羌活与宽叶羌活的GC/HPLC指纹图谱研究
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摘要
目的:分别建立羌活与宽叶羌活的GC指纹图谱、HPLC指纹图谱,为羌活的挥发性及非挥发性成分质量评价与控制提供有效可靠方法。方法:采用GC、HPLC色谱法分别建立羌活与宽叶羌活的指纹图谱,通过相似度评价软件,综合GC、HPLC指纹图谱相似度结果对222批样品的挥发性及非挥发性成分进行质量分析。结果:建立的GC指纹图谱结果显示,羌活共有峰有10个,宽叶羌活共有峰有12个,二者共有成分有9个。HPLC指纹图谱结果显示,羌活共有峰有9个,宽叶羌活共有峰有7个,二者共有成分6个。其中,羌活醇与异欧前胡素在羌活和宽叶羌活中的含量存在明显差异。GC和HPLC指纹图谱结果均显示,222批样品中有7批伪品,有21批宽叶羌活样品,有118批羌活样品,有76批含有两种基原羌活的样品,二者结果一致。结论:建立的气相及液相指纹图谱能有效区分羌活的基原与真伪,并能够有效表征药材的质量优劣,可为羌活的整体质量评价及控制提供依据。
Objective: To establish a GC/HPLC fingerprint of Notopterygium inchum Ting ex H, T-Chang and Notopterygium franchetii H. de Boiss, and provide an effective and reliable method for the quality evaluation and quality control of volatile and non-volatile components of Notopterygium inchum Ting ex H, T-Chang. Methods:The fingerprints of Notopterygium inchum Ting ex H, T-Chang and Notopterygium franchetii H. de Boiss were established by GC and HPLC. The similarity evaluation software was combined with GC and HPLC fingerprint analysis results to analyze the overall quality of the volatile and non-volatile components of 222 batches. Results:The results of GC fingerprinting showed that there were 10 common peaks in Notopterygium inchum Ting ex H,T-Chang, 12 common peaks in Notopterygium franchetii H. de Boiss, and 9 common components in both. HPLC fingerprint results showed that there were 9 common peaks in Notopterygium inchum Ting ex H, T-Chang, 7 common peaks in Notopterygium franchetii H. de Boiss, and 6 common components in both. Among them, there were significant differences in the content of in Notopterygium inchum and Notopterygium franchetii. The results of GC and HPLC fingerprints showed that there were 7 batches of fake products in the 222 batches, 21 batches of Notopterygium franchetii H. de Boiss, 118 batches of Notopterygium inchum Ting ex H, T-Chang, and 76 batches containing two kinds of bases. The results were same. Conclusion: The established GC fingerprints and HPLC fingerprints can effectively distinguish the base resource and authenticity, and can effectively characterize the quality of medicinal materials, which can provide a basis for the overall quality evaluation and control of Notopterygium.
Objective: To establish a GC/HPLC fingerprint of Notopterygium inchum Ting ex H, T-Chang and Notopterygium franchetii H. de Boiss, and provide an effective and reliable method for the quality evaluation and quality control of volatile and non-volatile components of Notopterygium inchum Ting ex H, T-Chang. Methods:The fingerprints of Notopterygium inchum Ting ex H, T-Chang and Notopterygium franchetii H. de Boiss were established by GC and HPLC. The similarity evaluation software was combined with GC and HPLC fingerprint analysis results to analyze the overall quality of the volatile and non-volatile components of 222 batches. Results:The results of GC fingerprinting showed that there were 10 common peaks in Notopterygium inchum Ting ex H,T-Chang, 12 common peaks in Notopterygium franchetii H. de Boiss, and 9 common components in both. HPLC fingerprint results showed that there were 9 common peaks in Notopterygium inchum Ting ex H, T-Chang, 7 common peaks in Notopterygium franchetii H. de Boiss, and 6 common components in both. Among them, there were significant differences in the content of in Notopterygium inchum and Notopterygium franchetii. The results of GC and HPLC fingerprints showed that there were 7 batches of fake products in the 222 batches, 21 batches of Notopterygium franchetii H. de Boiss, 118 batches of Notopterygium inchum Ting ex H, T-Chang, and 76 batches containing two kinds of bases. The results were same. Conclusion: The established GC fingerprints and HPLC fingerprints can effectively distinguish the base resource and authenticity, and can effectively characterize the quality of medicinal materials, which can provide a basis for the overall quality evaluation and control of Notopterygium.
引文
[1]中国药典:一部[S].2015:182.
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[4]李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[5]ZHANG Ya-jun,XIE Fang,ZHANG Yu,et al.Research Progress on Chemical Components of Notopterygium[J].Hunan Agricultural Sciences(湖南农业科学),2017,(4):124-126.
[6]徐国均,徐珞珊,王峥涛.常用中药材品种整理与质量研究(第三册)[M].福建科学技术出版社,1999:208-213.
[7]乔荣荣,严国俊,田荣,等.羌活挥发性成分的气相色谱/质谱分析[J].海峡药学,2017,29(4):52-56.
[2]JIANG Shun-yuan,SUN Hui,WANG Hong-lan,et al.Industrialization Condition and Development Strategy of Notopterygii Rhizoma et Radix[J].Chinese Journal of Chinese Materia Medica(中国中药杂志),2017,42(14):2627-2632.
[3]LIU Dong-fang,ZHAO Li-na,LI Yin-feng,et al.Research Progress and Application in Fingerprint Technology on Chinese Materia Medica[J].Chinese Traditional and Herbal Drugs(中草药),2016,47(22):4085-4094.
[4]李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[5]ZHANG Ya-jun,XIE Fang,ZHANG Yu,et al.Research Progress on Chemical Components of Notopterygium[J].Hunan Agricultural Sciences(湖南农业科学),2017,(4):124-126.
[6]徐国均,徐珞珊,王峥涛.常用中药材品种整理与质量研究(第三册)[M].福建科学技术出版社,1999:208-213.
[7]乔荣荣,严国俊,田荣,等.羌活挥发性成分的气相色谱/质谱分析[J].海峡药学,2017,29(4):52-56.