铁苋菜不同极性萃取物的抗氧化及抑菌活性研究
|
摘要
采用1,1-二苯基-2-三硝基苯肼(DPPH)法、β-胡萝卜素漂白法和清除一氧化氮自由基(NO)法来测定不同极性铁苋菜萃取物的抗氧化活性;通过微孔比浊法比较铁苋菜萃取物对3种革兰氏阴性细菌(大肠杆菌、铜绿假单胞菌和幽门螺杆菌)和4种革兰氏阳性细菌(表皮葡萄球菌、枯草芽孢杆菌、藤黄微球菌和金黄色葡萄球菌)的抑菌效果。结果表明,铁苋菜不同极性萃取物均有较好的清除DPPH、NO自由基和抑制β-胡萝卜素漂白的效果,浓度为2.0mg/mL时,正丁醇萃取物清除DPPH自由基和抑制β-胡萝卜素漂白能力最佳,DPPH自由基清除率为94.60%,β-胡萝卜素抑制率为83.15%,乙酸乙酯萃取物清除NO自由基能力最强,清除率为82.72%。抑菌试验结果显示,铁苋菜各萃取物均有较好抑菌效果,对革兰氏阳性菌抑菌效果优于革兰氏阴性菌,其中石油醚萃取物对大肠杆菌、表皮葡萄球菌和金黄色葡萄球菌抑制作用最佳,MIC值分别为0.625,0.315,1.250mg/mL。铁苋菜萃取物具有较好的抗氧化及抑菌效果。
1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)method,β-carotene bleaching method and nitric oxide(NO)method were used to determine the antioxidant activities of different extracts from Acalypha australis L.;All the extracts were evaluated for their bacteriostatic effect using microporous turbidimetry method,and three gram-negative bacteria:Escherichia coli,Pseudomonas aeruginosa,Helicobacter pylori and four grampositive bacteria:Staphylococcus epidermidis,Bacillus subtilis,Micrococcus luteus,Staphylococcus aureus were determined in our research.The results showed all the extracts we determined had the activities of better scavenging DPPH,NO free radicals and inhibition ofβ-carotene bleaching.At the concentration was 2.0 mg/mL,the n-butanol extract had the strongest power to scavenge DPPH free radicals and inhibitβ-carotene oxidation activity.The DPPH free radical scavenging rate was94.60% and theβ-carotene inhibition rate was 83.15%.Furthermore,the ethyl acetate extract had the strongest ability to scavenge NO radicals,and the clearance rate was 82.72%.In addition,the results of bacteriostatic experiments showed that all the extracts had good antibacterial effect,and its inhibitory effect against Gram-positive bacteria was higher than that of Gram-negative bacteria.Especially,the petroleum ether extract had the best inhibitory effect on Escherichia coli,Staphylococcus epidermidis and Staphylococcus aureus,and the minimum inhibitory concentration(MIC)values were 0.625,0.315 and1.250 mg/mL,respectively.In conclusion,the extracts of Acalypha australis L.have better anti-oxidation and antibacterial effects and this research was profitable for the further study of the separation of avtive compounds from Acalypha australis L..
1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)method,β-carotene bleaching method and nitric oxide(NO)method were used to determine the antioxidant activities of different extracts from Acalypha australis L.;All the extracts were evaluated for their bacteriostatic effect using microporous turbidimetry method,and three gram-negative bacteria:Escherichia coli,Pseudomonas aeruginosa,Helicobacter pylori and four grampositive bacteria:Staphylococcus epidermidis,Bacillus subtilis,Micrococcus luteus,Staphylococcus aureus were determined in our research.The results showed all the extracts we determined had the activities of better scavenging DPPH,NO free radicals and inhibition ofβ-carotene bleaching.At the concentration was 2.0 mg/mL,the n-butanol extract had the strongest power to scavenge DPPH free radicals and inhibitβ-carotene oxidation activity.The DPPH free radical scavenging rate was94.60% and theβ-carotene inhibition rate was 83.15%.Furthermore,the ethyl acetate extract had the strongest ability to scavenge NO radicals,and the clearance rate was 82.72%.In addition,the results of bacteriostatic experiments showed that all the extracts had good antibacterial effect,and its inhibitory effect against Gram-positive bacteria was higher than that of Gram-negative bacteria.Especially,the petroleum ether extract had the best inhibitory effect on Escherichia coli,Staphylococcus epidermidis and Staphylococcus aureus,and the minimum inhibitory concentration(MIC)values were 0.625,0.315 and1.250 mg/mL,respectively.In conclusion,the extracts of Acalypha australis L.have better anti-oxidation and antibacterial effects and this research was profitable for the further study of the separation of avtive compounds from Acalypha australis L..
引文
[1]梁建丽,韦丽富,周婷婷,等.铁苋菜有效成分及药理作用研究概况[J].亚太传统医药,2015,11(3):45-47.
[2]SEEBALUCK R,GURIB-FAKIM A,MAHOMOODALLYF.Medicinal plants from the genus Acalypha(Euphorbiaceae):A review of their ethnopharmacology and phytochemistry[J].Journal of Ethnopharmacology,2015,159:137-157.
[3]江苏新医学院.中药大辞典:下册[M].上海:上海科学技术出版社,2002:3 809-3 811.
[4]张小琼.裂苞铁苋菜和紫芫花的化学成分与活性研究[D].武汉:华中科技大学,2010:2-4.
[5]王晓岚,郁开北,彭树林.铁苋菜地上部分的化学成分研究[J].中国中药杂志,2008,33(12):1 415-1 417.
[6]邓莉.铁苋菜抗溃疡性结肠炎的药效、机制及药效物质基础研究[D].上海:第二军医大学,2005:50-52.
[7]魏秀娟,向发椿,崔明筠,等.铁苋菜多糖体外抗氧化研究[J].中国实验方剂学杂志,2013,19(3):197-200.
[8]崔明筠.铁苋菜总黄酮的提取纯化及其抗氧化活性的研究[D].绵阳:西南科技大学,2014:49-51.
[9]梁曾恩妮,蒋道松,刘作梅,等.铁苋菜总黄酮提取工艺优化及其抑菌效果的初步鉴定[J].湖南农业科学,2008(2):110-112.
[10]王春景,胡小梅,刘高峰,等.铁苋菜不同提取物的抗氧化性及抑菌活性[J].光谱实验室,2012,29(3):1 812-1 816.
[11]SINGLETON V L,ORTHOFER R,LAMUELA-RAVENTS R M.Analysis of total phenols and other oxidation substrates and antioxidants by means of folinciocalteu reagent[J].Methods in Enzymology,1999,299C(1):152-178.
[12]游见明,曹新志.福林酚法测定茶树中茶多酚的分布水平[J].湖北农业科学,2013,52(10):2 417-2 419.
[13]杜丹丹,李建科.Folin-Ciocalteu比色法测定石榴皮多酚含量条件的优化[J].西北农林科技大学学报:自然科学版,2011(5):190-196.
[14]任秋蓉,王亚男,王玥,等.密花香薷总黄酮体外抗氧化及抗肿瘤活性研究[J].天然产物研究与开发,2017,29(1):14-21.
[15]詹济华,孟英才,谭洋,等.铁苋菜乙醇提取物抗流感活性及其机理的初步研究[J].天然产物研究与开发,2016,28(11):1 706-1 711,1 751.
[16]胡丽娟,刘蔚,吴欢欢,等.加味小柴胡汤抗幽门螺杆菌的有效物质部位群研究[J].中国现代中药,2016,18(3):307-311.
[17]张恩户,乔慧,王光建,等.微孔比浊法测定牛黄上清片的抗菌效价[J].中药药理与临床,2012,28(3):23-25.
[18]黄文平,吴柳瑾,殷文静,等.铁苋菜中原儿茶酸和原儿茶醛含量的高效液相色谱法测定[J].时珍国医国药,2016(7):1 570-1 572.
[19]魏学军,林先燕,李雪营,等.不同采收期和部位的黔产铁苋菜中没食子酸和总黄酮的含量[J].华西药学杂志,2014,29(5):580-582.
[20]景书灏.铁苋菜、白三叶草的化学成分及抗菌活性研究[D].重庆:重庆大学,2010:10.
[2]SEEBALUCK R,GURIB-FAKIM A,MAHOMOODALLYF.Medicinal plants from the genus Acalypha(Euphorbiaceae):A review of their ethnopharmacology and phytochemistry[J].Journal of Ethnopharmacology,2015,159:137-157.
[3]江苏新医学院.中药大辞典:下册[M].上海:上海科学技术出版社,2002:3 809-3 811.
[4]张小琼.裂苞铁苋菜和紫芫花的化学成分与活性研究[D].武汉:华中科技大学,2010:2-4.
[5]王晓岚,郁开北,彭树林.铁苋菜地上部分的化学成分研究[J].中国中药杂志,2008,33(12):1 415-1 417.
[6]邓莉.铁苋菜抗溃疡性结肠炎的药效、机制及药效物质基础研究[D].上海:第二军医大学,2005:50-52.
[7]魏秀娟,向发椿,崔明筠,等.铁苋菜多糖体外抗氧化研究[J].中国实验方剂学杂志,2013,19(3):197-200.
[8]崔明筠.铁苋菜总黄酮的提取纯化及其抗氧化活性的研究[D].绵阳:西南科技大学,2014:49-51.
[9]梁曾恩妮,蒋道松,刘作梅,等.铁苋菜总黄酮提取工艺优化及其抑菌效果的初步鉴定[J].湖南农业科学,2008(2):110-112.
[10]王春景,胡小梅,刘高峰,等.铁苋菜不同提取物的抗氧化性及抑菌活性[J].光谱实验室,2012,29(3):1 812-1 816.
[11]SINGLETON V L,ORTHOFER R,LAMUELA-RAVENTS R M.Analysis of total phenols and other oxidation substrates and antioxidants by means of folinciocalteu reagent[J].Methods in Enzymology,1999,299C(1):152-178.
[12]游见明,曹新志.福林酚法测定茶树中茶多酚的分布水平[J].湖北农业科学,2013,52(10):2 417-2 419.
[13]杜丹丹,李建科.Folin-Ciocalteu比色法测定石榴皮多酚含量条件的优化[J].西北农林科技大学学报:自然科学版,2011(5):190-196.
[14]任秋蓉,王亚男,王玥,等.密花香薷总黄酮体外抗氧化及抗肿瘤活性研究[J].天然产物研究与开发,2017,29(1):14-21.
[15]詹济华,孟英才,谭洋,等.铁苋菜乙醇提取物抗流感活性及其机理的初步研究[J].天然产物研究与开发,2016,28(11):1 706-1 711,1 751.
[16]胡丽娟,刘蔚,吴欢欢,等.加味小柴胡汤抗幽门螺杆菌的有效物质部位群研究[J].中国现代中药,2016,18(3):307-311.
[17]张恩户,乔慧,王光建,等.微孔比浊法测定牛黄上清片的抗菌效价[J].中药药理与临床,2012,28(3):23-25.
[18]黄文平,吴柳瑾,殷文静,等.铁苋菜中原儿茶酸和原儿茶醛含量的高效液相色谱法测定[J].时珍国医国药,2016(7):1 570-1 572.
[19]魏学军,林先燕,李雪营,等.不同采收期和部位的黔产铁苋菜中没食子酸和总黄酮的含量[J].华西药学杂志,2014,29(5):580-582.
[20]景书灏.铁苋菜、白三叶草的化学成分及抗菌活性研究[D].重庆:重庆大学,2010:10.