新疆两色金鸡菊中原花青素含量测定体系考察
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摘要
为准确测定新疆两色金鸡中原花青素含量,选择合适的对照品和测定体系。通过比较香草醛-盐酸、正丁醇-盐酸、铁盐(Fe~(3+))催化法、pH示差法等多种测定体系下,两色金鸡菊提取物和对照品儿茶素或原花青素的连续波长扫描图谱,考察光谱吸收曲线的一致性和λ_(max)峰位值,进而选择适合测定两色金鸡菊中原花青素含量的显色反应体系。结果表明,两色金鸡菊提取物与原花青素对照品在正丁醇-盐酸、Fe~(3+)催化法和pH示差法产生的光谱吸收曲线相似,λ_(max)峰位值更接近。因此,可以用这3种体系快速测定两色金鸡菊中原花青素的含量。结果显示,在特定的测定体系中,选择合适的对照品能准确测定新疆两色金鸡菊中原花青素的含量。
To select the appropriate standards and chromogenic reaction system for determination the content of proanthocyanidins in Coreopsis tinctoria flowering tops(CTFT)from Xinjiang,the spectrum of CTFT extract,catechin and proanthocyanidins standards were compared in vanillin-hydrochloric acid,n-butanol-hydrochloric acid,Fe~(3+) catalytic chromogenic method and pH differential spectrophotometry reaction system. The spectral absorption curve and the wavelength λ_(max)peak values were investigated for selecting a suitable reaction system to determine the content of proanthocyanidins of CTFT. The spectrum of CTFT extract and proanthocyanidins standard were similar in n-butanol-hydrochloric acid,Fe~(3+) chromogenic method and pH differential spectrophotometry reaction system,and the λ_(max)values were also closer to standard. Therefore,the content of proanthocyanidins in CTFT could be assayed by these three chromogenic reaction system. The content of proanthocyanidins in CTFT could be accurately determined in a particular reaction system and using an appropriate standard.
To select the appropriate standards and chromogenic reaction system for determination the content of proanthocyanidins in Coreopsis tinctoria flowering tops(CTFT)from Xinjiang,the spectrum of CTFT extract,catechin and proanthocyanidins standards were compared in vanillin-hydrochloric acid,n-butanol-hydrochloric acid,Fe~(3+) catalytic chromogenic method and pH differential spectrophotometry reaction system. The spectral absorption curve and the wavelength λ_(max)peak values were investigated for selecting a suitable reaction system to determine the content of proanthocyanidins of CTFT. The spectrum of CTFT extract and proanthocyanidins standard were similar in n-butanol-hydrochloric acid,Fe~(3+) chromogenic method and pH differential spectrophotometry reaction system,and the λ_(max)values were also closer to standard. Therefore,the content of proanthocyanidins in CTFT could be assayed by these three chromogenic reaction system. The content of proanthocyanidins in CTFT could be accurately determined in a particular reaction system and using an appropriate standard.
引文
[1]新疆植物志编辑委员会.新疆植物志(第5卷)[M].乌鲁木齐:新疆科技卫生出版社,1999:93
[2]姚新成,田丽萍,秦冬梅,等.两色金鸡菊化学成分及生物活性研究进展[J].西北药学杂志,2014,29(6):655-658
[3]姜保平,许利嘉,贾晓光,等.两色金鸡菊的化学成分和药理作用研究进展[J].现代药物与临床,2014,29(5):567-573
[3]姚开,何强,吕远平,等.葡萄籽提取物中原花青素含量的测定[J].食品与发酵工业,2002,28(3):17-19
[4]晏小欣,木尼热·阿不都克里木,敬思群.金鸡菊黄色素的提取及抗氧化性的研究[J].中国酿造,2012,31(5):144-148
[5]张璐,潘佩佩,陈赛贞.RP-HPLC法同时测定原花青素中儿茶素、表儿茶素、没食子酸、原花青素B2的含量[J].中国药科大学学报,2016,47(1):54-57
[6]赵平,刘俊英,张月萍.香草醛法测定原花青素物质的量浓度[J].中国食品添加剂,2011(3):219-224
[7]姚开,何强,吕远平,等.葡萄籽提取物中原花青素含量不同测定方法比较[J].化学研究与应用,2002,14(2):230-232
[8]杜利君,姚亚婷,王静慧,等.改进铁盐催化比色法测定保健食品中原花青素[J].检验检疫学刊2013,23(5):50-52
[9]王少波,杜永峰,姚秉华.pH示差法测定黑豆皮中的花青素[J].化学分析计量,2008,17(1):46-47
[10]杨兆艳.pH示差法测定桑椹红色素中花青素含量的研究[J].食品科技,2007,32(4):201-203
[2]姚新成,田丽萍,秦冬梅,等.两色金鸡菊化学成分及生物活性研究进展[J].西北药学杂志,2014,29(6):655-658
[3]姜保平,许利嘉,贾晓光,等.两色金鸡菊的化学成分和药理作用研究进展[J].现代药物与临床,2014,29(5):567-573
[3]姚开,何强,吕远平,等.葡萄籽提取物中原花青素含量的测定[J].食品与发酵工业,2002,28(3):17-19
[4]晏小欣,木尼热·阿不都克里木,敬思群.金鸡菊黄色素的提取及抗氧化性的研究[J].中国酿造,2012,31(5):144-148
[5]张璐,潘佩佩,陈赛贞.RP-HPLC法同时测定原花青素中儿茶素、表儿茶素、没食子酸、原花青素B2的含量[J].中国药科大学学报,2016,47(1):54-57
[6]赵平,刘俊英,张月萍.香草醛法测定原花青素物质的量浓度[J].中国食品添加剂,2011(3):219-224
[7]姚开,何强,吕远平,等.葡萄籽提取物中原花青素含量不同测定方法比较[J].化学研究与应用,2002,14(2):230-232
[8]杜利君,姚亚婷,王静慧,等.改进铁盐催化比色法测定保健食品中原花青素[J].检验检疫学刊2013,23(5):50-52
[9]王少波,杜永峰,姚秉华.pH示差法测定黑豆皮中的花青素[J].化学分析计量,2008,17(1):46-47
[10]杨兆艳.pH示差法测定桑椹红色素中花青素含量的研究[J].食品科技,2007,32(4):201-203