高温胁迫对金露梅叶片结构和生理代谢的影响
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摘要
以3年生金露梅叶片为试材,在42℃下持续3d高温胁迫,研究了高温对其叶片叶绿体超微结构、叶绿素荧光参数、细胞膜透性、渗透物质含量、抗氧化酶活性的影响。结果表明:随着高温胁迫时间的延长,金露梅的叶绿体发生严重的解体现象;叶片最大光化学效率(Fv/Fm)呈下降趋势,非光化学猝灭系数(qN)和光化学猝灭系数(qP)先上升后下降。细胞膜透性及丙二醛(MDA)、游离脯氨酸、可溶性糖含量呈上升趋势,且显著高于对照(未进行高温胁迫);超氧化物歧化酶(SOD)活性无明显变化,过氧化氢酶(CAT)活性呈先上升后下降趋势。表明在持续高温胁迫下,金露梅叶片受到损伤,但植物体内会通过渗透物质及抗氧化酶来缓解高温伤害。
3-year-old Potentilla fruticosa L.was taken as material and treated at 42 ℃for 3 days.The effects of high temperature on chloroplast ultra-structures of leaf,chlorophyll fluorescence parameters,the membrane permetability,osmotic regulation substances and antioxidant enzyme activities of Potentilla fruticosa L.were studied.The results showed that with the extension of high temperature stress time,the chloroplast showed severe degradation and the maximal photochemical efficiency Fv/Fm reduced significantly,while the non-photochemical quenching(qN)and the photochemical quenching(qP)first rose then declined.The membrane permetability,the contents of MDA,free proline and soluble sugar increased gradually,and all the indicators were significantly higher than those of control(no high temperature stress).While the activity of SOD showed no obvious change,and the activity of CAT first rose and then declined.The leaves of P.fruticosa suffered seriously damaged with the high temperature stress,but the plant could relieve heat damage through osmotic regulation substances and antioxidant enzyme.
3-year-old Potentilla fruticosa L.was taken as material and treated at 42 ℃for 3 days.The effects of high temperature on chloroplast ultra-structures of leaf,chlorophyll fluorescence parameters,the membrane permetability,osmotic regulation substances and antioxidant enzyme activities of Potentilla fruticosa L.were studied.The results showed that with the extension of high temperature stress time,the chloroplast showed severe degradation and the maximal photochemical efficiency Fv/Fm reduced significantly,while the non-photochemical quenching(qN)and the photochemical quenching(qP)first rose then declined.The membrane permetability,the contents of MDA,free proline and soluble sugar increased gradually,and all the indicators were significantly higher than those of control(no high temperature stress).While the activity of SOD showed no obvious change,and the activity of CAT first rose and then declined.The leaves of P.fruticosa suffered seriously damaged with the high temperature stress,but the plant could relieve heat damage through osmotic regulation substances and antioxidant enzyme.
引文
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[10]金春燕,郭世荣,施标,等.高温对不同番茄品种幼苗叶片抗氧化系统和渗透调节物质的影响[J].上海农业学报,2011,27(3):26-30.
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[12]查倩,奚晓军,蒋爱丽,等.高温胁迫对葡萄幼树叶绿素荧光特性和抗氧化酶活性的影响[J].植物生理学报,2016(4):525-532.
[13]段九菊,王云山,康黎芳,等.高温胁迫对观赏凤梨叶片抗氧化系统和渗透调节物质积累的影响[J].中国农学通报,2010,26(8):164-169.
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[15]张顺堂,张桂莲,陈立云,等.高温胁迫对水稻剑叶净光合速率和叶绿素荧光参数的影响[J].中国水稻科学,2011,25(3):335-338.
[16]郑健,郑勇奇,苑林,等.金露梅扦插繁殖技术研究[J].林业科学研究,2007,20(5):736-738.
[17]郑健,张彦广.野生花卉金露梅种子萌发特性研究[J].河北科技师范学院学报,2004,18(2):62-64.
[18]李春雁,王晓群,程立华,等.金露梅叶中总黄酮含量分析[J].中医药信息,2001,18(4):55-55.
[19]季志平,张绒仙,康永祥,等.太白山金露梅叶总黄酮含量分析[J].陕西林业科技,2011(2):1-2.
[20]杨时海,李英年,蒲继延,等.三种高寒草甸植被类型植物群落结构及其土壤环境因子研究[J].草地学报,2006,14(1):77-83.
[21]赵君,张仁懿,李新娥,等.甘南亚高寒草甸金露梅叶片氮磷化学计量学动态[J].兰州大学学报(自然科学版),2011,47(2):88-92.
[22]刘行,张彦广,夏群.Na2SO4胁迫对金露梅生理特性的影响[J].林业工程学报,2014,28(6):30-34.
[23]刘行,张彦广.盐胁迫对金露梅叶片酶活性和叶绿素含量的影响[J].西部林业科学,2016,45(4):95-100.
[24]蔡庆生.植物生理学实验[M].北京:中国农业大学出版社,2013:175-176.
[25]李晓锋,侯瑞贤,朱玉英,等.高温胁迫对大白菜叶绿素荧光特性的影响[J].农业工程学报,2009(S1):49-54.
[26]黄磊,孙耀清,郝立华,等.高温对北高丛越橘叶片结构和生理代谢的影响[J].园艺学报,2016,43(6):1044-1056.
[27]XU S,LI J,ZHANG X,et al.Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation,antioxidant metabolites,and ultrastructure of chloroplasts in two coolseason turfgrass species under heat stress[J].Environmental&Experimental Botany,2006,56(3):274-285.
[28]商侃侃,张德顺,王铖.高温胁迫下植物抗性生理研究进展[J].园林科技,2008(1):1-5,42.
[29]QIU N,LU C.Enhanced tolerance of photosynthesis against high temperature damage in saltr heat stress[J].Plant Cell&Environment,2003,26(7):1137-1145.
[30]李静,徐志防,叶万辉,等.不同胁迫处理对刺栲叶片叶绿素a荧光的影响[J].植物科学学报,2006,24(5):429-434.
[31]LUO Y,LI W M,WANG W.Trehalose:Protector of antioxidant enzymes or reactive oxygen species scavenger under heat stress?[J].Environmental&Experimental Botany,2008,63(1):378-384.
[32]GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology&Biochemistry,2010,48(12):909.
[33]KUMAR N,SHANKHDHAR S C,SHANKHDHAR D.Impact of elevated temperature on antioxidant activity and membrane stability in different genotypes of rice(Oryza sativa L.)[J].Indian Journal of Plant Physiology,2016,21(1):37-43.
[34]张显强,罗在柒,唐金刚,等.高温和干旱胁迫对鳞叶藓游离脯氨酸和可溶性糖含量的影响[J].广西植物,2004,24(6):570-573.
[35]刘磊,陈立波,李志勇,等.晚秋温度对苜蓿地上部游离脯氨酸、可溶性糖和POD活性的影响[J].草业科学,2009,26(10):89-93.
[36]PAUL S,GOGOI N,SARMA B,et al.Biochemical changes in potato under elevated temperature[J].Indian Journal of Plant Physiology,2014,19(1):36-42.
[2]刘悦萍,黄卫东,张俊环.高温锻炼和水杨酸预处理对热激下葡萄叶肉细胞超微结构的影响[J].园艺学报,2006,33(3):491-495.
[3]王涛,田雪瑶,谢寅峰,等.植物耐热性研究进展[J].云南农业大学学报,2013,28(5):719-726.
[4] KRAUSE G H,SANTARIUS K A.Relative thermostability of the chloroplast envelope[J].Planta,1975,127(3):285-299.
[5]王光耀,刘俊梅,张仪,等.热锻炼和热胁迫过程中菜豆叶肉细胞超微结构的变化[J].农业生物技术学报,1999,7(2):151-156.
[6]陈燕,郑小林,曾富华,等.高温干旱下两种冷季型草坪草叶片细胞超微结构的变化[J].西北植物学报,2003,23(2):304-308.
[7]屠小菊,汪启明,饶力群.高温胁迫对植物生理生化的影响[J].湖南农业科学,2013(13):28-30.
[8]张哲,闵红梅,夏关均,等.高温胁迫对植物生理影响研究进展[J].安徽农业科学,2010,38(16):8338-8339,8342.
[9]王志勇,廖丽,林文雄.高温对高羊茅叶片中渗透调节物质的影响[J].江西农业大学学报,2007,29(2):176-181.
[10]金春燕,郭世荣,施标,等.高温对不同番茄品种幼苗叶片抗氧化系统和渗透调节物质的影响[J].上海农业学报,2011,27(3):26-30.
[11]赵森,于江辉,肖国樱.高温胁迫对爪哇稻剑叶光合特性和渗透调节物质的影响[J].生态环境学报,2013(1):110-115.
[12]查倩,奚晓军,蒋爱丽,等.高温胁迫对葡萄幼树叶绿素荧光特性和抗氧化酶活性的影响[J].植物生理学报,2016(4):525-532.
[13]段九菊,王云山,康黎芳,等.高温胁迫对观赏凤梨叶片抗氧化系统和渗透调节物质积累的影响[J].中国农学通报,2010,26(8):164-169.
[14]杜尧东,李键陵,王华,等.高温胁迫对水稻剑叶光合和叶绿素荧光特征的影响[J].生态学杂志,2012,31(10):2541-2548.
[15]张顺堂,张桂莲,陈立云,等.高温胁迫对水稻剑叶净光合速率和叶绿素荧光参数的影响[J].中国水稻科学,2011,25(3):335-338.
[16]郑健,郑勇奇,苑林,等.金露梅扦插繁殖技术研究[J].林业科学研究,2007,20(5):736-738.
[17]郑健,张彦广.野生花卉金露梅种子萌发特性研究[J].河北科技师范学院学报,2004,18(2):62-64.
[18]李春雁,王晓群,程立华,等.金露梅叶中总黄酮含量分析[J].中医药信息,2001,18(4):55-55.
[19]季志平,张绒仙,康永祥,等.太白山金露梅叶总黄酮含量分析[J].陕西林业科技,2011(2):1-2.
[20]杨时海,李英年,蒲继延,等.三种高寒草甸植被类型植物群落结构及其土壤环境因子研究[J].草地学报,2006,14(1):77-83.
[21]赵君,张仁懿,李新娥,等.甘南亚高寒草甸金露梅叶片氮磷化学计量学动态[J].兰州大学学报(自然科学版),2011,47(2):88-92.
[22]刘行,张彦广,夏群.Na2SO4胁迫对金露梅生理特性的影响[J].林业工程学报,2014,28(6):30-34.
[23]刘行,张彦广.盐胁迫对金露梅叶片酶活性和叶绿素含量的影响[J].西部林业科学,2016,45(4):95-100.
[24]蔡庆生.植物生理学实验[M].北京:中国农业大学出版社,2013:175-176.
[25]李晓锋,侯瑞贤,朱玉英,等.高温胁迫对大白菜叶绿素荧光特性的影响[J].农业工程学报,2009(S1):49-54.
[26]黄磊,孙耀清,郝立华,等.高温对北高丛越橘叶片结构和生理代谢的影响[J].园艺学报,2016,43(6):1044-1056.
[27]XU S,LI J,ZHANG X,et al.Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation,antioxidant metabolites,and ultrastructure of chloroplasts in two coolseason turfgrass species under heat stress[J].Environmental&Experimental Botany,2006,56(3):274-285.
[28]商侃侃,张德顺,王铖.高温胁迫下植物抗性生理研究进展[J].园林科技,2008(1):1-5,42.
[29]QIU N,LU C.Enhanced tolerance of photosynthesis against high temperature damage in saltr heat stress[J].Plant Cell&Environment,2003,26(7):1137-1145.
[30]李静,徐志防,叶万辉,等.不同胁迫处理对刺栲叶片叶绿素a荧光的影响[J].植物科学学报,2006,24(5):429-434.
[31]LUO Y,LI W M,WANG W.Trehalose:Protector of antioxidant enzymes or reactive oxygen species scavenger under heat stress?[J].Environmental&Experimental Botany,2008,63(1):378-384.
[32]GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology&Biochemistry,2010,48(12):909.
[33]KUMAR N,SHANKHDHAR S C,SHANKHDHAR D.Impact of elevated temperature on antioxidant activity and membrane stability in different genotypes of rice(Oryza sativa L.)[J].Indian Journal of Plant Physiology,2016,21(1):37-43.
[34]张显强,罗在柒,唐金刚,等.高温和干旱胁迫对鳞叶藓游离脯氨酸和可溶性糖含量的影响[J].广西植物,2004,24(6):570-573.
[35]刘磊,陈立波,李志勇,等.晚秋温度对苜蓿地上部游离脯氨酸、可溶性糖和POD活性的影响[J].草业科学,2009,26(10):89-93.
[36]PAUL S,GOGOI N,SARMA B,et al.Biochemical changes in potato under elevated temperature[J].Indian Journal of Plant Physiology,2014,19(1):36-42.