干旱胁迫下拟南芥中H_2S与ABA信号关系研究
|
摘要
以野生型拟南芥(WT)、硫化氢(H_2S)合成酶缺失型突变体lcd、脱落酸(ABA)合成缺失型突变体aba1实生苗为材料,以0.3 mol·L~(-1)甘露醇模拟干旱胁迫,研究干旱胁迫对ABA含量、H_2S含量的影响,及其在拟南芥抵抗干旱胁迫中的作用及信号关系。结果显示:干旱胁迫显著提高LCD和ABA1基因相对表达以及H_2S含量,ABA含量;干旱胁迫显著抑制突变体lcd、aba1的种子萌发;干旱胁迫下,外施NaHS促进干旱胁迫下WT、lcd和aba1中內源H_2S的产生及上调LCD、ABA1基因相对表达,而外施ABA提高干旱胁迫下WT、aba1中H_2S含量及LCD、ABA1基因相对表达,但是对lcd中H_2S含量及LCD基因相对表达没有显著影响。研究结果表明,信号分子H_2S和ABA在拟南芥的干旱胁迫响应中发挥一定的作用,且H_2S位于ABA的下游参与调控拟南芥的信号过程。
With the seedlings of wild type Arabidopsis thaliana(WT),hydrogen sulfide(H_2S) synthase deletion mutant lcd,abscisic acid(ABA) deletion mutant aba1,0.3 mol · L~(-1) mannitol simulated drought stress,we studied the effects of drought stress on ABA content and H_2S content,analyzed their roles and signal relationships in drought tolerance.Drought stress significantly increased the LCD and ABA1 gene relative expressions,contents of H_2S and ABA;Drought stress significantly inhibited the seed germination of lcd and aba1 mutants;Application of NaHS and ABA significantly increased the endogenous H_2S content in WT under drought stress;Application of NaHS could promote the endogenous H_2S production in WT,lcd and aba1,the relative expression of LCD and ABA1 also up-regulated under drought stress.While the application of ABA increased the H_2S content and the LCD,ABA1 relative expressions in WT and aba1 under drought stress,but had no significant effect on the H_2S content and LCD relative expression in lcd.The results showed that the signal molecules H_2S and ABA played a vital role response to the drought stress in Arabidopsis,and H_2S is located at downstream of ABA to participated in the regulation of signaling process.
With the seedlings of wild type Arabidopsis thaliana(WT),hydrogen sulfide(H_2S) synthase deletion mutant lcd,abscisic acid(ABA) deletion mutant aba1,0.3 mol · L~(-1) mannitol simulated drought stress,we studied the effects of drought stress on ABA content and H_2S content,analyzed their roles and signal relationships in drought tolerance.Drought stress significantly increased the LCD and ABA1 gene relative expressions,contents of H_2S and ABA;Drought stress significantly inhibited the seed germination of lcd and aba1 mutants;Application of NaHS and ABA significantly increased the endogenous H_2S content in WT under drought stress;Application of NaHS could promote the endogenous H_2S production in WT,lcd and aba1,the relative expression of LCD and ABA1 also up-regulated under drought stress.While the application of ABA increased the H_2S content and the LCD,ABA1 relative expressions in WT and aba1 under drought stress,but had no significant effect on the H_2S content and LCD relative expression in lcd.The results showed that the signal molecules H_2S and ABA played a vital role response to the drought stress in Arabidopsis,and H_2S is located at downstream of ABA to participated in the regulation of signaling process.
引文
1.Hellal F A,El-Shabrawi H M,El-Hady M A,et al.Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars[J].Journal of Genetic Engineering and Biotechnology,2018,16(1):203-212.
2.Blum A.Osmotic adjustment is a prime drought stress adaptive engine in support of plant production[J].Plant Cell&Environment,2017,40(1):4-10.
3.Wang R.Hydrogen sulfide:a new EDRF[J].Kidney International,2009,76(7):700-704.
4.Huang P,Shen Z Z,Yu W,et al.Hydrogen sulfide inhibits high-salt diet-induced myocardial oxidative stress and myocardial hypertrophy in Dahl rats[J].Frontiers in Pharmacology,2017,8:128.
5.Wang R.Two's company,three's a crowd:can H2S be the third endogenous gaseous transmitter[J].FASEB Journal,2002,16(13):1792-1798.
6.Baudouin E,Poilevey A,Hewage N I,et al.The significance of hydrogen sulfide for Arabidopsis seed germination[J].Frontiers in Plant Science,2016,7:930.
7.Olas B.Hydrogen sulfide in signaling pathways[J].Clinica Chimica Acta,2015,439:212-218.
8.Zhang H,Hu L Y,Hu K D,et al.Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress[J].Journal of Integrative Plant Biology,2008,50(12):1518-1529.
9.Zhang H,Tang J,Liu X P,et al.Hydrogen sulfide promotes root organogenesis in Ipomoea batatas,Salix matsudana and Glycine max[J].Journal of Integrative Plant Biology,2009,51(12):1086-1094.
10.Jin Z P,Shen J J,Qiao Z J,et al.Hydrogen sulfide improves drought resistance in Arabidopsis thaliana[J].Biochemical and Biophysical Research Communications,2011,414(3):481-486.
11.Fang H H,Liu Z Q,Jin Z P,et al.An emphasis of hydrogen sulfide-cysteine cycle on enhancing the tolerance to chromium stress in Arabidopsis[J].Environmental Pollution,2016,213:870-877.
12.朱晓琛,张汉马,南文斌.脱落酸调控植物根系生长发育的研究进展[J].植物生理学报,2017,53(7):1123-1130.Zhu X C,Zhang H M,Nan W B.Research progress on regulation of ABA in plant root development[J].Plant Physiology Journal,2017,53(7):1123-1130.
13.Espasandin F D,Maiale S J,Calzadilla P,et al.Transcriptional regulation of 9-cis-epoxycarotenoid dioxygenase(NCED)gene by putrescine accumulation positively modulates ABA synthesis and drought tolerance in Lotus tenuis plants[J].Plant Physiology and Biochemistry,2014,76:29-35.
14.Zhang J H,Jia W S,Yang J C,et al.Role of ABA in integrating plant responses to drought and salt stresses[J].Field Crops Research,2006,97(1):111-119.
15.董寰,白玲,宋纯鹏.植物激素ABA调控侧根生长发育的研究进展[J].中国农学通报,2016,32(18):76-82.Dong H,Bai L,Song C P.Growth and development of lateral root regulated by plant hormone ABA[J].Chinese Agricultural Science Bulletin,2016,32(18):76-82.
16.Li C,Tan D X,Liang D,et al.Melatonin mediates the regulation of ABA metabolism,free-radical scavenging,and stomatal behaviour in two Malus species under drought stress[J].Journal of Experimental Botany,2015,66(3):669-680.
17.金竹萍.硫化氢对拟南芥应答干旱胁迫的信号作用机制[D].太原:山西大学,2013.Jin Z P.Signal effects and mechanisms of hydrogen sulfide in response to drought stress in Arabidopsis[D].Taiyuan:Shanxi University,2013.
18.Zhong G L,Lin R X,Xiao J L.Hydrogen sulfide acts as a downstream signal molecule in salicylic acid-induced heat tolerance in maize(Zea mays L.)seedlings[J].Journal of Plant Physiology,2015,177:121-127.
19.Chandra D,Srivastava R,Glick B R,et al.Drought-tolerant Pseudomonas spp.improve the growth performance of finger millet(Eleusine coracana(L.)Gaertn.)under nonstressed and drought-stressed conditions[J].Pedosphere,2018,28(2):227-240.
20.Li L,Rose P,Moore P K.Hydrogen sulfide and cell signaling[J].Annual Review of Pharmacology and Toxicology,2011,51(1):169-187.
21.Da Silva C J,Fontes E P B,Modolo L V.Salinity-induced accumulation of endogenous H2S and NO is associated with modulation of the antioxidant and redox defense systems in Nicotiana tabacum L.cv.Havana[J].Plant Science,2017,256:148-159.
22.Chen H,Zhang J,Neff M M,et al.Integration of light and abscisic acid signaling during seed germination and early seedling development[J].Proceedings of the National A-cademy of Sciences of the United States of America,2008,105(11):4495-4500.
23.孙丽敏,裴雁曦,刘志强.H2S信号和WRKY在ABA调节根生长和气孔运动中的关系[J].植物研究,2016,36(1):97-104.Sun L M,Pei Y X,Liu Z Q.Relationship between H2S signal and WRKY in ABA regulating root growth and stomatal movement[J].Bulletin of Botanical Research,2016,36(1):97-104.
24.赵敏,杨宁,陈璐,等.拟南芥中H2S与PLDα1响应干旱胁迫的作用研究[J].植物研究,2018,38(3):406-414.Zhao M,Yang N,Chen L,et al.Effects of H2S and PLDα1on Drought Stress in Arabidopsis thaliana[J].Bulletin of Botanical Research,2018,38(3):406-414.
25.Qiao Z J,Jing T,Jin Z P,et al.CDPKs enhance Cd tolerance through intensifying H2S signal in Arabidopsis thaliana[J].Plant and Soil,2016,398(1-2):99-110.
2.Blum A.Osmotic adjustment is a prime drought stress adaptive engine in support of plant production[J].Plant Cell&Environment,2017,40(1):4-10.
3.Wang R.Hydrogen sulfide:a new EDRF[J].Kidney International,2009,76(7):700-704.
4.Huang P,Shen Z Z,Yu W,et al.Hydrogen sulfide inhibits high-salt diet-induced myocardial oxidative stress and myocardial hypertrophy in Dahl rats[J].Frontiers in Pharmacology,2017,8:128.
5.Wang R.Two's company,three's a crowd:can H2S be the third endogenous gaseous transmitter[J].FASEB Journal,2002,16(13):1792-1798.
6.Baudouin E,Poilevey A,Hewage N I,et al.The significance of hydrogen sulfide for Arabidopsis seed germination[J].Frontiers in Plant Science,2016,7:930.
7.Olas B.Hydrogen sulfide in signaling pathways[J].Clinica Chimica Acta,2015,439:212-218.
8.Zhang H,Hu L Y,Hu K D,et al.Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress[J].Journal of Integrative Plant Biology,2008,50(12):1518-1529.
9.Zhang H,Tang J,Liu X P,et al.Hydrogen sulfide promotes root organogenesis in Ipomoea batatas,Salix matsudana and Glycine max[J].Journal of Integrative Plant Biology,2009,51(12):1086-1094.
10.Jin Z P,Shen J J,Qiao Z J,et al.Hydrogen sulfide improves drought resistance in Arabidopsis thaliana[J].Biochemical and Biophysical Research Communications,2011,414(3):481-486.
11.Fang H H,Liu Z Q,Jin Z P,et al.An emphasis of hydrogen sulfide-cysteine cycle on enhancing the tolerance to chromium stress in Arabidopsis[J].Environmental Pollution,2016,213:870-877.
12.朱晓琛,张汉马,南文斌.脱落酸调控植物根系生长发育的研究进展[J].植物生理学报,2017,53(7):1123-1130.Zhu X C,Zhang H M,Nan W B.Research progress on regulation of ABA in plant root development[J].Plant Physiology Journal,2017,53(7):1123-1130.
13.Espasandin F D,Maiale S J,Calzadilla P,et al.Transcriptional regulation of 9-cis-epoxycarotenoid dioxygenase(NCED)gene by putrescine accumulation positively modulates ABA synthesis and drought tolerance in Lotus tenuis plants[J].Plant Physiology and Biochemistry,2014,76:29-35.
14.Zhang J H,Jia W S,Yang J C,et al.Role of ABA in integrating plant responses to drought and salt stresses[J].Field Crops Research,2006,97(1):111-119.
15.董寰,白玲,宋纯鹏.植物激素ABA调控侧根生长发育的研究进展[J].中国农学通报,2016,32(18):76-82.Dong H,Bai L,Song C P.Growth and development of lateral root regulated by plant hormone ABA[J].Chinese Agricultural Science Bulletin,2016,32(18):76-82.
16.Li C,Tan D X,Liang D,et al.Melatonin mediates the regulation of ABA metabolism,free-radical scavenging,and stomatal behaviour in two Malus species under drought stress[J].Journal of Experimental Botany,2015,66(3):669-680.
17.金竹萍.硫化氢对拟南芥应答干旱胁迫的信号作用机制[D].太原:山西大学,2013.Jin Z P.Signal effects and mechanisms of hydrogen sulfide in response to drought stress in Arabidopsis[D].Taiyuan:Shanxi University,2013.
18.Zhong G L,Lin R X,Xiao J L.Hydrogen sulfide acts as a downstream signal molecule in salicylic acid-induced heat tolerance in maize(Zea mays L.)seedlings[J].Journal of Plant Physiology,2015,177:121-127.
19.Chandra D,Srivastava R,Glick B R,et al.Drought-tolerant Pseudomonas spp.improve the growth performance of finger millet(Eleusine coracana(L.)Gaertn.)under nonstressed and drought-stressed conditions[J].Pedosphere,2018,28(2):227-240.
20.Li L,Rose P,Moore P K.Hydrogen sulfide and cell signaling[J].Annual Review of Pharmacology and Toxicology,2011,51(1):169-187.
21.Da Silva C J,Fontes E P B,Modolo L V.Salinity-induced accumulation of endogenous H2S and NO is associated with modulation of the antioxidant and redox defense systems in Nicotiana tabacum L.cv.Havana[J].Plant Science,2017,256:148-159.
22.Chen H,Zhang J,Neff M M,et al.Integration of light and abscisic acid signaling during seed germination and early seedling development[J].Proceedings of the National A-cademy of Sciences of the United States of America,2008,105(11):4495-4500.
23.孙丽敏,裴雁曦,刘志强.H2S信号和WRKY在ABA调节根生长和气孔运动中的关系[J].植物研究,2016,36(1):97-104.Sun L M,Pei Y X,Liu Z Q.Relationship between H2S signal and WRKY in ABA regulating root growth and stomatal movement[J].Bulletin of Botanical Research,2016,36(1):97-104.
24.赵敏,杨宁,陈璐,等.拟南芥中H2S与PLDα1响应干旱胁迫的作用研究[J].植物研究,2018,38(3):406-414.Zhao M,Yang N,Chen L,et al.Effects of H2S and PLDα1on Drought Stress in Arabidopsis thaliana[J].Bulletin of Botanical Research,2018,38(3):406-414.
25.Qiao Z J,Jing T,Jin Z P,et al.CDPKs enhance Cd tolerance through intensifying H2S signal in Arabidopsis thaliana[J].Plant and Soil,2016,398(1-2):99-110.