[16]JIN X C,NING Y.Antioxidant and antitumor activities of the polysaccharide from seed cake of Camellia oleiferaAbel[J].Int J of Biol Macromol,2012(51):364-368.
[17]杨芬,张瑞萍,贺玖明,等.羟自由基的产生、捕集及检测方法[J].药学学报,2007,42(7):692-697.
[18]普燕,敬思群,马泽鑫.4种葡萄抗氧化活性比较[J].食品科学,2013,34(3):109-114.
[19]NIKI E.Assessment of antioxidant capacity in vitro and in vivo[J].Free Radical Biology&Medicine,2010,49(4):503-515.
[20]DURMAZ G.Freeze-dried ABTS method:A ready-to-use radical powder to assess antioxidant capacity of vegetable oils[J].Food Chemistry,2012,133(4):1658-1663.
[21]YANG P,KE H Q,HONG P Z,et al.Antioxidant activity of bigeye tuna(Thunnus obesus)head protein hydrolysate prepared with Alcalase[J].International Journal of Food Science and Technology,2011,46(12):2460-2466.
[22]杨萍,柯虹乔,章超桦,等.大眼金枪鱼头蛋白酶解物1 ku超滤组分的抗氧化活性及其理化性质[J].水产学报,2012,36(8):1297-1303.
[23]任俊凤,任婷婷,朱蓓薇.河豚鱼皮胶原蛋白肽的提取及其抗氧化活性的研究[J].中国食品学报,2009,9(2):77-83.
[24]谭洪亮,郁迪,王斌,等.金枪鱼鱼骨胶原肽的制备及抗氧化活性研究[J].水产学报,2014,38(1):143-148.
[2]吴晓洒,蔡路昀,曹爱玲,等.水产品加工废弃物中的蛋白及生物活性肽的研究进展[J].食品工业科技,2014,35(23):371-381.
[3]洪鹏志,杨萍,章超桦,等.金枪鱼头的营养成分及其蛋白酶解物的应用[J].食品研究与开发,2007,28(5):140-142.
[4]HERPANDI N H,ROSMA A,NADIAH WAN A W.The tuna fishing industry:A new outlook on fish protein hydrolysates[J].Comprehensive Reviews in Food Science and Food Safety,2011,10(4):195-207.
[5]洪鹏志,杨萍,章超桦,等.黄鳍金枪鱼头酶解蛋白粉营养评价及其应用[J].食品工业科技,2007,28(4):210-212.
[6]蔡本利.黄鳍金枪鱼头蛋白酶解液免疫活性的研究[D].湛江:广东海洋大学,2007.
[7]杨萍,蔡本利,洪鹏志,等.黄鳍金枪鱼头蛋白酶解液及其分级组分对小鼠免疫功能的影响[J].食品与生物技术学报,2009,28(1):132-135.
[8]杨萍,蔡本利,洪鹏志,等.黄鳍金枪鱼头蛋白酶解液及分级组分对小鼠脾淋巴细胞增殖的影响[J].天然产物研究与开发,2010,22(3):498-501.
[9]纪丽娜.金枪鱼头酶解物免疫活性肽的分离及对小鼠腹腔巨噬细胞功能的影响[D].湛江:广东海洋大学,2012.
[10]PRASAD K N,YANG B,SHI J,et al.Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultrahigh-pressure-assisted extraction[J].J Pharmaceutical Biomed Aanaly,2010,51(2):471-477.
[11]JANG M H,KIM H Y,KANG K S,et al.Hydroxyl radical scavenging activities of isoquinoline alkaloids isolated from Coptis chinensis[J].Arch Pharm Res,2009,32(3):341-345.
[12]SFAHLAN A J,MAHMOODZADEH A,HASANZADEH A,et al.Antioxidants and antiradicals in almond hull and shell(Amygdalus communis L.)as a function of genotype[J].Food Chemistry,2009,115(2):529-533.
[13]FLOEGEL A,KIM D O,CHUNG S J,et al.Comparison of ABTS/DPPH assays tomeasure antioxidant capacity in popular antioxidant-rich US foods[J].Journal of Food Composition and Analysis,2011,24(7):1043-1048.
[14]BUTKOVI?V,KLASINC L,BORS W.Kinetic study of flavonoid reactions with stable radicals[J].Journal of Agricultural and Food Chemistry,2004,52(10):2816-2820.
[15]刘微微,任虹,曹学丽,等.天然产物抗氧化活性体外评价方法研究进展[J].食品科学,2010,31(17):415-419.
[16]JIN X C,NING Y.Antioxidant and antitumor activities of the polysaccharide from seed cake of Camellia oleiferaAbel[J].Int J of Biol Macromol,2012(51):364-368.
[17]杨芬,张瑞萍,贺玖明,等.羟自由基的产生、捕集及检测方法[J].药学学报,2007,42(7):692-697.
[18]普燕,敬思群,马泽鑫.4种葡萄抗氧化活性比较[J].食品科学,2013,34(3):109-114.
[19]NIKI E.Assessment of antioxidant capacity in vitro and in vivo[J].Free Radical Biology&Medicine,2010,49(4):503-515.
[20]DURMAZ G.Freeze-dried ABTS method:A ready-to-use radical powder to assess antioxidant capacity of vegetable oils[J].Food Chemistry,2012,133(4):1658-1663.
[21]YANG P,KE H Q,HONG P Z,et al.Antioxidant activity of bigeye tuna(Thunnus obesus)head protein hydrolysate prepared with Alcalase[J].International Journal of Food Science and Technology,2011,46(12):2460-2466.
[22]杨萍,柯虹乔,章超桦,等.大眼金枪鱼头蛋白酶解物1 ku超滤组分的抗氧化活性及其理化性质[J].水产学报,2012,36(8):1297-1303.
[23]任俊凤,任婷婷,朱蓓薇.河豚鱼皮胶原蛋白肽的提取及其抗氧化活性的研究[J].中国食品学报,2009,9(2):77-83.
[24]谭洪亮,郁迪,王斌,等.金枪鱼鱼骨胶原肽的制备及抗氧化活性研究[J].水产学报,2014,38(1):143-148.