鹿产品鉴别的研究进展
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摘要
鹿产品是名贵中药材,亦可作为保健品,价格昂贵。目前市场上出现了许多杂交鹿的产品,还有其他以次充好、以假乱真的现象,不仅严重扰乱了市场秩序,侵犯了消费者的权益,同时也阻碍了中国鹿业的健康发展,因此鹿产品的鉴别变得非常重要。从历年来有关鹿产品鉴别的报道来看,现有的鉴别方法大致可分为性状鉴别、显微鉴别、理化鉴别和分子鉴别4种。本文对相关的报道作了汇总,为后续鹿产品鉴别的研究提供参考。
Deer products are precious Chinese medicinal materials. They are expensive and can be used as health care products. At present,many hybrid deer products appeared on the market and the phenomenon of shoddy is becoming worse and worse. Not only the market order is disrupted seriously,the rights and interests of consumers are violated,the healthy development of Chinese deer industry is hindered at the same time. So the identification of deer products is getting more and more vital. According to the reports on the identification of deer products over the years,the existing identification methods can be roughly divided into four types: morphological identification,microscopic identification,physical and chemical identification,and molecular identification. Reports related to deer product identification were summaried in this paper to provide a reference for the further study on identification of deer products.
Deer products are precious Chinese medicinal materials. They are expensive and can be used as health care products. At present,many hybrid deer products appeared on the market and the phenomenon of shoddy is becoming worse and worse. Not only the market order is disrupted seriously,the rights and interests of consumers are violated,the healthy development of Chinese deer industry is hindered at the same time. So the identification of deer products is getting more and more vital. According to the reports on the identification of deer products over the years,the existing identification methods can be roughly divided into four types: morphological identification,microscopic identification,physical and chemical identification,and molecular identification. Reports related to deer product identification were summaried in this paper to provide a reference for the further study on identification of deer products.
引文
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[2]高晓晨,刘冬,崔丽娜,等.市售鹿茸饮片及鹿茸粉的DNA条形码鉴定[J].吉林中医药,2016,36(7):706-708.
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[23]刘丽,康廷国.7种鹿茸茸毛的显微鉴别及系统聚类分析[J].中药材,2009,32(3):345-347.
[24]叶基荣,罗珍珠.从鞭毛的显微结构来鉴别鹿鞭的真伪[J].海峡药学,1992(2):12.
[25]郑广东,林敏,陈深.鹿鞭与其伪品假鹿鞭的鉴别[J].福建中医药,1998(3):37.
[26]李峰,刘春丽,李贺君,等.鹿鞭药材商品的显微鉴别研究[J].辽宁中医杂志,2010,37(8):1558-1559.
[27]胡翠英,刘传明,孟庆霞,等.光谱成像法鉴别鹿茸真伪的研究[J].中国医院药学杂志,2014,34(20):1751-1753,1784.
[28]吴信子,王思宏,吴丽花,等.不同鹿茸片红外数据的聚类分析[J].安徽农业科学,2010,38(35):20123-20124.
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[32]郭月秋,陈代贤,刘辉,等.鹿心及其伪品的免疫凝集试验鉴别探讨[J].中医药学报,2000(1):62-63.
[33]杨舒心,朱明娴,孙长江,等.鹿血双抗体夹心间接ELISA检测方法的建立[J].吉林农业大学学报,2010,32(4):440-442,452.
[34]朱云飞,初正云,李洪江.SDS-PAGE凝胶电泳法对不同种鹿茸蛋白质差异化研究[J].辽宁中医药大学学报,2017,19(6):48-51.
[35]金跃明,李峰.鹿鞭药材及伪品的SDS-聚丙烯酰胺凝胶电泳鉴别研究[J].辽宁中医学院学报,2005(6):623-624.
[36]郭月秋,陈代贤,刘辉,等.聚丙烯酰胺凝胶电泳鉴别鹿血与其它动物血[J].时珍国医国药,2000(3):232.
[37]郭月秋,陈代贤,任玮,等.鹿鞭与牛鞭的醋酸纤维素膜电泳鉴别[J].中药材,1998(12):611-612.
[38]郭月秋,陈代贤,李亚红,等.鹿胎及其伪充品的免疫醋酸纤维素膜电泳鉴别[J].中药材,1999(3):121-122.
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[42]张丽华,李明成,王冰梅,等.貂心线粒体mt DNA鉴定及特征分析[J].中国药学杂志,2008(22):1694-1696.
[43]唐双焱,傅文,陈永久,等.中药材鹿鞭的分子鉴定研究[J].中国中药杂志,2002(8):16-18.
[44]刘洋,张丽华.市售鹿鞭的DNA指纹鉴定研究[J].中国医药科学,2014,4(2):50-51,60.
[45]孙景昱,张丽华,傅桂莲,等.聚合酶链式反应偶联单链构象多态性分析(PCR-SSCP)技术用于鹿鞭线粒体DNA指纹的研究[J].中国药学杂志,2014,49(9):721-725.
[46]刘向华,王义权,周开亚,等.鹿类中药材的位点特异性PCR鉴定研究[J].药学学报,2001(8):631-635.
[47]王自强,吕晶,邵泓,等.利用限制性片段长度多态性技术鉴别梅花鹿骨种属来源的研究[J].中国药师,2017,20(5):813-816.
[48]崔丽娜,杜鹤,刘新成,等.基于COI条形码序列的鹿茸及其混伪品的DNA分子鉴定[J].吉林中医药,2012,32(4):384-387.
[49]陈康,蒋超,袁媛,等.基于熔解曲线分析技术的鹿茸药材分子鉴别[J].中国中药杂志,2015,40(4):619-623.
[50]张敏,白秀娟,罗付恒.利用SRY基因对梅花鹿茸和赤鹿茸进行鉴别[J].上海畜牧兽医通讯,2006(5):37.
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[2]高晓晨,刘冬,崔丽娜,等.市售鹿茸饮片及鹿茸粉的DNA条形码鉴定[J].吉林中医药,2016,36(7):706-708.
[3]唐双焱,傅文,陈永久,等.中药材鹿鞭的分子鉴定研究[J].中国中药杂志,2002(8):16-18.
[4]黄婧文.冬虫夏草及鹿茸真伪鉴别[J].实用中医药杂志,2011,27(1):60-61.
[5]刘国应.鹿茸的真伪鉴别[N].家庭医生报,2003-02-24(14).
[6]何连锋.鹿茸片的真伪鉴别[J].中国药业,2005(8):70-71.
[7]庞运同.鹿茸及其伪品的鉴别[J].中国误诊学杂志,2010,10(12):3013-3014.
[8]赵慧英,张颜.鹿胎的真伪鉴别[J].中药材,1985(1):18-19.
[9]陈代贤,郭月秋,门启鸣,等.鹿胎及其混伪品的性状鉴别研究[J].中药材,1997(5):226-229.
[10]李峰,康廷国.中药鹿鞭的研究进展[J].中医药学刊,2006(2):263-265.
[11]卢春蓉.鹿鞭的真伪鉴别[J].中国误诊学杂志,2002(3):460-461.
[12]李凤琴.几种伪制鹿鞭的比较鉴别[J].时珍国医国药,2003(10):615.
[13]李慧珍,李水福.鹿鞭的真伪优劣鉴定[J].中国药业,2008(10):66-67.
[14]肖家美,王峰,李红.鹿鞭的药理作用及其感官真伪鉴别[J].特产研究,2010,32(4):59-61,74.
[15]丛小松,于雯.鹿鞭正品与非正品的鉴别[J].中国中医药现代远程教育,2010,8(4):97.
[16]庞耀镜,黄汉游,李满联.鹿筋及其伪充品的性状鉴别[J].中药材,1993(11):13-14.
[17]吴淑玘.鹿筋及其伪品的鉴别[J].时珍国药研究,1995(3):21-22.
[18]贾宜军,周路山.鹿筋及其混伪品的性状鉴别[J].中药材,1994(7):17-18.
[19]刘邦强,余淑芳,王业发,等.鹿筋及其伪品的鉴别[J].时珍国医国药,2004(12):844.
[20]宏惠田,李桂兰.鹿茸片及其伪品的鉴定[J].天津药学,1994(4):32-34.
[21]陈代贤,郭月秋,任玮.商品等级鹿茸的显微组织鉴别[J].中药材,2000(9):526-528.
[22]陈代贤,郭月秋,任玮.11种鹿茸显微组织特征比较鉴别[J].中药材,2000(12):743-746.
[23]刘丽,康廷国.7种鹿茸茸毛的显微鉴别及系统聚类分析[J].中药材,2009,32(3):345-347.
[24]叶基荣,罗珍珠.从鞭毛的显微结构来鉴别鹿鞭的真伪[J].海峡药学,1992(2):12.
[25]郑广东,林敏,陈深.鹿鞭与其伪品假鹿鞭的鉴别[J].福建中医药,1998(3):37.
[26]李峰,刘春丽,李贺君,等.鹿鞭药材商品的显微鉴别研究[J].辽宁中医杂志,2010,37(8):1558-1559.
[27]胡翠英,刘传明,孟庆霞,等.光谱成像法鉴别鹿茸真伪的研究[J].中国医院药学杂志,2014,34(20):1751-1753,1784.
[28]吴信子,王思宏,吴丽花,等.不同鹿茸片红外数据的聚类分析[J].安徽农业科学,2010,38(35):20123-20124.
[29]吴新宇,王帅,尹冬冬.基于近红外光谱技术对鹿茸鉴别方法的研究[J].林业科技,2014,39(2):17-19.
[30]冯振波,乔宛虹.应用免疫学方法进行鹿鞭鉴别的研究[J].黑龙江中医药,1999(1):63.
[31]郭月秋,陈代贤,刘辉,等.鹿鞭及伪品的免疫凝集试验鉴别[J].中国药事,2000(2):31-33.
[32]郭月秋,陈代贤,刘辉,等.鹿心及其伪品的免疫凝集试验鉴别探讨[J].中医药学报,2000(1):62-63.
[33]杨舒心,朱明娴,孙长江,等.鹿血双抗体夹心间接ELISA检测方法的建立[J].吉林农业大学学报,2010,32(4):440-442,452.
[34]朱云飞,初正云,李洪江.SDS-PAGE凝胶电泳法对不同种鹿茸蛋白质差异化研究[J].辽宁中医药大学学报,2017,19(6):48-51.
[35]金跃明,李峰.鹿鞭药材及伪品的SDS-聚丙烯酰胺凝胶电泳鉴别研究[J].辽宁中医学院学报,2005(6):623-624.
[36]郭月秋,陈代贤,刘辉,等.聚丙烯酰胺凝胶电泳鉴别鹿血与其它动物血[J].时珍国医国药,2000(3):232.
[37]郭月秋,陈代贤,任玮,等.鹿鞭与牛鞭的醋酸纤维素膜电泳鉴别[J].中药材,1998(12):611-612.
[38]郭月秋,陈代贤,李亚红,等.鹿胎及其伪充品的免疫醋酸纤维素膜电泳鉴别[J].中药材,1999(3):121-122.
[39] Niedzialkowska M,Jdrzejewska B,Honnen A C,et al.Molecular biogeography of red deer Cervus elaphus from eastern Europe:insights from mitochondrial DNA sequences[J]. Acta Theriologica,2011,56(1):1-12.
[40] Meyer A,Wilson A C. Origin of tetrapods inferred from their mitochondrial DNA affiliation to lungfish[J].Journal of Molecular Evolution,1990,31(5):359-364.
[41] Kocher T D,Thomas W K,Meyer A,et al.Dynamics of mitochondrial DNA evolution in animals:amplification and sequencing with conserved primers.[J]. Proceedings of the National Academy of Sciences of the United States of America,1989,86(16):6196-6200.
[42]张丽华,李明成,王冰梅,等.貂心线粒体mt DNA鉴定及特征分析[J].中国药学杂志,2008(22):1694-1696.
[43]唐双焱,傅文,陈永久,等.中药材鹿鞭的分子鉴定研究[J].中国中药杂志,2002(8):16-18.
[44]刘洋,张丽华.市售鹿鞭的DNA指纹鉴定研究[J].中国医药科学,2014,4(2):50-51,60.
[45]孙景昱,张丽华,傅桂莲,等.聚合酶链式反应偶联单链构象多态性分析(PCR-SSCP)技术用于鹿鞭线粒体DNA指纹的研究[J].中国药学杂志,2014,49(9):721-725.
[46]刘向华,王义权,周开亚,等.鹿类中药材的位点特异性PCR鉴定研究[J].药学学报,2001(8):631-635.
[47]王自强,吕晶,邵泓,等.利用限制性片段长度多态性技术鉴别梅花鹿骨种属来源的研究[J].中国药师,2017,20(5):813-816.
[48]崔丽娜,杜鹤,刘新成,等.基于COI条形码序列的鹿茸及其混伪品的DNA分子鉴定[J].吉林中医药,2012,32(4):384-387.
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