HPLC法同时测定藏药双花千里光中6个化学成分的含量
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摘要
目的:建立同时测定藏药双花千里光中的绿原酸、芦丁、金丝桃苷、异槲皮苷、烟花苷和紫云英苷6个化学成分含量的HPLC方法。方法:双花千里光药材用70%乙醇-水提取;以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱(0～2 min,0→16%A;2～6 min,16%A→15%A;6～8 min,15%A;8～13 min,15%A→20%A;13～16 min,20%A→25%A;16～19 min,25%A→20%A;19～26 min,20%A→30%A),检测波长为360 nm。结果:所测6个化学成分在所取质量浓度范围内的线性关系均良好,r值都大于0.999 0;精密度、重复性和稳定性良好;平均回收率为96.1%～99.4%,RSD为0.84%～1.8%;6个化学成分绿原酸、芦丁、金丝桃苷、异槲皮苷、烟花苷、紫云英苷的含量测定结果分别为1.75%、1.26%、0.86%、1.20%、0.57%、1.29%。结论:建立的HPLC方法可用于同时测定双花千里光药材中6个化学成分,可为藏药双花千里光药材的质量控制提供一定的参考。
Objective:To establish an HPLC method for simultaneous determination of six chemical components including chlorogenic acid,rutin,hyperoside,isoquercitrin,kaempferol-3-O-rutinoside,and astragalin in Tibetan medicine Senecio dianthus Franch..Methods:The Senecio dianthus Franch.herbal medicine was extracted with70% ethanol-water.The mobile phase was acetonitrile(A)-0.1% aqueous formic acid(B)in gradient elution(0-2 min,0 → 16%A;2-6 min,16%A → 15%A;6-8 min,15%A;8-13 min,15%A → 20%A;13-16 min,20%A → 25%A;16-19 min,25%A → 20%A;19-26 min,20%A → 30%A).The detection wavelength was 360 nm.Results:The linear relationship of the six chemical components was good,and the r values were all above 0.999 0.Results of precision,repeatability and stability tests were good.The average recovery were 96.1%-99.4%with RSDs of 0.84%-1.8%.The contents of the six chemical components were 1.75%(chlorogenicacid),1.26%(rutin),0.86%(hyperoside),1.20%(isoquercitrin),0.57%(kaempferol-3-O-rutinoside),and 1.29%(astragalin).Conclusion:The established HPLC method can be used to simultaneously determine six chemical components in Senecio dianthus Franch..It can provide reference for the quality control of Tibetan medicine Senecio dianthus Franch..
Objective:To establish an HPLC method for simultaneous determination of six chemical components including chlorogenic acid,rutin,hyperoside,isoquercitrin,kaempferol-3-O-rutinoside,and astragalin in Tibetan medicine Senecio dianthus Franch..Methods:The Senecio dianthus Franch.herbal medicine was extracted with70% ethanol-water.The mobile phase was acetonitrile(A)-0.1% aqueous formic acid(B)in gradient elution(0-2 min,0 → 16%A;2-6 min,16%A → 15%A;6-8 min,15%A;8-13 min,15%A → 20%A;13-16 min,20%A → 25%A;16-19 min,25%A → 20%A;19-26 min,20%A → 30%A).The detection wavelength was 360 nm.Results:The linear relationship of the six chemical components was good,and the r values were all above 0.999 0.Results of precision,repeatability and stability tests were good.The average recovery were 96.1%-99.4%with RSDs of 0.84%-1.8%.The contents of the six chemical components were 1.75%(chlorogenicacid),1.26%(rutin),0.86%(hyperoside),1.20%(isoquercitrin),0.57%(kaempferol-3-O-rutinoside),and 1.29%(astragalin).Conclusion:The established HPLC method can be used to simultaneously determine six chemical components in Senecio dianthus Franch..It can provide reference for the quality control of Tibetan medicine Senecio dianthus Franch..
引文
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[2]青海省药品检验所,青海省藏医药研究所.中国藏药.第三卷[M].上海:上海科学技术出版社,1996:280Qinghai Provincial Institute of Drug Control,Qinghai Institute of Tibetan Medicine.Chinese Tibetan Medicine.VolumeⅢ[M].Shanghai:Shanghai Scientific&Technical Publishers,1996:280
[3]王晓玲,梁思琦,李文君,等.藏药双花千里光中绿原酸的分离纯化及结构鉴定[J].西南民族大学学报(自然科学版),2016,42(1):52WANG XL,LIANG SQ,LI WJ,et al.Isolation and structure identification of chlorogenic acid from Senecio dianthus Franch.[J].J Southwest Univ Natl(Nat Sci),2016,42(1):52
[4]吴斌,吴立军.千里光属植物的化学成分研究进展[J].中国中药杂志,2003,28(2):6WU B,WU LJ.Research progress in chemical constituents of Senecio genus[J].Chin J Tradit Chin Med,2003,28(2):6
[5]韩贺东,胡海清,李琰,等.双花千里光中一个新的艾里莫芬烷型倍半萜[J].中国中药杂志,2013,38(19):3295HAN HD,HU HQ,LI Y,et al.A new erimophenane sesquiterpene in Senecio dianthus Franch.[J].China J Tradit Chin Med,2013,38(19):3295
[6]梅莹,黄帅,陈才,等.双花千里光中黄酮类成分的研究[J].华西药学杂志,2012,27(5):496MEI Y,HUANG S,CHEN C,et al.Study on flavonoids in Senecio dianthus Franch.[J].West China J Pharm Sci,2012,27(5):496
[7]中华人民共和国药典2015年版.一部[S].2015:33ChP 2015.VolⅠ[S].2015:33
[8]赵雪巍,刘培玉,刘丹,等.黄酮类化合物的构效关系研究进展[J].中草药,2015,46(21):3264ZHAO XW,LIU PY,LIU D,et al.Research progress in structureactivity relationship of flavonoids[J].Chin Tradit Herb Drugs,2015,46(21):3264
[9]李艳辉,格桑索朗,阿萍,等.双花千里光花精油化学成分的GC/MS分析[J].分析试验室,2006,25(7):42LI YH,Ge-Sang-Suo-Lang,A P,et al.GC/MS analysis of chemical constituents of Senecio dianthus Franch.essential oil[J].Chin JAnal Lab,2006,25(7):42
[10]蒋娅兰,毋福海,黄芳,等.高效液相色谱-串联质谱法同时测定山楂中6个黄酮类功效成分[J].药物分析杂志,2018,38(7):1139JIANG YL,WU FH,HUANG F,et al.Simultaneous determination of six flavonoids in hawthorn by high performance liquid chromatography-tandem mass spectrometry[J].Chin J Pharm Anal,2018,38(7):1139
[11]ARCINIEGAS A,GONZALEZ K,PEREZ-CASTORENA AL,et al.Seco-eremophiladiolides and eremophilane glucosides from Pittocaulon velatum[J].J Nat Prod,2011,74(7):1584
[12]黄帅.四种药用植物的化学成分及生物活性研究[D].成都:西南交通大学,2013HUANG S.Chemical Constituents and Biological Activities of Four Medicinal Plants[D].Chengdu:Southwest Jiaotong University,2013
[13]韩贺东,胡海清,林燕,等.双花千里光总黄酮的提取工艺优化及其抗氧化活性[J].食品与发酵工业,2013,39(7):232HAN HD,HU HQ,LIN Y,et al.Optimization of extraction process and antioxidant activity of total flavonoids from Senecio dianthus Franch.[J].Food Ferment Ind,2013,39(7):232